Cloning and characterisation of the Schizosaccharomyces pombe rad32 gene: a gene required for repair of double strand breaks and recombination - PubMed (original) (raw)
Cloning and characterisation of the Schizosaccharomyces pombe rad32 gene: a gene required for repair of double strand breaks and recombination
M Tavassoli et al. Nucleic Acids Res. 1995.
Free PMC article
Abstract
A new Schizosaccharomyces pombe mutant (rad32) which is sensitive to gamma and UV irradiation is described. Pulsed field gel electrophoresis of DNA from irradiated cells indicates that the rad32 mutant, in comparison to wild type cells, has decreased ability to repair DNA double strand breaks. The mutant also undergoes decreased meiotic recombination and displays reduced stability of minichromosomes. The rad32 gene has been cloned by complementation of the UV sensitive phenotype. The gene, which is not essential for cell viability and is expressed at a moderate level in mitotically dividing cells, has significant homology to the meiotic recombination gene MRE11 of Saccharomyces cerevisiae. Epistasis analysis indicates that rad32 functions in a pathway which includes the rhp51 gene (the S.pombe homologue to S.cerevisiae RAD51) and that cells deleted for the rad32 gene in conjunction with either the rad3 deletion (a G2 checkpoint mutation) or the rad2 deletion (a chromosome stability and potential nucleotide excision repair mutation) are not viable.
Similar articles
- Schizosaccharomyces pombe rad32 protein: a phosphoprotein with an essential phosphoesterase motif required for repair of DNA double strand breaks.
Wilson S, Tavassoli M, Watts FZ. Wilson S, et al. Nucleic Acids Res. 1998 Dec 1;26(23):5261-9. doi: 10.1093/nar/26.23.5261. Nucleic Acids Res. 1998. PMID: 9826747 Free PMC article. - The role of Schizosaccharomyces pombe Rad32, the Mre11 homologue, and other DNA damage response proteins in non-homologous end joining and telomere length maintenance.
Wilson S, Warr N, Taylor DL, Watts FZ. Wilson S, et al. Nucleic Acids Res. 1999 Jul 1;27(13):2655-61. doi: 10.1093/nar/27.13.2655. Nucleic Acids Res. 1999. PMID: 10373582 Free PMC article. - A new recombinational DNA repair gene from Schizosaccharomyces pombe with homology to Escherichia coli RecA.
Khasanov FK, Savchenko GV, Bashkirova EV, Korolev VG, Heyer WD, Bashkirov VI. Khasanov FK, et al. Genetics. 1999 Aug;152(4):1557-72. doi: 10.1093/genetics/152.4.1557. Genetics. 1999. PMID: 10430583 Free PMC article. - DNA repair in the fission yeast, Schizosaccharomyces pombe.
Lehmann AR, Carr AM, Watts FZ, Murray JM. Lehmann AR, et al. Mutat Res. 1991 Sep-Oct;250(1-2):205-10. doi: 10.1016/0027-5107(91)90177-p. Mutat Res. 1991. PMID: 1944337 Review. - Repair of UV damage in the fission yeast Schizosaccharomyces pombe.
McCready SJ, Osman1 F, Yasui A. McCready SJ, et al. Mutat Res. 2000 Jun 30;451(1-2):197-210. doi: 10.1016/s0027-5107(00)00050-6. Mutat Res. 2000. PMID: 10915873 Review.
Cited by
- Divergence and conservation of the meiotic recombination machinery.
Arter M, Keeney S. Arter M, et al. Nat Rev Genet. 2024 May;25(5):309-325. doi: 10.1038/s41576-023-00669-8. Epub 2023 Nov 30. Nat Rev Genet. 2024. PMID: 38036793 Review. - OsMre11 Is Required for Mitosis during Rice Growth and Development.
Shen M, Nie Y, Chen Y, Zhang X, Zhao J. Shen M, et al. Int J Mol Sci. 2020 Dec 26;22(1):169. doi: 10.3390/ijms22010169. Int J Mol Sci. 2020. PMID: 33375295 Free PMC article. - Ctp1-dependent clipping and resection of DNA double-strand breaks by Mre11 endonuclease complex are not genetically separable.
Jensen KL, Russell P. Jensen KL, et al. Nucleic Acids Res. 2016 Sep 30;44(17):8241-9. doi: 10.1093/nar/gkw557. Epub 2016 Jun 20. Nucleic Acids Res. 2016. PMID: 27325741 Free PMC article. - Coordination of DNA damage tolerance mechanisms with cell cycle progression in fission yeast.
Callegari AJ, Kelly TJ. Callegari AJ, et al. Cell Cycle. 2016;15(2):261-73. doi: 10.1080/15384101.2015.1121353. Cell Cycle. 2016. PMID: 26652183 Free PMC article. - MRE11 is required for homologous synapsis and DSB processing in rice meiosis.
Ji J, Tang D, Wang M, Li Y, Zhang L, Wang K, Li M, Cheng Z. Ji J, et al. Chromosoma. 2013 Oct;122(5):363-76. doi: 10.1007/s00412-013-0421-1. Epub 2013 Jun 22. Chromosoma. 2013. PMID: 23793712
References
- Adv Genet. 1985;23:1-72 - PubMed
- J Cell Sci. 1994 Mar;107 ( Pt 3):601-13 - PubMed
- Mol Gen Genet. 1989 Sep;218(3):554-8 - PubMed
- Cell. 1992 May 1;69(3):457-70 - PubMed
- Nat Genet. 1993 Jul;4(3):239-43 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous