Human proteasome subunits from 2-dimensional gels identified by partial sequencing - PubMed (original) (raw)
. 1994 Dec 30;205(3):1785-9.
doi: 10.1006/bbrc.1994.2876.
Affiliations
- PMID: 7811265
- DOI: 10.1006/bbrc.1994.2876
Human proteasome subunits from 2-dimensional gels identified by partial sequencing
P Kristensen et al. Biochem Biophys Res Commun. 1994.
Erratum in
- Human proteasome subunits from 2-dimensional gels identified by partial sequencing.
Kristensen P, Johnsen AH, Uerkvitz W, Tanaka K, Hendil KB. Kristensen P, et al. Biochem Biophys Res Commun. 1995 Feb 27;207(3):1059. doi: 10.1006/bbrc.1995.1294. Biochem Biophys Res Commun. 1995. PMID: 7864893 No abstract available.
Abstract
Human proteasomes consist of 14 major subunits which can be resolved by two-dimensional polyacrylamide gel electrophoresis. Tryptic peptides from the subunits were sequenced. This allowed identification of all proteins in two-dimensional electrophoresis gels with proteasome subunits whose sequences are known from cDNA. The results also precisely define the specificity of a panel of subunit-specific monoclonal antibodies. A new proteasome subunit (Z) was discovered. In contrast, the putative subunit MECL-1 could not be detected in human placenta proteasomes.
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