Phosphorylation of rabphilin-3A by Ca2+/calmodulin- and cAMP-dependent protein kinases in vitro - PubMed (original) (raw)
Comparative Study
Phosphorylation of rabphilin-3A by Ca2+/calmodulin- and cAMP-dependent protein kinases in vitro
E M Fykse et al. J Neurosci. 1995 Mar.
Abstract
Regulation of neurotransmitter release is thought to involve modulation of the release probability by protein phosphorylation. In order to identify novel targets for such regulatory processes, we have studied the phosphorylation of rabphilin-3A in vitro. Rabphilin-3A is a synaptic vesicle protein that interacts with rab3A in a GTP-dependent manner and binds Ca2+ in a phospholipid-dependent manner. Here we show that rabphilin-3A is an efficient substrate for Ca2+/calmodulin-dependent protein kinase II, which phosphorylates rat rabphilin-3A at residue 234 and 274, and for cAMP-dependent protein kinase, which phosphorylates rat rabphilin-3A at residue 234. This identifies the middle region of rabphilin-3A situated between the N-terminal rab3A-binding sequences and the C-terminal C2-domains involved in Ca2+/phospholipid binding as a regulatory domain. Thus, rabphilin-3A is a second phosphoprotein on synaptic vesicles that, similar to synapsin I, may integrate phosphorylation signals from multiple protein kinase signaling pathways in the cell.
Similar articles
- Phosphorylation of Rabphilin-3A by calmodulin-dependent protein kinase II.
Kato M, Sasaki T, Imazumi K, Takahashi K, Araki K, Shirataki H, Matsuura Y, Ishida A, Fujisawa H, Takai Y. Kato M, et al. Biochem Biophys Res Commun. 1994 Dec 30;205(3):1776-84. doi: 10.1006/bbrc.1994.2875. Biochem Biophys Res Commun. 1994. PMID: 7811264 - Phosphorylation of Rabphilin-3A, a putative target protein for Rab3A, by cyclic AMP-dependent protein kinase.
Numata S, Shirataki H, Hagi S, Yamamoto T, Takai Y. Numata S, et al. Biochem Biophys Res Commun. 1994 Sep 30;203(3):1927-34. doi: 10.1006/bbrc.1994.2413. Biochem Biophys Res Commun. 1994. PMID: 7945346 - Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid-binding protein, depends on rab3A/3C.
Li C, Takei K, Geppert M, Daniell L, Stenius K, Chapman ER, Jahn R, De Camilli P, Südhof TC. Li C, et al. Neuron. 1994 Oct;13(4):885-98. doi: 10.1016/0896-6273(94)90254-2. Neuron. 1994. PMID: 7946335 - Rab3A-rabphilin-3A system in neurotransmitter release.
Sasaki T, Shirataki H, Nakanishi H, Takai Y. Sasaki T, et al. Adv Second Messenger Phosphoprotein Res. 1997;31:279-94. doi: 10.1016/s1040-7952(97)80025-0. Adv Second Messenger Phosphoprotein Res. 1997. PMID: 9344258 Review. No abstract available. - Small Rab GTPases in Intracellular Vesicle Trafficking: The Case of Rab3A/Raphillin-3A Complex in the Kidney.
Martinez-Arroyo O, Selma-Soriano E, Ortega A, Cortes R, Redon J. Martinez-Arroyo O, et al. Int J Mol Sci. 2021 Jul 18;22(14):7679. doi: 10.3390/ijms22147679. Int J Mol Sci. 2021. PMID: 34299299 Free PMC article. Review.
Cited by
- Kinesin- and myosin-driven steps of vesicle recruitment for Ca2+-regulated exocytosis.
Bi GQ, Morris RL, Liao G, Alderton JM, Scholey JM, Steinhardt RA. Bi GQ, et al. J Cell Biol. 1997 Sep 8;138(5):999-1008. doi: 10.1083/jcb.138.5.999. J Cell Biol. 1997. PMID: 9281579 Free PMC article. - Rab3 reversibly recruits rabphilin to synaptic vesicles by a mechanism analogous to raf recruitment by ras.
Stahl B, Chou JH, Li C, Südhof TC, Jahn R. Stahl B, et al. EMBO J. 1996 Apr 15;15(8):1799-809. EMBO J. 1996. PMID: 8617225 Free PMC article. - Phosphorylation of synaptic vesicle proteins: modulation of the alpha SNAP interaction with the core complex.
Hirling H, Scheller RH. Hirling H, et al. Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11945-9. doi: 10.1073/pnas.93.21.11945. Proc Natl Acad Sci U S A. 1996. PMID: 8876242 Free PMC article. - Regulation of neuronal plasticity in the central nervous system by phosphorylation and dephosphorylation.
Tokuda M, Hatase O. Tokuda M, et al. Mol Neurobiol. 1998 Winter;17(1-3):137-56. doi: 10.1007/BF02802028. Mol Neurobiol. 1998. PMID: 9887450 Review. - Aging, synaptic dysfunction, and insulin-like growth factor (IGF)-1.
Deak F, Sonntag WE. Deak F, et al. J Gerontol A Biol Sci Med Sci. 2012 Jun;67(6):611-25. doi: 10.1093/gerona/gls118. Epub 2012 Apr 12. J Gerontol A Biol Sci Med Sci. 2012. PMID: 22503992 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous