The effect on synaptic physiology of synaptotagmin mutations in Drosophila - PubMed (original) (raw)
The effect on synaptic physiology of synaptotagmin mutations in Drosophila
A DiAntonio et al. Neuron. 1994 Apr.
Abstract
Synaptotagmin is a synaptic vesicle protein implicated in neurotransmitter release. Molecular characterization of four mutant alleles of this protein in Drosophila melanogaster has permitted an investigation of synaptotagmin's role in synaptic physiology and of some of the structural requirements for its function. Reduced levels of synaptotagmin resulted in a substantial alteration in synaptic function in the eye and at larval neuromuscular junctions. Decreased neurotransmitter release caused smaller evoked synaptic potentials. However, the frequency, but not the size, of spontaneous quantal events was simultaneously increased. These abnormalities do not appear to be secondary to a detectable morphological change in the arborization of the synapse. The increased frequency of spontaneous events was insufficient to deplete significantly the vesicle supply and thereby account for reduced transmission. These data are discussed in the context of models in which synaptotagmin's function includes a role in vesicle docking.
Similar articles
- Calcium dependence of neurotransmitter release and rate of spontaneous vesicle fusions are altered in Drosophila synaptotagmin mutants.
Littleton JT, Stern M, Perin M, Bellen HJ. Littleton JT, et al. Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):10888-92. doi: 10.1073/pnas.91.23.10888. Proc Natl Acad Sci U S A. 1994. PMID: 7971978 Free PMC article. - Roles of SNARE proteins and synaptotagmin I in synaptic transmission: studies at the Drosophila neuromuscular synapse.
Kidokoro Y. Kidokoro Y. Neurosignals. 2003 Jan-Feb;12(1):13-30. doi: 10.1159/000068912. Neurosignals. 2003. PMID: 12624525 Review. - Expression of synaptotagmin in Drosophila reveals transport and localization of synaptic vesicles to the synapse.
Littleton JT, Bellen HJ, Perin MS. Littleton JT, et al. Development. 1993 Aug;118(4):1077-88. doi: 10.1242/dev.118.4.1077. Development. 1993. PMID: 8269841 - The C(2)B Ca(2+)-binding motif of synaptotagmin is required for synaptic transmission in vivo.
Mackler JM, Drummond JA, Loewen CA, Robinson IM, Reist NE. Mackler JM, et al. Nature. 2002 Jul 18;418(6895):340-4. doi: 10.1038/nature00846. Epub 2002 Jul 7. Nature. 2002. PMID: 12110842 - Synaptotagmin controls and modulates synaptic-vesicle fusion in a Ca(2+)-dependent manner.
Littleton JT, Bellen HJ. Littleton JT, et al. Trends Neurosci. 1995 Apr;18(4):177-83. doi: 10.1016/0166-2236(95)93898-8. Trends Neurosci. 1995. PMID: 7778189 Review.
Cited by
- Mutations of Single Residues in the Complexin N-terminus Exhibit Distinct Phenotypes in Synaptic Vesicle Fusion.
Toulme E, Murach J, Bärfuss S, Kroll J, Malsam J, Trimbuch T, Herman MA, Söllner TH, Rosenmund C. Toulme E, et al. J Neurosci. 2024 Jul 31;44(31):e0076242024. doi: 10.1523/JNEUROSCI.0076-24.2024. J Neurosci. 2024. PMID: 38951039 - Mechanisms controlling the trafficking, localization, and abundance of presynaptic Ca2+ channels.
Cunningham KL, Littleton JT. Cunningham KL, et al. Front Mol Neurosci. 2023 Jan 13;15:1116729. doi: 10.3389/fnmol.2022.1116729. eCollection 2022. Front Mol Neurosci. 2023. PMID: 36710932 Free PMC article. Review. - The Core Complex of the Ca2+-Triggered Presynaptic Fusion Machinery.
Brunger AT, Leitz J. Brunger AT, et al. J Mol Biol. 2023 Jan 15;435(1):167853. doi: 10.1016/j.jmb.2022.167853. Epub 2022 Oct 13. J Mol Biol. 2023. PMID: 36243149 Free PMC article. Review. - Deconstructing Synaptotagmin-1's Distinct Roles in Synaptic Vesicle Priming and Neurotransmitter Release.
Bouazza-Arostegui B, Camacho M, Brockmann MM, Zobel S, Rosenmund C. Bouazza-Arostegui B, et al. J Neurosci. 2022 Apr 6;42(14):2856-2871. doi: 10.1523/JNEUROSCI.1945-21.2022. Epub 2022 Feb 22. J Neurosci. 2022. PMID: 35193927 Free PMC article. - The decoy SNARE Tomosyn sets tonic versus phasic release properties and is required for homeostatic synaptic plasticity.
Sauvola CW, Akbergenova Y, Cunningham KL, Aponte-Santiago NA, Littleton JT. Sauvola CW, et al. Elife. 2021 Oct 29;10:e72841. doi: 10.7554/eLife.72841. Elife. 2021. PMID: 34713802 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases