Inversions produced during V(D)J rearrangement at IgH, the immunoglobulin heavy-chain locus - PubMed (original) (raw)

Inversions produced during V(D)J rearrangement at IgH, the immunoglobulin heavy-chain locus

A E Sollbach et al. Mol Cell Biol. 1995 Feb.

Abstract

Diversity in immunoglobulin antigen receptors is generated in part by V(D)J recombination. In this process, different combinations of gene elements are joined in various configurations. Products of V(D)J recombination are coding joints, signal joints, and hybrid junctions, which are generated by deletion or inversion. To determine their role in the generation of diversity, we have examined two sorts of recombination products, coding joints and hybrid junctions, that have formed by inversion at the mouse immunoglobulin heavy-chain locus. We developed a PCR assay for quantification and characterization of inverted rearrangements of DH and JH gene elements. In primary cells from adult mice, inverted DJH rearrangements are detectable but they are rare. There were approximately 1,100 to 2,200 inverted DJH coding joints and inverted DJH hybrid junctions in the marrow of one adult mouse femur. On day 16 of gestation, inverted DJH rearrangements are more abundant. There are approximately 20,000 inverted DJH coding joints and inverted DJH hybrid junctions per day 16 fetal liver. In fetal liver cells, the number of inverted DJH rearrangements remains relatively constant from day 14 to day 16 of gestation. Inverted DJH rearrangements to JH4, the most 3' JH element, are more frequently detected than inverted DJH rearrangements to other JH elements. We compare the frequencies of inverted DJH rearrangements to previously determined frequencies of uninverted DJH rearrangements (DJH rearrangements formed by deletion). We suggest that inverted DJH rearrangements are influenced by V(D)J recombination mechanistic constraints and cellular selection.

PubMed Disclaimer

Similar articles

• Novel rearrangements at the immunoglobulin D locus. Inversions and fusions add to IgH somatic diversity.
  Meek KD, Hasemann CA, Capra JD. Meek KD, et al. J Exp Med. 1989 Jul 1;170(1):39-57. doi: 10.1084/jem.170.1.39. J Exp Med. 1989. PMID: 2501448 Free PMC article.
• VHD rearrangements in human immunoglobulin heavy chain minilocus transgenic mice.
  Tuaillon N, Capra JD. Tuaillon N, et al. Eur J Immunol. 2000 Oct;30(10):2998-3005. doi: 10.1002/1521-4141(200010)30:10<2998::AID-IMMU2998>3.0.CO;2-X. Eur J Immunol. 2000. PMID: 11069083
• Regulation of immunoglobulin heavy-chain gene rearrangements.
  Chowdhury D, Sen R. Chowdhury D, et al. Immunol Rev. 2004 Aug;200:182-96. doi: 10.1111/j.0105-2896.2004.00177.x. Immunol Rev. 2004. PMID: 15242405 Review.
• Mechanism and control of V(D)J recombination at the immunoglobulin heavy chain locus.
  Jung D, Giallourakis C, Mostoslavsky R, Alt FW. Jung D, et al. Annu Rev Immunol. 2006;24:541-70. doi: 10.1146/annurev.immunol.23.021704.115830. Annu Rev Immunol. 2006. PMID: 16551259 Review.

Cited by

• Altered 3D chromatin structure permits inversional recombination at the IgH locus.
  Qiu X, Ma F, Zhao M, Cao Y, Shipp L, Liu A, Dutta A, Singh A, Braikia FZ, De S, Wood WH 3rd, Becker KG, Zhou W, Ji H, Zhao K, Atchison ML, Sen R. Qiu X, et al. Sci Adv. 2020 Aug 14;6(33):eaaz8850. doi: 10.1126/sciadv.aaz8850. eCollection 2020 Aug. Sci Adv. 2020. PMID: 32851160 Free PMC article.
• The fundamental role of chromatin loop extrusion in physiological V(D)J recombination.
  Zhang Y, Zhang X, Ba Z, Liang Z, Dring EW, Hu H, Lou J, Kyritsis N, Zurita J, Shamim MS, Presser Aiden A, Lieberman Aiden E, Alt FW. Zhang Y, et al. Nature. 2019 Sep;573(7775):600-604. doi: 10.1038/s41586-019-1547-y. Epub 2019 Sep 11. Nature. 2019. PMID: 31511698 Free PMC article.
• BRILIA: Integrated Tool for High-Throughput Annotation and Lineage Tree Assembly of B-Cell Repertoires.
  Lee DW, Khavrutskii IV, Wallqvist A, Bavari S, Cooper CL, Chaudhury S. Lee DW, et al. Front Immunol. 2017 Jan 17;7:681. doi: 10.3389/fimmu.2016.00681. eCollection 2016. Front Immunol. 2017. PMID: 28144239 Free PMC article.
• Two Mutually Exclusive Local Chromatin States Drive Efficient V(D)J Recombination.
  Bolland DJ, Koohy H, Wood AL, Matheson LS, Krueger F, Stubbington MJ, Baizan-Edge A, Chovanec P, Stubbs BA, Tabbada K, Andrews SR, Spivakov M, Corcoran AE. Bolland DJ, et al. Cell Rep. 2016 Jun 14;15(11):2475-87. doi: 10.1016/j.celrep.2016.05.020. Epub 2016 Jun 2. Cell Rep. 2016. PMID: 27264181 Free PMC article.
• Violation of the 12/23 rule of genomic V(D)J recombination is common in lymphocytes.
  Parkinson NJ, Roddis M, Ferneyhough B, Zhang G, Marsden AJ, Maslau S, Sanchez-Pearson Y, Barthlott T, Humphreys IR, Ladell K, Price DA, Ponting CP, Hollander G, Fischer MD. Parkinson NJ, et al. Genome Res. 2015 Feb;25(2):226-34. doi: 10.1101/gr.179770.114. Epub 2014 Nov 3. Genome Res. 2015. PMID: 25367293 Free PMC article.

References

1. 1. Proc Natl Acad Sci U S A. 1988 Mar;85(5):1581-5 - PubMed
2. 1. Cell. 1988 Dec 23;55(6):1099-107 - PubMed
3. 1. J Exp Med. 1989 Jul 1;170(1):39-57 - PubMed
4. 1. Eur J Immunol. 1989 Oct;19(10):1849-54 - PubMed
5. 1. Cell. 1989 Dec 1;59(5):859-70 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Taylor & Francis

• Molecular Biology Databases

  • Mouse Genome Informatics (MGI)