Construction of a human full-length cDNA bank - PubMed (original) (raw)

Construction of a human full-length cDNA bank

S Kato et al. Gene. 1994.

Abstract

We aimed to construct a full-length cDNA bank from an entire set of human genes and to analyze the function of a protein encoded by each cDNA. To achieve this purpose, a multifunctional phagemid shuttle vector, pKA1, was constructed for preparing a high-quality cDNA library composed of full-length cDNA clones which can be sequenced and expressed in vitro and in mammalian cells without subcloning the cDNA fragment into other vectors. Using this as a vector primer, we have prepared a prototype of the bank composed of full-length cDNAs encoding 236 human proteins whose amino acid sequences are identical or similar to known proteins. Most cDNAs contain a putative cap site sequence, some of which show a pyrimidine-rich conserved sequence exhibiting polymorphism. It was confirmed that the vector permits efficient in vitro translation, expression in mammalian cells and the preparation of nested deletion mutants.

PubMed Disclaimer

Cited by

Novel method for the high-throughput production of phosphorylation site-specific monoclonal antibodies.
Kurosawa N, Wakata Y, Inobe T, Kitamura H, Yoshioka M, Matsuzawa S, Kishi Y, Isobe M. Kurosawa N, et al. Sci Rep. 2016 Apr 29;6:25174. doi: 10.1038/srep25174. Sci Rep. 2016. PMID: 27125496 Free PMC article.
Tumor-specific usage of alternative transcription start sites in colorectal cancer identified by genome-wide exon array analysis.
Thorsen K, Schepeler T, Øster B, Rasmussen MH, Vang S, Wang K, Hansen KQ, Lamy P, Pedersen JS, Eller A, Mansilla F, Laurila K, Wiuf C, Laurberg S, Dyrskjøt L, Ørntoft TF, Andersen CL. Thorsen K, et al. BMC Genomics. 2011 Oct 14;12:505. doi: 10.1186/1471-2164-12-505. BMC Genomics. 2011. PMID: 21999571 Free PMC article.
Incorporation of non-natural nucleotides into template-switching oligonucleotides reduces background and improves cDNA synthesis from very small RNA samples.
Kapteyn J, He R, McDowell ET, Gang DR. Kapteyn J, et al. BMC Genomics. 2010 Jul 2;11:413. doi: 10.1186/1471-2164-11-413. BMC Genomics. 2010. PMID: 20598146 Free PMC article.
In silico investigations on functional and haplotype tag SNPs associated with congenital long QT syndromes (LQTSs).
Sudandiradoss C, Sethumadhavan R. Sudandiradoss C, et al. Genomic Med. 2008 Dec;2(3-4):55-67. doi: 10.1007/s11568-009-9027-3. Epub 2009 Feb 12. Genomic Med. 2008. PMID: 19214780 Free PMC article.
Fine expression profiling of full-length transcripts using a size-unbiased cDNA library prepared with the vector-capping method.
Oshikawa M, Sugai Y, Usami R, Ohtoko K, Toyama S, Kato S. Oshikawa M, et al. DNA Res. 2008 Jun 30;15(3):123-36. doi: 10.1093/dnares/dsn010. Epub 2008 May 16. DNA Res. 2008. PMID: 18487259 Free PMC article.

Construction of a human full-length cDNA bank - PubMed (original) (raw)