The DNA binding domains of the yeast Gal4 and human c-Jun transcription factors interact through the zinc-finger and bZIP motifs - PubMed (original) (raw)
The DNA binding domains of the yeast Gal4 and human c-Jun transcription factors interact through the zinc-finger and bZIP motifs
K Sollerbrant et al. Nucleic Acids Res. 1995.
Free PMC article
Abstract
Different Gal4 fusion proteins, expressing unrelated transcription activator domains, were found to activate transcription from promoters containing dimerized AP1 DNA binding sites. Transactivation was dependent on the first 74 amino acids of Gal4. A direct interaction between Gal4 and c-Jun was demonstrated using a GSTGal4 fusion protein and in vitro translated human c-Jun. The interaction required the zinc finger containing DNA binding domain of Gal4 and the basic-leucine zipper region of c-Jun. These results demonstrated that the specificity of Gal4 fusion proteins in transient transfection experiments in mammalian cells is not restricted to reporters containing Gal4 binding sites, but also includes promoters containing AP1 binding sites. Furthermore, the Gal4 fusion proteins also activated transcription from a pUC18 vector fragment containing several putative AP1 binding sites. Finally, our results indicate that Gal4 activator proteins binding to Gal4 binding sites and to DNA bound AP1 factors can co-operatively activate transcription.
Similar articles
- Functional domains of the heavy metal-responsive transcription regulator MTF-1.
Radtke F, Georgiev O, Müller HP, Brugnera E, Schaffner W. Radtke F, et al. Nucleic Acids Res. 1995 Jun 25;23(12):2277-86. doi: 10.1093/nar/23.12.2277. Nucleic Acids Res. 1995. PMID: 7610056 Free PMC article. - Structure of the DNA-binding domain of zinc GAL4.
Kraulis PJ, Raine AR, Gadhavi PL, Laue ED. Kraulis PJ, et al. Nature. 1992 Apr 2;356(6368):448-50. doi: 10.1038/356448a0. Nature. 1992. PMID: 1557129 - A dual role for zinc fingers in both DNA binding and zinc sensing by the Zap1 transcriptional activator.
Bird AJ, Zhao H, Luo H, Jensen LT, Srinivasan C, Evans-Galea M, Winge DR, Eide DJ. Bird AJ, et al. EMBO J. 2000 Jul 17;19(14):3704-13. doi: 10.1093/emboj/19.14.3704. EMBO J. 2000. PMID: 10899124 Free PMC article. - Custom DNA-binding proteins come of age: polydactyl zinc-finger proteins.
Segal DJ, Barbas CF 3rd. Segal DJ, et al. Curr Opin Biotechnol. 2001 Dec;12(6):632-7. doi: 10.1016/s0958-1669(01)00272-5. Curr Opin Biotechnol. 2001. PMID: 11849947 Review. - The activation and activity control of AP-1 (fos/jun).
Radler-Pohl A, Gebel S, Sachsenmaier C, König H, Krämer M, Oehler T, Streile M, Ponta H, Rapp U, Rahmsdorf HJ, et al. Radler-Pohl A, et al. Ann N Y Acad Sci. 1993 Jun 11;684:127-48. doi: 10.1111/j.1749-6632.1993.tb32277.x. Ann N Y Acad Sci. 1993. PMID: 8317826 Review. No abstract available.
Cited by
- Mapping of a yeast G protein betagamma signaling interaction.
Dowell SJ, Bishop AL, Dyos SL, Brown AJ, Whiteway MS. Dowell SJ, et al. Genetics. 1998 Dec;150(4):1407-17. doi: 10.1093/genetics/150.4.1407. Genetics. 1998. PMID: 9832519 Free PMC article. - The leucine domain of the visna virus Tat protein mediates targeting to an AP-1 site in the viral long terminal repeat.
Carruth LM, Morse BA, Clements JE. Carruth LM, et al. J Virol. 1996 Jul;70(7):4338-44. doi: 10.1128/JVI.70.7.4338-4344.1996. J Virol. 1996. PMID: 8676456 Free PMC article. - GATA transcription factors associate with a novel class of nuclear bodies in erythroblasts and megakaryocytes.
Elefanty AG, Antoniou M, Custodio N, Carmo-Fonseca M, Grosveld FG. Elefanty AG, et al. EMBO J. 1996 Jan 15;15(2):319-33. EMBO J. 1996. PMID: 8617207 Free PMC article.
References
- Cell. 1978 Jul;14(3):725-31 - PubMed
- Mol Cell Biol. 1993 May;13(5):3042-9 - PubMed
- Cell. 1987 Jun 19;49(6):729-39 - PubMed
- Science. 1987 Dec 4;238(4832):1386-92 - PubMed
- Science. 1988 Jun 24;240(4860):1759-64 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous