Anatomical and functional imaging of neurons using 2-photon laser scanning microscopy - PubMed (original) (raw)
Review
Anatomical and functional imaging of neurons using 2-photon laser scanning microscopy
W Denk et al. J Neurosci Methods. 1994 Oct.
Abstract
Light scattering by brain tissue and phototoxicity are major obstacles to the use of high-resolution optical imaging and photo-activation ('uncaging') of bioactive compounds from inactive ('caged') precursors in intact and semi-intact nervous systems. Optical methods based on 2-photon excitation promise to reduce these obstacles (Denk, 1994; Denk et al., 1990, 1994). Here we show a range of imaging modes based on 2-photon laser scanning microscopy (TPLSM) as applicable to problems in neuroscience. Fluorescence images were taken of neurons labeled with ion-sensitive and voltage-sensitive dyes in invertebrate ganglia, mammalian brain slices, and from the intact mammalian brain. Scanning photochemical images with whole-cell current detection (Denk, 1994) show how the distribution of neurotransmitter receptors on the surface of specific cells can be mapped. All images show strong optical sectioning and usable images can be obtained at depths greater than 100 microns below the surface of the preparation.
Similar articles
- Two-photon imaging in living brain slices.
Mainen ZF, Maletic-Savatic M, Shi SH, Hayashi Y, Malinow R, Svoboda K. Mainen ZF, et al. Methods. 1999 Jun;18(2):231-9, 181. doi: 10.1006/meth.1999.0776. Methods. 1999. PMID: 10356355 - Intravital imaging of green fluorescent protein using two-photon laser-scanning microscopy.
Potter SM, Wang CM, Garrity PA, Fraser SE. Potter SM, et al. Gene. 1996;173(1 Spec No):25-31. doi: 10.1016/0378-1119(95)00681-8. Gene. 1996. PMID: 8707052 - Thinned-skull cranial window technique for long-term imaging of the cortex in live mice.
Yang G, Pan F, Parkhurst CN, Grutzendler J, Gan WB. Yang G, et al. Nat Protoc. 2010 Feb;5(2):201-8. doi: 10.1038/nprot.2009.222. Epub 2010 Jan 14. Nat Protoc. 2010. PMID: 20134419 Free PMC article. - Scanning laser photostimulation: a new approach for analyzing brain circuits.
Katz LC, Dalva MB. Katz LC, et al. J Neurosci Methods. 1994 Oct;54(2):205-18. doi: 10.1016/0165-0270(94)90194-5. J Neurosci Methods. 1994. PMID: 7869753 Review. - Fact and artefact in confocal microscopy.
Watson TF. Watson TF. Adv Dent Res. 1997 Nov;11(4):433-41. doi: 10.1177/08959374970110040901. Adv Dent Res. 1997. PMID: 9470501 Review.
Cited by
- High-pulse-energy multiphoton imaging of neurons and oligodendrocytes in deep murine brain with a fiber laser.
Redlich MJ, Prall B, Canto-Said E, Busarov Y, Shirinyan-Tuka L, Meah A, Lim H. Redlich MJ, et al. Sci Rep. 2021 Apr 12;11(1):7950. doi: 10.1038/s41598-021-86924-6. Sci Rep. 2021. PMID: 33846422 Free PMC article. - Imaging liver biology in vivo using conventional confocal microscopy.
Marques PE, Antunes MM, David BA, Pereira RV, Teixeira MM, Menezes GB. Marques PE, et al. Nat Protoc. 2015 Feb;10(2):258-68. doi: 10.1038/nprot.2015.006. Epub 2015 Jan 8. Nat Protoc. 2015. PMID: 25569332 - Two-photon calcium imaging of neuronal activity.
Grienberger C, Giovannucci A, Zeiger W, Portera-Cailliau C. Grienberger C, et al. Nat Rev Methods Primers. 2022;2(1):67. doi: 10.1038/s43586-022-00147-1. Epub 2022 Sep 1. Nat Rev Methods Primers. 2022. PMID: 38124998 Free PMC article. - Two-photon scanning microscopy of in vivo sensory responses of cortical neurons genetically encoded with a fluorescent voltage sensor in rat.
Ahrens KF, Heider B, Lee H, Isacoff EY, Siegel RM. Ahrens KF, et al. Front Neural Circuits. 2012 Mar 22;6:15. doi: 10.3389/fncir.2012.00015. eCollection 2012. Front Neural Circuits. 2012. PMID: 22461770 Free PMC article. - Next-Generation Tools to Study Autonomic Regulation In Vivo.
Mukerjee S, Lazartigues E. Mukerjee S, et al. Neurosci Bull. 2019 Feb;35(1):113-123. doi: 10.1007/s12264-018-0319-2. Epub 2018 Dec 17. Neurosci Bull. 2019. PMID: 30560436 Free PMC article. Review.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources