Replication of the genomic RNA of a positive-strand RNA animal virus from negative-sense transcripts - PubMed (original) (raw)

Replication of the genomic RNA of a positive-strand RNA animal virus from negative-sense transcripts

L A Ball. Proc Natl Acad Sci U S A. 1994.

Abstract

Studies of RNA replication among the positive-strand RNA animal viruses have been hindered by the apparent inability of their RNA-dependent RNA polymerases to initiate replication on the corresponding negative-sense RNAs. However, here I report that in the case of the nodavirus flock house virus (FHV), which has a bipartite positive-sense RNA genome, the viral RNA replicase can replicate a negative-sense transcript of the genome segment that encodes the viral capsid proteins. For this work, the FHV replication cycle was experimentally reconstructed in baby hamster kidney cells that were transfected with specialized transcription plasmids designed to direct the synthesis of RNAs which corresponded closely to the two genome segments of FHV. The RNA replicase encoded by the larger genome segment could utilize either the positive or the negative strand of the smaller segment as a template, and it catalyzed RNA replication to produce similar RNA products in the two situations. Surprisingly, studies of the nucleotide sequences that were required for replication showed that the 3' end of the negative-strand RNA contained only a minimal cis-acting signal. The success of these experiments will facilitate further studies of the cis- and trans-acting factors involved in the recognition and replication of negative-sense RNA in this system.

PubMed Disclaimer

Cited by

Natural and experimental infection of Caenorhabditis nematodes by novel viruses related to nodaviruses.
Félix MA, Ashe A, Piffaretti J, Wu G, Nuez I, Bélicard T, Jiang Y, Zhao G, Franz CJ, Goldstein LD, Sanroman M, Miska EA, Wang D. Félix MA, et al. PLoS Biol. 2011 Jan 25;9(1):e1000586. doi: 10.1371/journal.pbio.1000586. PLoS Biol. 2011. PMID: 21283608 Free PMC article.
Nodavirus-induced membrane rearrangement in replication complex assembly requires replicase protein a, RNA templates, and polymerase activity.
Kopek BG, Settles EW, Friesen PD, Ahlquist P. Kopek BG, et al. J Virol. 2010 Dec;84(24):12492-503. doi: 10.1128/JVI.01495-10. Epub 2010 Oct 13. J Virol. 2010. PMID: 20943974 Free PMC article.
CCA initiation boxes without unique promoter elements support in vitro transcription by three viral RNA-dependent RNA polymerases.
Yoshinari S, Nagy PD, Simon AE, Dreher TW. Yoshinari S, et al. RNA. 2000 May;6(5):698-707. doi: 10.1017/s1355838200992410. RNA. 2000. PMID: 10836791 Free PMC article.
In vivo DNA expression of functional brome mosaic virus RNA replicons in Saccharomyces cerevisiae.
Ishikawa M, Janda M, Krol MA, Ahlquist P. Ishikawa M, et al. J Virol. 1997 Oct;71(10):7781-90. doi: 10.1128/JVI.71.10.7781-7790.1997. J Virol. 1997. PMID: 9311863 Free PMC article.
Characterization of a nodavirus replicase revealed a de novo initiation mechanism of RNA synthesis and terminal nucleotidyltransferase activity.
Wang Z, Qiu Y, Liu Y, Qi N, Si J, Xia X, Wu D, Hu Y, Zhou X. Wang Z, et al. J Biol Chem. 2013 Oct 25;288(43):30785-801. doi: 10.1074/jbc.M113.492728. Epub 2013 Sep 9. J Biol Chem. 2013. PMID: 24019510 Free PMC article.

References

1. J Virol. 1993 Jun;67(6):3544-51 - PubMed
1. Cell. 1992 Jun 12;69(6):1011-20 - PubMed
1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
1. Curr Top Microbiol Immunol. 1983;105:1-98 - PubMed
1. J Gen Virol. 1994 May;75 ( Pt 5):1009-14 - PubMed

Replication of the genomic RNA of a positive-strand RNA animal virus from negative-sense transcripts - PubMed (original) (raw)