Molecular cloning of a human genomic region containing the H blood group alpha(1,2)fucosyltransferase gene and two H locus-related DNA restriction fragments. Isolation of a candidate for the human Secretor blood group locus - PubMed (original) (raw)
. 1995 Mar 3;270(9):4632-9.
doi: 10.1074/jbc.270.9.4632.
Affiliations
- PMID: 7876234
- DOI: 10.1074/jbc.270.9.4632
Free article
Molecular cloning of a human genomic region containing the H blood group alpha(1,2)fucosyltransferase gene and two H locus-related DNA restriction fragments. Isolation of a candidate for the human Secretor blood group locus
S Rouquier et al. J Biol Chem. 1995.
Free article
Abstract
We have used the human H blood group alpha(1,2)fucosyltransferase (FUT1) cDNA to screen chromosome 19 cosmid libraries in a search for the human Secretor (Se) blood group gene (FUT2). One cosmid has been isolated that contains two distinct segments that cross-hybridize with FUT1. We have assembled a 100-kilobase (kb) cosmid contig, localized to 19q13.3, encompassing FUT1 and the two FUT1-related sequences, termed Sec1 and Sec2, for Secretor candidate 1 and 2. Sec1 and Sec2 are separated by 12 kb and are 65.5 kb and 35 kb apart, respectively, from the FUT1 gene. We used a cosmid-dependent direct cDNA selection method to clone a cDNA corresponding to a transcript that emanates from Sec2. This cDNA detects a 3.35-kb transcript in human tissues known to express the Se locus. Together with sequence and expression data reported in the accompanying article (Kelly, R. J., Rouquier, S., Giorgi, D., Lennon, G. G., and Lowe, J. B. (1995) J. Biol. Chem. 270, 4640-4649), these data demonstrate that Sec2 corresponds to the human Se blood group locus (FUT2). Our results furthermore define the physical relationship between the H and Se loci and confirm a hypothesis that these two loci represent distinct but closely linked alpha(1,2)fucosyltransferase genes.
Similar articles
- Molecular genetic analysis of the human Lewis histo-blood group system. II. Secretor gene inactivation by a novel single missense mutation A385T in Japanese nonsecretor individuals.
Kudo T, Iwasaki H, Nishihara S, Shinya N, Ando T, Narimatsu I, Narimatsu H. Kudo T, et al. J Biol Chem. 1996 Apr 19;271(16):9830-7. doi: 10.1074/jbc.271.16.9830. J Biol Chem. 1996. PMID: 8621666 - Molecular genetics of H, Se, Lewis and other fucosyltransferase genes.
Mollicone R, Cailleau A, Oriol R. Mollicone R, et al. Transfus Clin Biol. 1995;2(4):235-42. doi: 10.1016/s1246-7820(05)80089-8. Transfus Clin Biol. 1995. PMID: 8542021 Review. - Constructing chromosome- and region-specific cosmid maps of the human genome.
Carrano AV, de Jong PJ, Branscomb E, Slezak T, Watkins BW. Carrano AV, et al. Genome. 1989;31(2):1059-65. doi: 10.1139/g89-182. Genome. 1989. PMID: 2698823 Review.
Cited by
- A Pedigree Investigation of H-antigen Deletion Caused by Mutation of 658 C to T in the FUT1 Gene.
Li C, Gu Z, Hou Y, Gao Q, Xu G, Lu H. Li C, et al. Indian J Hematol Blood Transfus. 2024 Jul;40(3):504-507. doi: 10.1007/s12288-023-01669-8. Epub 2023 May 24. Indian J Hematol Blood Transfus. 2024. PMID: 39011245 - Associations Between ABO Blood Groups and Diseases in the Digestive System and Vein.
Jiang F, Liu Z, Zhang Y, Song T. Jiang F, et al. Int J Gen Med. 2024 Mar 27;17:1185-1191. doi: 10.2147/IJGM.S451087. eCollection 2024. Int J Gen Med. 2024. PMID: 38562208 Free PMC article. - FUT1 variants responsible for Bombay or para-Bombay phenotypes in a database.
Soejima M, Koda Y. Soejima M, et al. Sci Rep. 2023 Oct 14;13(1):17447. doi: 10.1038/s41598-023-44731-1. Sci Rep. 2023. PMID: 37838738 Free PMC article. - Detection of c.375A>G, c.385A>T, c.571C>T, and sedel2 of FUT2 via Real-Time PCR in a Single Tube.
Soejima M, Koda Y. Soejima M, et al. Diagnostics (Basel). 2023 Jun 10;13(12):2022. doi: 10.3390/diagnostics13122022. Diagnostics (Basel). 2023. PMID: 37370917 Free PMC article. - FUT2 inhibits the EMT and metastasis of colorectal cancer by increasing LRP1 fucosylation.
He L, Guo Z, Wang W, Tian S, Lin R. He L, et al. Cell Commun Signal. 2023 Mar 27;21(1):63. doi: 10.1186/s12964-023-01060-0. Cell Commun Signal. 2023. PMID: 36973740 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources