Proteinase-resistant factors in human erythrocyte membranes mediate CD4-dependent fusion with cells expressing human immunodeficiency virus type 1 envelope glycoproteins - PubMed (original) (raw)

Proteinase-resistant factors in human erythrocyte membranes mediate CD4-dependent fusion with cells expressing human immunodeficiency virus type 1 envelope glycoproteins

T Dragic et al. J Virol. 1995 Feb.

Abstract

Murine CD4+ cells are resistant to human immunodeficiency virus type 1 (HIV-1) entry and to fusion with cells expressing HIV-1 envelope glycoproteins (Env). The role of human-specific factors in Env/CD4-mediated fusion is shown by the ability of transient cell hybrids formed between CD4+ murine cells and human HeLa cells to fuse with Env+ cells. Fusion events were observed when other human cells, including erythrocytes, were substituted for HeLa cells in the hybrids. Experiments with erythrocyte ghosts showed that the factors allowing Env/CD4-mediated fusion are located in the plasma membrane. These factors were fully active after extensive digestion of erythrocytes with proteinase K or pronase. Nonprotein components of human plasma membranes, possibly glycolipids, could therefore be required for Env/CD4-mediated fusion and virus entry.

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References

    1. J Virol. 1990 Jan;64(1):215-21 - PubMed
    1. Cell. 1986 Nov 7;47(3):333-48 - PubMed
    1. Virology. 1989 Feb;168(2):267-73 - PubMed
    1. FEBS Lett. 1989 May 8;248(1-2):48-52 - PubMed
    1. Science. 1989 Jun 2;244(4908):1075-8 - PubMed

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