Enhancer traps in the Drosophila bithorax complex mark parasegmental domains - PubMed (original) (raw)
Comparative Study
Enhancer traps in the Drosophila bithorax complex mark parasegmental domains
K McCall et al. Genetics. 1994 Oct.
Abstract
Eight P elements carrying a beta-galactosidase (lacZ) reporter have been mapped to sites within the Drosophila bithorax complex. The bithorax complex contains three homeotic genes, and at least nine regulatory regions which control their expression in successive parasegments of the fly. The enhancer traps inserted at the promoter of one of the genes, Ultrabithorax, express lacZ in patterns which mimic the Ultrabithorax protein pattern. Enhancer traps in the regulatory regions do not mimic the endogenous genes, but express lacZ globally in the relevant parasegments. Some P elements carry large DNA fragments upstream of the lacZ promoter but internal to the P element. In cases where these internal sequences specify a lacZ pattern, that pattern is generally suppressed when the element is inserted in the bithorax complex. In embryos mutant for genes of the Polycomb group, the lacZ expression from the enhancer traps spreads to all segments. Thus, the enhancer traps reveal parasegmental domains that are maintained by Polycomb-mediated repression. Such domains may be realized by parasegmental differences in chromatin structure.
Similar articles
- Interactions of Drosophila Ultrabithorax regulatory regions with native and foreign promoters.
Casares F, Bender W, Merriam J, Sánchez-Herrero E. Casares F, et al. Genetics. 1997 Jan;145(1):123-37. doi: 10.1093/genetics/145.1.123. Genetics. 1997. PMID: 9017395 Free PMC article. - Functional reconstruction of trans regulation of the Ultrabithorax promoter by the products of two antagonistic genes, trithorax and Polycomb.
Chang YL, King BO, O'Connor M, Mazo A, Huang DH. Chang YL, et al. Mol Cell Biol. 1995 Dec;15(12):6601-12. doi: 10.1128/MCB.15.12.6601. Mol Cell Biol. 1995. PMID: 8524225 Free PMC article. - In situ dissection of the Fab-7 region of the bithorax complex into a chromatin domain boundary and a Polycomb-response element.
Mihaly J, Hogga I, Gausz J, Gyurkovics H, Karch F. Mihaly J, et al. Development. 1997 May;124(9):1809-20. doi: 10.1242/dev.124.9.1809. Development. 1997. PMID: 9165128 - Transcriptional silencing of homeotic genes in Drosophila.
Bienz M, Müller J. Bienz M, et al. Bioessays. 1995 Sep;17(9):775-84. doi: 10.1002/bies.950170907. Bioessays. 1995. PMID: 8763830 Review. - Unraveling cis-regulatory mechanisms at the abdominal-A and Abdominal-B genes in the Drosophila bithorax complex.
Akbari OS, Bousum A, Bae E, Drewell RA. Akbari OS, et al. Dev Biol. 2006 May 15;293(2):294-304. doi: 10.1016/j.ydbio.2006.02.015. Epub 2006 Mar 20. Dev Biol. 2006. PMID: 16545794 Review.
Cited by
- lncRNA read-through regulates the BX-C insulator Fub-1.
Ibragimov A, Bing XY, Shidlovskii YV, Levine M, Georgiev P, Schedl P. Ibragimov A, et al. Elife. 2023 Aug 10;12:e84711. doi: 10.7554/eLife.84711. Elife. 2023. PMID: 37643473 Free PMC article. - Redundant enhancers in the iab-5 domain cooperatively activate Abd-B in the A5 and A6 abdominal segments of Drosophila.
Postika N, Schedl P, Georgiev P, Kyrchanova O. Postika N, et al. Development. 2021 Oct 1;148(19):dev199827. doi: 10.1242/dev.199827. Epub 2021 Sep 24. Development. 2021. PMID: 34473267 Free PMC article. - Molecular Lessons from the Drosophila Bithorax Complex.
Bender WW. Bender WW. Genetics. 2020 Nov;216(3):613-617. doi: 10.1534/genetics.120.303708. Genetics. 2020. PMID: 33158983 Free PMC article. Review. - Complete reconstitution of bypass and blocking functions in a minimal artificial Fab-7 insulator from Drosophila bithorax complex.
Kyrchanova O, Sabirov M, Mogila V, Kurbidaeva A, Postika N, Maksimenko O, Schedl P, Georgiev P. Kyrchanova O, et al. Proc Natl Acad Sci U S A. 2019 Jul 2;116(27):13462-13467. doi: 10.1073/pnas.1907190116. Epub 2019 Jun 17. Proc Natl Acad Sci U S A. 2019. PMID: 31209019 Free PMC article. - Boundaries mediate long-distance interactions between enhancers and promoters in the Drosophila Bithorax complex.
Postika N, Metzler M, Affolter M, Müller M, Schedl P, Georgiev P, Kyrchanova O. Postika N, et al. PLoS Genet. 2018 Dec 12;14(12):e1007702. doi: 10.1371/journal.pgen.1007702. eCollection 2018 Dec. PLoS Genet. 2018. PMID: 30540750 Free PMC article.
References
- Trends Genet. 1990 Dec;6(12):416-21 - PubMed
- EMBO J. 1992 Oct;11(10):3653-61 - PubMed
- Development. 1992 Feb;114(2):493-505 - PubMed
- Dev Biol. 1993 Jul;158(1):131-44 - PubMed
- Science. 1991 Sep 6;253(5024):1110-7 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases