Proteolytic cleavage and cell wall anchoring at the LPXTG motif of surface proteins in gram-positive bacteria - PubMed (original) (raw)
Proteolytic cleavage and cell wall anchoring at the LPXTG motif of surface proteins in gram-positive bacteria
W W Navarre et al. Mol Microbiol. 1994 Oct.
Abstract
Many surface proteins are thought to be anchored to the cell wall of Gram-positive bacteria via their C-terminus. Cell wall anchoring requires a specific sorting signal, normally located at the predicted C-terminus of surface proteins. Here we show that when placed into the middle of a polypeptide chain, the sorting signal causes the specific cleavage of the precursor as well as the cell wall anchoring of its N-terminal fragment, while the C-terminal fragment remains within the cytoplasm. N-terminal sequencing of the C-terminal cleavage fragment suggests that the cleavage site is located between threonine (T) and glycine (G) of the LPXTG motif, the signature sequence of cell wall sorting signals. All surface proteins harbouring an LPXTG sequence motif may therefore be cleaved and anchored by a universal mechanism. We also propose a novel hypothesis for the cell wall linkage of surface proteins in Gram-positive bacteria.
Similar articles
- Anchor structure of cell wall surface proteins in Listeria monocytogenes.
Dhar G, Faull KF, Schneewind O. Dhar G, et al. Biochemistry. 2000 Apr 4;39(13):3725-33. doi: 10.1021/bi992347o. Biochemistry. 2000. PMID: 10736172 - Bacillus anthracis sortase A (SrtA) anchors LPXTG motif-containing surface proteins to the cell wall envelope.
Gaspar AH, Marraffini LA, Glass EM, Debord KL, Ton-That H, Schneewind O. Gaspar AH, et al. J Bacteriol. 2005 Jul;187(13):4646-55. doi: 10.1128/JB.187.13.4646-4655.2005. J Bacteriol. 2005. PMID: 15968076 Free PMC article. - Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall.
Mazmanian SK, Liu G, Ton-That H, Schneewind O. Mazmanian SK, et al. Science. 1999 Jul 30;285(5428):760-3. doi: 10.1126/science.285.5428.760. Science. 1999. PMID: 10427003 - Protein sorting to the cell wall envelope of Gram-positive bacteria.
Ton-That H, Marraffini LA, Schneewind O. Ton-That H, et al. Biochim Biophys Acta. 2004 Nov 11;1694(1-3):269-78. doi: 10.1016/j.bbamcr.2004.04.014. Biochim Biophys Acta. 2004. PMID: 15546671 Review. - Anchoring of LPXTG-Like Proteins to the Gram-Positive Cell Wall Envelope.
Siegel SD, Reardon ME, Ton-That H. Siegel SD, et al. Curr Top Microbiol Immunol. 2017;404:159-175. doi: 10.1007/82_2016_8. Curr Top Microbiol Immunol. 2017. PMID: 27097813 Review.
Cited by
- Bacterial surface-exposed lipoproteins and sortase-mediated anchored cell surface proteins in plant infection.
de Sandozequi A, Martínez-Anaya C. de Sandozequi A, et al. Microbiologyopen. 2023 Oct;12(5):e1382. doi: 10.1002/mbo3.1382. Microbiologyopen. 2023. PMID: 37877658 Free PMC article. Review. - Echinacoside, a promising sortase A inhibitor, combined with vancomycin against murine models of MRSA-induced pneumonia.
Jiang T, Yuan D, Wang R, Zhao C, Xu Y, Liu Y, Song W, Su X, Wang B. Jiang T, et al. Med Microbiol Immunol. 2023 Dec;212(6):421-435. doi: 10.1007/s00430-023-00782-9. Epub 2023 Oct 5. Med Microbiol Immunol. 2023. PMID: 37796314 - The NagY regulator: A member of the BglG/SacY antiterminator family conserved in Enterococcus faecalis and involved in virulence.
Soussan D, Salze M, Ledormand P, Sauvageot N, Boukerb A, Lesouhaitier O, Fichant G, Rincé A, Quentin Y, Muller C. Soussan D, et al. Front Microbiol. 2023 Feb 17;13:1070116. doi: 10.3389/fmicb.2022.1070116. eCollection 2022. Front Microbiol. 2023. PMID: 36875533 Free PMC article. - Bioinformatic analysis of WxL domain proteins.
Hassan MU, Williamson MP. Hassan MU, et al. Saudi J Biol Sci. 2023 Feb;30(2):103526. doi: 10.1016/j.sjbs.2022.103526. Epub 2022 Dec 7. Saudi J Biol Sci. 2023. PMID: 36568411 Free PMC article. - Biofilm Formation by Pathogenic Bacteria: Applying a Staphylococcus aureus Model to Appraise Potential Targets for Therapeutic Intervention.
Sedarat Z, Taylor-Robinson AW. Sedarat Z, et al. Pathogens. 2022 Mar 23;11(4):388. doi: 10.3390/pathogens11040388. Pathogens. 2022. PMID: 35456063 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases