Regulation of N-linked glycosylation. Neuronal cell-specific expression of a 5' extended transcript from the gene encoding N-acetylglucosaminyltransferase I - PubMed (original) (raw)
Regulation of N-linked glycosylation. Neuronal cell-specific expression of a 5' extended transcript from the gene encoding N-acetylglucosaminyltransferase I
J Yang et al. Glycobiology. 1994 Oct.
Erratum in
- Glycobiology 1995 May;5(3):279
Abstract
A key transferase in the generation of mature N-linked carbohydrates is the medial Golgi enzyme N-acetylglucosaminyltransferase I (EC 2.4.1.101; GlcNAc-TI) which is encoded by the Mgat-1 gene. Previous studies revealed two size classes of Mgat-1 mRNA that are differentially expressed in mouse tissues. Nearly all tissues possess a shorter form (approximately 2.9 kb), whereas brain has predominantly a longer Mgat-1 mRNA (approximately 3.3 kb). We now show, by in situ hybridization of horizontal sections of adult mouse brain, that Mgat-1 RNA levels vary markedly in different brain cell types with the greatest expression being in neuronal cells. The differential expression of the approximately 2.9 kb and approximately 3.3 kb Mgat-1 mRNAs is likely to be controlled by tissue-specific promoters since the size difference between the mRNAs was found to residue entirely in the 5' untranslated region of the approximately 3.3 kb mRNA. Evidence for this was obtained by an RNase H strategy, reverse transcription-polymerase chain reaction (RT-PCR) and 5' anchored PCR. All of the 0.4 kb difference in size was localized upstream of the previously isolated cDNA sequence. Sequence information from this region was obtained from a mouse brain cDNA library by a PCR amplification strategy and a probe specific for the 3.3 kb mRNA was generated. This probe hybridized uniquely to the approximately 3.3 kb Mgat-1 mRNA and Southern blot analysis showed that the new sequence is physically linked to the Mgat-1 gene. A tissue culture model which displays an increase in expression of the approximately 3.3 kb Mgat-1 mRNA transcript during differentiation to neuronal cells has been developed in P19 embryonal carcinoma (EC) cells. The combined data suggest that 5' exons in the Mgat-1 gene are differentially utilized by tissue-specific promoters and that transcription factor(s) which specify production of the approximately 3.3 kb Mgat-1 mRNA are induced by retinoic acid treatment of P19 EC cells.
Similar articles
- Organization of the human beta-1,2-N-acetylglucosaminyltransferase I gene (MGAT1), which controls complex and hybrid N-glycan synthesis.
Yip B, Chen SH, Mulder H, Höppener JW, Schachter H. Yip B, et al. Biochem J. 1997 Jan 15;321 ( Pt 2)(Pt 2):465-74. doi: 10.1042/bj3210465. Biochem J. 1997. PMID: 9020882 Free PMC article. - Cloning and expression of the murine gene and chromosomal location of the human gene encoding N-acetylglucosaminyltransferase I.
Kumar R, Yang J, Eddy RL, Byers MG, Shows TB, Stanley P. Kumar R, et al. Glycobiology. 1992 Aug;2(4):383-93. doi: 10.1093/glycob/2.4.383. Glycobiology. 1992. PMID: 1421759 - Expression of N-acetylglucosaminyltransferase V mRNA in mammalian tissues and cell lines.
Perng GS, Shoreibah M, Margitich I, Pierce M, Fregien N. Perng GS, et al. Glycobiology. 1994 Dec;4(6):867-71. doi: 10.1093/glycob/4.6.867. Glycobiology. 1994. PMID: 7734849 - Cloning and expression of N-acetylglucosaminyltransferase I, the medial Golgi transferase that initiates complex N-linked carbohydrate formation.
Kumar R, Yang J, Larsen RD, Stanley P. Kumar R, et al. Proc Natl Acad Sci U S A. 1990 Dec;87(24):9948-52. doi: 10.1073/pnas.87.24.9948. Proc Natl Acad Sci U S A. 1990. PMID: 1702225 Free PMC article.
Cited by
- The joys of HexNAc. The synthesis and function of N- and O-glycan branches.
Schachter H. Schachter H. Glycoconj J. 2000 Jul-Sep;17(7-9):465-83. doi: 10.1023/a:1011010206774. Glycoconj J. 2000. PMID: 11421343 Review. - Basolateral sorting signals regulating tissue-specific polarity of heteromeric monocarboxylate transporters in epithelia.
Castorino JJ, Deborde S, Deora A, Schreiner R, Gallagher-Colombo SM, Rodriguez-Boulan E, Philp NJ. Castorino JJ, et al. Traffic. 2011 Apr;12(4):483-98. doi: 10.1111/j.1600-0854.2010.01155.x. Epub 2011 Feb 1. Traffic. 2011. PMID: 21199217 Free PMC article. - Pore positioning: current concepts in Pannexin channel trafficking.
Boyce AK, Prager RT, Wicki-Stordeur LE, Swayne LA. Boyce AK, et al. Channels (Austin). 2014;8(2):110-7. doi: 10.4161/chan.27287. Epub 2013 Dec 3. Channels (Austin). 2014. PMID: 24300303 Free PMC article. Review. - Organization of the human beta-1,2-N-acetylglucosaminyltransferase I gene (MGAT1), which controls complex and hybrid N-glycan synthesis.
Yip B, Chen SH, Mulder H, Höppener JW, Schachter H. Yip B, et al. Biochem J. 1997 Jan 15;321 ( Pt 2)(Pt 2):465-74. doi: 10.1042/bj3210465. Biochem J. 1997. PMID: 9020882 Free PMC article. - Regulation of UDP-N-acetylglucosamine:dolichyl-phosphate N-acetylglucosamine-1-phosphate transferase by retinoic acid in P19 cells.
Meissner JD, Naumann A, Mueller WH, Scheibe RJ. Meissner JD, et al. Biochem J. 1999 Mar 1;338 ( Pt 2)(Pt 2):561-8. Biochem J. 1999. PMID: 10024536 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases