Concanavalin A-induced T-cell-mediated hepatic injury in mice: the role of tumor necrosis factor - PubMed (original) (raw)
Concanavalin A-induced T-cell-mediated hepatic injury in mice: the role of tumor necrosis factor
F Gantner et al. Hepatology. 1995 Jan.
Abstract
Concanavalin A activates T lymphocytes in vitro and causes T-cell-dependent hepatic injury in mice. T lymphocytes were previously identified as effector cells of concanavalin A-induced liver injury. Here we report that hepatic injury is characterized by apoptotic cell death. On concanavalin A challenge, the cytokines tumor necrosis factor-alpha (TNF alpha), interleukin-2, granulocyte macrophage-colony stimulating factor, and interferon-gamma were detectable in the circulation of the mice. Pretreatment of mice with anti-mouse TNF-alpha antiserum protected them from concanavalin A-induced liver injury. Nude mice failed to release TNF-alpha or interleukin-2 after concanavalin A challenge and were protected from liver injury. Lymph node cell transfer from responder mice to resistant nude mice resulted in susceptibility of the latter towards concanavalin A, i.e., to induction of cytokine release and hepatotoxicity. These experiments suggest that immunocompetent T cells play a pivotal role in concanavalin A-stimulated TNF-alpha release in vivo. After intravenous administration of fluorescein isothiocyanate-labeled concanavalin A to mice, the most fluorescence was found within the liver. In vitro, concanavalin A stimulation of separate cultures of mouse lymph node cells or nonparenchymal liver cells induced the release of minute amounts of TNF, whereas stimulation of cocultures of these cells resulted in production of substantial amounts of TNF-alpha. These findings may explain the hepatotropic effect of concanavalin A. In conclusion, T-cell-dependent concanavalin A-induced apoptotic liver injury in mice is related to immunological and cytokine-mediated disorders and possibly to autoreactive hepatic processes.
Similar articles
- Interferon gamma plays a critical role in T cell-dependent liver injury in mice initiated by concanavalin A.
Küsters S, Gantner F, Künstle G, Tiegs G. Küsters S, et al. Gastroenterology. 1996 Aug;111(2):462-71. doi: 10.1053/gast.1996.v111.pm8690213. Gastroenterology. 1996. PMID: 8690213 - Effects of intravenous immunoglobulins on T-cell mediated, concanavalin A-induced hepatitis in mice.
Shirin H, Bruck R, Aeed H, Hershkoviz R, Lider O, Kenet G, Avni Y, Halpern Z. Shirin H, et al. Liver. 1997 Dec;17(6):275-80. doi: 10.1111/j.1600-0676.1997.tb01032.x. Liver. 1997. PMID: 9455732 - T cell stimulus-induced crosstalk between lymphocytes and liver macrophages results in augmented cytokine release.
Gantner F, Leist M, Küsters S, Vogt K, Volk HD, Tiegs G. Gantner F, et al. Exp Cell Res. 1996 Nov 25;229(1):137-46. doi: 10.1006/excr.1996.0351. Exp Cell Res. 1996. PMID: 8940257 - Experimental hepatitis and role of cytokines.
Tiegs G. Tiegs G. Acta Gastroenterol Belg. 1997 Apr-Jun;60(2):176-9. Acta Gastroenterol Belg. 1997. PMID: 9260332 Review. - Molecular pathogenesis of T lymphocyte-induced liver injury in alcoholic hepatitis.
Batey RG, Wang J. Batey RG, et al. Front Biosci. 2002 Jul 1;7:d1662-75. doi: 10.2741/A870. Epub 2002 Jul 1. Front Biosci. 2002. PMID: 12086911 Review.
Cited by
- The ST2+ Treg/amphiregulin axis protects from immune-mediated hepatitis.
Wachtendorf S, Jonin F, Ochel A, Heinrich F, Westendorf AM, Tiegs G, Neumann K. Wachtendorf S, et al. Front Immunol. 2024 Mar 20;15:1351405. doi: 10.3389/fimmu.2024.1351405. eCollection 2024. Front Immunol. 2024. PMID: 38571949 Free PMC article. - CD73 mediates the therapeutic effects of endometrial regenerative cells in concanavalin A-induced hepatitis by regulating CD4+ T cells.
Qin H, Sun C, Kong D, Zhu Y, Shao B, Ren S, Wang H, Zhang J, Xu Y, Wang H. Qin H, et al. Stem Cell Res Ther. 2023 Sep 29;14(1):277. doi: 10.1186/s13287-023-03505-2. Stem Cell Res Ther. 2023. PMID: 37775797 Free PMC article. - The multifaceted role of macrophages during acute liver injury.
Hassan GS, Flores Molina M, Shoukry NH. Hassan GS, et al. Front Immunol. 2023 Sep 6;14:1237042. doi: 10.3389/fimmu.2023.1237042. eCollection 2023. Front Immunol. 2023. PMID: 37736102 Free PMC article. Review. - Erythropoietin Suppresses the Hepatic Fibrosis Caused by Thioacetamide: Role of the PI3K/Akt and TLR4 Signaling Pathways.
Elbaset MA, Mohamed BMSA, Moustafa PE, Mansour DF, Afifi SM, Esatbeyoglu T, Abdelrahman SSM, Fayed HM. Elbaset MA, et al. Oxid Med Cell Longev. 2023 Aug 22;2023:5514248. doi: 10.1155/2023/5514248. eCollection 2023. Oxid Med Cell Longev. 2023. PMID: 37649466 Free PMC article. - Sterile inflammation in liver transplantation.
Kahan R, Cray PL, Abraham N, Gao Q, Hartwig MG, Pollara JJ, Barbas AS. Kahan R, et al. Front Med (Lausanne). 2023 Aug 10;10:1223224. doi: 10.3389/fmed.2023.1223224. eCollection 2023. Front Med (Lausanne). 2023. PMID: 37636574 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources