Detection of methanotrophic bacteria in environmental samples with the PCR - PubMed (original) (raw)
Detection of methanotrophic bacteria in environmental samples with the PCR
I R McDonald et al. Appl Environ Microbiol. 1995 Jan.
Abstract
We designed PCR primers by using the DNA sequences of the soluble methane monooxygenase gene clusters of Methylosinus trichosporium OB3b and Methylococcus capsulatus (Bath), and these primers were found to be specific for four of the five structural genes in the soluble methane monooxygenase gene clusters of several methanotrophs. We also designed primers for the gram-negative methylotroph-specific methanol dehydrogenase gene moxF. The specificity of these primers was confirmed by hybridizing and sequencing the PCR products obtained. The primers were then used to amplify methanotroph DNAs in samples obtained from various aquatic and terrestrial environments. Our sequencing data suggest that a large number of different methanotrophs are present in peat samples and also that there is a high level of variability in the mmoC gene, which codes for the reductase component of the soluble methane monooxygenase, while the mmoX gene, which codes for the alpha subunit of the hydroxylase component of this enzyme complex, appears to be highly conserved in methanotrophs.
Similar articles
- Detection of soluble methane monooxygenase producing Methylosinus trichosporium OB3b by polymerase chain reaction.
Mohan KS, Walia SK. Mohan KS, et al. Can J Microbiol. 1994 Nov;40(11):969-73. doi: 10.1139/m94-154. Can J Microbiol. 1994. PMID: 7804907 - Improved method for detection of methanotrophic bacteria in forest soils by PCR.
Steinkamp R, Zimmer W, Papen H. Steinkamp R, et al. Curr Microbiol. 2001 May;42(5):316-22. doi: 10.1007/s002840010223. Curr Microbiol. 2001. PMID: 11400051 - Soluble methane monooxygenase component B gene probe for identification of methanotrophs that rapidly degrade trichloroethylene.
Tsien HC, Hanson RS. Tsien HC, et al. Appl Environ Microbiol. 1992 Mar;58(3):953-60. doi: 10.1128/aem.58.3.953-960.1992. Appl Environ Microbiol. 1992. PMID: 1349468 Free PMC article. - Molecular genetics of methane oxidation.
Murrell JC. Murrell JC. Biodegradation. 1994 Dec;5(3-4):145-59. doi: 10.1007/BF00696456. Biodegradation. 1994. PMID: 7765830 Review. - Methanotrophs, Methylosinus trichosporium OB3b, sMMO, and their application to bioremediation.
Sullivan JP, Dickinson D, Chase HA. Sullivan JP, et al. Crit Rev Microbiol. 1998;24(4):335-73. doi: 10.1080/10408419891294217. Crit Rev Microbiol. 1998. PMID: 9887367 Review.
Cited by
- Thermophilic methane oxidation is widespread in Aotearoa-New Zealand geothermal fields.
Houghton KM, Carere CR, Stott MB, McDonald IR. Houghton KM, et al. Front Microbiol. 2023 Aug 31;14:1253773. doi: 10.3389/fmicb.2023.1253773. eCollection 2023. Front Microbiol. 2023. PMID: 37720161 Free PMC article. - Isolation of Methane Enriched Bacterial Communities and Application as Wheat Biofertilizer under Drought Conditions: An Environmental Contribution.
Barros-Rodríguez A, García-Gálvez C, Pacheco P, Kalyuzhnaya MG, Manzanera M. Barros-Rodríguez A, et al. Plants (Basel). 2023 Jun 29;12(13):2487. doi: 10.3390/plants12132487. Plants (Basel). 2023. PMID: 37447048 Free PMC article. - Molecular Ecology of Isoprene-Degrading Bacteria.
Carrión O, McGenity TJ, Murrell JC. Carrión O, et al. Microorganisms. 2020 Jun 27;8(7):967. doi: 10.3390/microorganisms8070967. Microorganisms. 2020. PMID: 32605141 Free PMC article. Review. - Gene probing reveals the widespread distribution, diversity and abundance of isoprene-degrading bacteria in the environment.
Carrión O, Larke-Mejía NL, Gibson L, Farhan Ul Haque M, Ramiro-García J, McGenity TJ, Murrell JC. Carrión O, et al. Microbiome. 2018 Dec 7;6(1):219. doi: 10.1186/s40168-018-0607-0. Microbiome. 2018. PMID: 30526688 Free PMC article. - Probiotic Properties of Exopolysaccharide-Producing Lactobacillus Strains Isolated from Tempoyak.
Khalil ES, Abd Manap MY, Mustafa S, Alhelli AM, Shokryazdan P. Khalil ES, et al. Molecules. 2018 Feb 13;23(2):398. doi: 10.3390/molecules23020398. Molecules. 2018. PMID: 29438288 Free PMC article.
References
- Appl Environ Microbiol. 1990 Feb;56(2):307-14 - PubMed
- Appl Environ Microbiol. 1992 Mar;58(3):953-60 - PubMed
- Appl Environ Microbiol. 1992 Sep;58(9):2717-22 - PubMed
- Appl Environ Microbiol. 1990 Mar;56(3):782-7 - PubMed
- Appl Environ Microbiol. 1993 Feb;59(2):353-7 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources