Differentiation state and invasiveness of human breast cancer cell lines - PubMed (original) (raw)

Comparative Study

doi: 10.1007/BF00666165.

Affiliations

• PMID: 7881109
• DOI: 10.1007/BF00666165

Comparative Study

Differentiation state and invasiveness of human breast cancer cell lines

C L Sommers et al. Breast Cancer Res Treat. 1994.

Abstract

Eighteen breast cancer cell lines were examined for expression of markers of epithelial and fibroblastic differentiation: E-cadherin, desmoplakins, ZO-1, vimentin, keratin and beta 1 and beta 4 integrins. The cell lines were distributed along a spectrum of differentiation from epithelial to fibroblastic phenotypes. The most well-differentiated, epithelioid cell lines contained proteins characteristic of desmosomal, adherens and tight junctions, were adherent to one another on plastic and in the basement membrane matrix Matrigel and were keratin-positive and vimentin-negative. These cell lines were all weakly invasive in an in vitro chemoinvasion assay. The most poorly-differentiated, fibroblastic cell lines were E-cadherin-, desmoplakin- and ZO-1-negative and formed branching structures in Matrigel. They were vimentin-positive, contained only low levels of keratins and were highly invasive in the in vitro chemoinvasion assay. Of all of the markers analyzed, vimentin expression correlated best with in vitro invasive ability and fibroblastic differentiation. In a cell line with unstable expression of vimentin, T47DCO, the cells that were invasive were of the fibroblastic type. The differentiation markers described here may be useful for analysis of clinical specimens and could potentially provide a more precise measure of differentiation grade yielding more power for predicting prognosis.

PubMed Disclaimer

Similar articles

• Loss of epithelial markers and acquisition of vimentin expression in adriamycin- and vinblastine-resistant human breast cancer cell lines.
  Sommers CL, Heckford SE, Skerker JM, Worland P, Torri JA, Thompson EW, Byers SW, Gelmann EP. Sommers CL, et al. Cancer Res. 1992 Oct 1;52(19):5190-7. Cancer Res. 1992. PMID: 1382837
• Cell adhesion molecule uvomorulin expression in human breast cancer cell lines: relationship to morphology and invasive capacities.
  Sommers CL, Thompson EW, Torri JA, Kemler R, Gelmann EP, Byers SW. Sommers CL, et al. Cell Growth Differ. 1991 Aug;2(8):365-72. Cell Growth Differ. 1991. PMID: 1793731
• Association of increased basement membrane invasiveness with absence of estrogen receptor and expression of vimentin in human breast cancer cell lines.
  Thompson EW, Paik S, Brünner N, Sommers CL, Zugmaier G, Clarke R, Shima TB, Torri J, Donahue S, Lippman ME, et al. Thompson EW, et al. J Cell Physiol. 1992 Mar;150(3):534-44. doi: 10.1002/jcp.1041500314. J Cell Physiol. 1992. PMID: 1537883
• Collagen induced MMP-2 activation in human breast cancer.
  Thompson EW, Yu M, Bueno J, Jin L, Maiti SN, Palao-Marco FL, Pulyaeva H, Tamborlane JW, Tirgari R, Wapnir I, et al. Thompson EW, et al. Breast Cancer Res Treat. 1994;31(2-3):357-70. doi: 10.1007/BF00666168. Breast Cancer Res Treat. 1994. PMID: 7881112 Review.

Cited by

• miR-564 acts as a dual inhibitor of PI3K and MAPK signaling networks and inhibits proliferation and invasion in breast cancer.
  Mutlu M, Saatci Ö, Ansari SA, Yurdusev E, Shehwana H, Konu Ö, Raza U, Şahin Ö. Mutlu M, et al. Sci Rep. 2016 Sep 7;6:32541. doi: 10.1038/srep32541. Sci Rep. 2016. PMID: 27600857 Free PMC article.
• Ecto-5'-nucleotidase promotes invasion, migration and adhesion of human breast cancer cells.
  Wang L, Zhou X, Zhou T, Ma D, Chen S, Zhi X, Yin L, Shao Z, Ou Z, Zhou P. Wang L, et al. J Cancer Res Clin Oncol. 2008 Mar;134(3):365-72. doi: 10.1007/s00432-007-0292-z. Epub 2007 Aug 2. J Cancer Res Clin Oncol. 2008. PMID: 17671792
• GLS2 is protumorigenic in breast cancers.
  Dias MM, Adamoski D, Dos Reis LM, Ascenção CFR, de Oliveira KRS, Mafra ACP, da Silva Bastos AC, Quintero M, de G Cassago C, Ferreira IM, Fidelis CHV, Rocco SA, Bajgelman MC, Stine Z, Berindan-Neagoe I, Calin GA, Ambrosio ALB, Dias SMG. Dias MM, et al. Oncogene. 2020 Jan;39(3):690-702. doi: 10.1038/s41388-019-1007-z. Epub 2019 Sep 20. Oncogene. 2020. PMID: 31541193
• Low Expression of Keratin17 is Related to Poor Prognosis in Bladder Cancer.
  Wu J, Xu H, Ji H, Zhai B, Zhu J, Gao M, Zhu H, Wang X. Wu J, et al. Onco Targets Ther. 2021 Jan 19;14:577-587. doi: 10.2147/OTT.S287891. eCollection 2021. Onco Targets Ther. 2021. PMID: 33500631 Free PMC article.
• Targeting triple-negative breast cancer cells with the histone deacetylase inhibitor panobinostat.
  Tate CR, Rhodes LV, Segar HC, Driver JL, Pounder FN, Burow ME, Collins-Burow BM. Tate CR, et al. Breast Cancer Res. 2012 May 21;14(3):R79. doi: 10.1186/bcr3192. Breast Cancer Res. 2012. PMID: 22613095 Free PMC article.

References

1. 1. Cancer Res. 1990 Sep 15;50(18):6087-94 - PubMed
2. 1. Cancer Res. 1991 May 1;51(9):2395-402 - PubMed
3. 1. Cell. 1991 Jul 12;66(1):107-19 - PubMed
4. 1. Annu Rev Biochem. 1990;59:237-52 - PubMed
5. 1. Anticancer Res. 1992 Nov-Dec;12(6B):1973-82 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Other Literature Sources

  • The Lens - Patent Citations Database

• Medical

  • MedlinePlus Health Information