Genotypic subtypes of HIV-1 in Cameroon - PubMed (original) (raw)

Comparative Study

Genotypic subtypes of HIV-1 in Cameroon

J N Nkengasong et al. AIDS. 1994 Oct.

Abstract

Objective: The only two HIV-1 strains (ANT70 and MVP5180) reported to date from Cameroon are members of the outlier clade (group O). In this study, we assessed the prevalence of group O viruses and other HIV-1 subtypes in Cameroon.

Design: A phylogenetic analysis of 18 HIV-1 strains isolated from seropositive individuals from Yaoundé and Douala, Cameroon.

Methods: A 900 base-pair fragment of the env gene coding for V3, V4, V5, and the beginning of gp41 of 17 out of 18 HIV-1 isolates from Cameroon was amplified, cloned and sequenced using polymerase chain reaction. A phylogenetic tree was constructed.

Results: The overall env nucleotide sequence divergence among the Cameroon isolates ranged from 6.1 to 27.5%. In a phylogenetic tree, six subtypes were identified when compared with 23 reference strains of different geographic origin. Of these 17 Cameroonian strains, 11 (61%) were of subtype A of which the interpatient distances at the sequence level varied from 6.1% to 18.3% (average, 11.9%). Three (17%) strains were of subtype F, and the other three strains (6% each) belonged to subtypes B, E and H, respectively. The remaining isolate was classified as belonging to group O, on the basis of the sequence of part of the pol gene. A very broad spectrum of different tetrameric amino-acid sequences was observed at the apex of the V3 loop. Eleven strains contained the tetrameric globally predominant GPGQ sequence at the tip of the V3 motif. Two strains had the GPGR sequence typical of the American and European HIV-1 strains. The remaining tetrameric sequences included GPGS, GSGQ, GRGQ, and GLGR.

Conclusion: These findings on a limited number of viruses suggest extensive env gene diversity of HIV-1 strains from Cameroon, and could have implications for vaccine development in Africa.

PubMed Disclaimer

Cited by

Near full genome characterization of HIV-1 unique recombinant forms in Cameroon reveals dominant CRF02_AG and F2 recombination patterns.
Banin AN, Tuen M, Bimela JS, Tongo M, Zappile P, Khodadadi-Jamayran A, Nanfack AJ, Okonko IO, Meli J, Wang X, Mbanya D, Ngogang J, Gorny MK, Heguy A, Fokunang C, Duerr R. Banin AN, et al. J Int AIDS Soc. 2019 Jul;22(7):e25362. doi: 10.1002/jia2.25362. J Int AIDS Soc. 2019. PMID: 31353798 Free PMC article.
Characterization of a highly replicative intergroup M/O human immunodeficiency virus type 1 recombinant isolated from a Cameroonian patient.
Peeters M, Liegeois F, Torimiro N, Bourgeois A, Mpoudi E, Vergne L, Saman E, Delaporte E, Saragosti S. Peeters M, et al. J Virol. 1999 Sep;73(9):7368-75. doi: 10.1128/JVI.73.9.7368-7375.1999. J Virol. 1999. PMID: 10438826 Free PMC article.
Comparison of predicted scaffold-compatible sequence variation in the triple-hairpin structure of human imunodeficiency virus type 1 gp41 with patient data.
Boutonnet N, Janssens W, Boutton C, Verschelde JL, Heyndrickx L, Beirnaert E, van der Groen G, Lasters I. Boutonnet N, et al. J Virol. 2002 Aug;76(15):7595-606. doi: 10.1128/jvi.76.15.7595-7606.2002. J Virol. 2002. PMID: 12097573 Free PMC article.
Analysis of pol gene heterogeneity, viral quasispecies, and drug resistance in individuals infected with group O strains of human immunodeficiency virus type 1.
Quiñones-Mateu ME, Albright JL, Mas A, Soriano V, Arts EJ. Quiñones-Mateu ME, et al. J Virol. 1998 Nov;72(11):9002-15. doi: 10.1128/JVI.72.11.9002-9015.1998. J Virol. 1998. PMID: 9765445 Free PMC article.
Mapping of epitopes exposed on intact human immunodeficiency virus type 1 (HIV-1) virions: a new strategy for studying the immunologic relatedness of HIV-1.
Nyambi PN, Gorny MK, Bastiani L, van der Groen G, Williams C, Zolla-Pazner S. Nyambi PN, et al. J Virol. 1998 Nov;72(11):9384-91. doi: 10.1128/JVI.72.11.9384-9391.1998. J Virol. 1998. PMID: 9765494 Free PMC article.

Genotypic subtypes of HIV-1 in Cameroon - PubMed (original) (raw)