Cytoplasmic juxtamembrane domain of the human EGF receptor is required for basolateral localization in MDCK cells - PubMed (original) (raw)
Cytoplasmic juxtamembrane domain of the human EGF receptor is required for basolateral localization in MDCK cells
M Hobert et al. J Cell Physiol. 1995 Mar.
Abstract
Although it is well established that epidermal growth factor receptors (EGFRs) are asymmetrically expressed at the basolateral plasma membrane in polarized epithelial cells, how this process is regulated is not known. The purpose of this study was to address the mechanism of directed EGFR basolateral sorting using the Madin-Darby canine kidney (MDCK) cell model. The first set of experiments established sorting patterns for endogenous canine EGFRs. The polarity of the canine EGFR was not quantitatively affected by differences in electrical resistance exhibited by the MDCK I and MDCK II cell strains. In both cases, greater than 90% of total surface EGFRs was localized to the basolateral surface. Canine EGFRs sort directly to the basolateral membrane from the trans-Golgi network with a half-time of approximately 45 min and have an approximate t1/2 of 12.5 h once reaching the basolateral surface. Human holoreceptors expressed in stably transfected MDCK cells also localize to the basolateral membrane with similar efficiency. To identify EGFR sequences necessary for basolateral sorting, MDCK cells were transfected with cDNAs coding for cytoplasmically truncated human receptor proteins. Human EGFRs truncated at Arg-651 were localized predominantly at the apical surface of filter-grown cells, whereas receptors truncated at Leu-723 were predominantly basolateral. These results suggest that the cytoplasmic juxtamembrane domain contains a positive basolateral sorting determinant. Moreover, the EGFR ectodomain or transmembrane domain may possess a cryptic sequence that specifically interacts with the apical sorting machinery once the dominant basolateral sorting signal is removed. Further elucidation of the precise location of these signals will enhance our basic understanding of regulated plasma membrane sorting, as well as the functional consequences of inappropriate EGFR expression associated with certain pathophysiologic and malignant states.
Similar articles
- Regulation of EGF signaling by cell polarity in MDCK kidney epithelial cells.
Hobert ME, Friend LA, Carlin CR. Hobert ME, et al. J Cell Physiol. 1999 Nov;181(2):330-41. doi: 10.1002/(SICI)1097-4652(199911)181:2<330::AID-JCP15>3.0.CO;2-S. J Cell Physiol. 1999. PMID: 10497312 - Polarized transport of MHC class II molecules in Madin-Darby canine kidney cells is directed by a leucine-based signal in the cytoplasmic tail of the beta-chain.
Simonsen A, Pedersen KW, Nordeng TW, von der Lippe A, Stang E, Long EO, Bakke O. Simonsen A, et al. J Immunol. 1999 Sep 1;163(5):2540-8. J Immunol. 1999. PMID: 10452991 - Sorting of H,K-ATPase beta-subunit in MDCK and LLC-PK cells is independent of mu 1B adaptin expression.
Duffield A, Fölsch H, Mellman I, Caplan MJ. Duffield A, et al. Traffic. 2004 Jun;5(6):449-61. doi: 10.1111/j.1398-9219.2004.00192.x. Traffic. 2004. PMID: 15117319 - Lipid polarity and sorting in epithelial cells.
van Meer G, Simons K. van Meer G, et al. J Cell Biochem. 1988 Jan;36(1):51-8. doi: 10.1002/jcb.240360106. J Cell Biochem. 1988. PMID: 3277985 Review. - Regulation of protein traffic in polarized epithelial cells.
Mostov KE. Mostov KE. Histol Histopathol. 1995 Apr;10(2):423-31. Histol Histopathol. 1995. PMID: 7599439 Review.
Cited by
- Loss of EGF receptor polarity enables homeostatic imbalance in epithelial-cell models.
Carlin CR, Ngalula S. Carlin CR, et al. Mol Biol Cell. 2023 Nov 1;34(12):ar116. doi: 10.1091/mbc.E23-04-0133. Epub 2023 Aug 30. Mol Biol Cell. 2023. PMID: 37647145 Free PMC article. - Induction of apically mistrafficked epiregulin disrupts epithelial polarity via aberrant EGFR signaling.
Singh B, Bogatcheva G, Krystofiak E, McKinley ET, Hill S, Rose KL, Higginbotham JN, Coffey RJ. Singh B, et al. J Cell Sci. 2021 Sep 15;134(18):jcs255927. doi: 10.1242/jcs.255927. Epub 2021 Sep 17. J Cell Sci. 2021. PMID: 34406412 Free PMC article. - The dynamics of morphogenesis in stem cell-based embryology: Novel insights for symmetry breaking.
Sozen B, Cornwall-Scoones J, Zernicka-Goetz M. Sozen B, et al. Dev Biol. 2021 Jun;474:82-90. doi: 10.1016/j.ydbio.2020.12.005. Epub 2020 Dec 15. Dev Biol. 2021. PMID: 33333067 Free PMC article. Review. - Proliferative signaling by ERBB proteins and RAF/MEK/ERK effectors in polycystic kidney disease.
Parker MI, Nikonova AS, Sun D, Golemis EA. Parker MI, et al. Cell Signal. 2020 Mar;67:109497. doi: 10.1016/j.cellsig.2019.109497. Epub 2019 Dec 9. Cell Signal. 2020. PMID: 31830556 Free PMC article. Review. - EGFR controls IQGAP basolateral membrane localization and mitotic spindle orientation during epithelial morphogenesis.
Bañón-Rodríguez I, Gálvez-Santisteban M, Vergarajauregui S, Bosch M, Borreguero-Pascual A, Martín-Belmonte F. Bañón-Rodríguez I, et al. EMBO J. 2014 Jan 13;33(2):129-45. doi: 10.1002/embj.201385946. EMBO J. 2014. PMID: 24421325 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous