Antidromic identification of dopaminergic and other output neurons of the rat substantia nigra - PubMed (original) (raw)
Antidromic identification of dopaminergic and other output neurons of the rat substantia nigra
P G Guyenet et al. Brain Res. 1978.
Abstract
In the present study dopamine (DA)-containing and other output neurons of the substantia nigra (SN) wer identified by antidromic stimulation from postulated target nuclei, the caudate-putamen, the thalamus, the cortex and the pontine reticular formation. To guide electrode placements, the topography of the nigrostriatal projection system was determined by retrograde tracing methods. Spontaneously active cells present in the SN were then classified in two groups according to the shape of their action potentials and their firing rate. Type I cells were located mainly in the pars compacta and could be antidromically-activated (AD-activated) from various locations along the course of the nigrostriatal pathway (caudate-putamen, globus pallidus, MFB) but not from other brain areas (ventromedial thalamus, motor cortex, pontine reticular formation). These neurons had a slow bursting pattern of firing, a very slow conduction velocity (0.58 m/sec), and a wide action potential. Their firing rate was dramatically reduced following the intravenous administration of apomorphine (ID 50: 9.3 microgram/kg), or the iontophoretic application of DA and GABA. Type II cells were located predominantly in the pars reticulata; most of them could be AD-activated from the ventromedial thalamus and the MFB but not from the motor cortex. A few of these cells could be AD-activated from the pontine reticular formation and the thalamus. A minority of type II cells, located in or near the pars compacta could be AD-activated from the caudate-putamen. In addition, their conduction velocuty was much higher (2.8 m/sec) and their firing rate far in excess of that exhibited by type I neurons. These neurons were inhibited by the iontophoretic application of GABA but not of DA. The microinjection of 6-hydroxydopamine (a neurotoxin relatively specific against catecholamine-containing neurons) in the vicinity of the MFB blocked selectively the propagation of antidromic spikes in type I but not type II cells. It is concluded that type I cells are the DA neurons of the nigrostriatal pathway. Type II cells are mainly oupput neurons that project to the ventromedial thalamus, the pons and the forebrain. This telencephalic projection most likely constitutes a second, non-DA, fast-conducting nigrostriatal pathway.
Similar articles
- Non-dopaminergic nigrostriatal pathway.
Guyenet PG, Crane JK. Guyenet PG, et al. Brain Res. 1981 Jun 1;213(2):291-305. doi: 10.1016/0006-8993(81)90235-3. Brain Res. 1981. PMID: 7248760 - Electrophysiological evidence for branching nigral projections to pontine reticular formation, superior colliculus and thalamus.
Niijima K, Yoshida M. Niijima K, et al. Brain Res. 1982 May 6;239(1):279-82. doi: 10.1016/0006-8993(82)90852-6. Brain Res. 1982. PMID: 7093682 - GABAergic control of substantia nigra dopaminergic neurons.
Tepper JM, Lee CR. Tepper JM, et al. Prog Brain Res. 2007;160:189-208. doi: 10.1016/S0079-6123(06)60011-3. Prog Brain Res. 2007. PMID: 17499115 Review. - Transmitters for the afferent and efferent systems of the neostriatum and their possible interactions.
Kim JS. Kim JS. Adv Biochem Psychopharmacol. 1978;19:217-33. Adv Biochem Psychopharmacol. 1978. PMID: 29447 Review.
Cited by
- Orexin/hypocretin modulates response of ventral tegmental dopamine neurons to prefrontal activation: diurnal influences.
Moorman DE, Aston-Jones G. Moorman DE, et al. J Neurosci. 2010 Nov 17;30(46):15585-99. doi: 10.1523/JNEUROSCI.2871-10.2010. J Neurosci. 2010. PMID: 21084614 Free PMC article. - Electrophysiological and morphological evidence for a GABAergic nigrostriatal pathway.
Rodríguez M, González-Hernández T. Rodríguez M, et al. J Neurosci. 1999 Jun 1;19(11):4682-94. doi: 10.1523/JNEUROSCI.19-11-04682.1999. J Neurosci. 1999. PMID: 10341266 Free PMC article. - Dopamine and Beyond: Implications of Psychophysical Studies of Intracranial Self-Stimulation for the Treatment of Depression.
Pallikaras V, Shizgal P. Pallikaras V, et al. Brain Sci. 2022 Aug 8;12(8):1052. doi: 10.3390/brainsci12081052. Brain Sci. 2022. PMID: 36009115 Free PMC article. - Proceedings of the British Pharmacological Society. University of Leicester, 6th-8th July, 1983. Abstracts.
[No authors listed] [No authors listed] Br J Pharmacol. 1983 Sep;80 Suppl(Suppl):426P-594P. Br J Pharmacol. 1983. PMID: 6652382 Free PMC article. No abstract available. - Electrophysiological evidence for the existence of crossed nigrostriatal fibers.
Collingridge GL. Collingridge GL. Experientia. 1982 Jul 15;38(7):812-3. doi: 10.1007/BF01972287. Experientia. 1982. PMID: 7106249 No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous