Ectopic expression of NCAM in mouse fibroblasts stimulates self-aggregation, and promotes integration into primary cerebellum cell aggregates - PubMed (original) (raw)

Ectopic expression of NCAM in mouse fibroblasts stimulates self-aggregation, and promotes integration into primary cerebellum cell aggregates

U Hartmann et al. Cell Adhes Commun. 1994 Aug.

Abstract

We have undertaken aggregation experiments using mouse LMTK(-)-fibroblasts transfected with various isotypes of the neural cell adhesion molecule, NCAM. We found that self-aggregation of NCAM-positive fibroblasts is enhanced compared to control-transfected cells. The aggregation properties are partly dependent on the expressed NCAM isotype. Fibroblasts expressing a NCAM 140 isotype with exons a3 and pi were further tested in primary cerebellum cell re-aggregation experiments. While control-transfected fibroblasts could not be found in forming aggregates, fibroblasts ectopically expressing NCAM were integrated into neural cell aggregates. Time-lapse photography indicated that the nascent primary cell aggregates actively participated in the integration process by migration and attachment to nearby NCAM-positive fibroblasts.

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