NF-kappa B-dependent and -independent pathways of HIV activation in a chronically infected T cell line - PubMed (original) (raw)
. 1994 Aug 1;202(2):684-94.
doi: 10.1006/viro.1994.1390.
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- PMID: 7913275
- DOI: 10.1006/viro.1994.1390
Free article
NF-kappa B-dependent and -independent pathways of HIV activation in a chronically infected T cell line
B A Antoni et al. Virology. 1994.
Free article
Abstract
J delta K cells were isolated as a chronically infected survivor cell line, following infection of Jurkat CD4+ T cells with dl-NF, a mutated strain of human immunodeficiency virus type 1 (HIV-1) containing a deletion of the long terminal repeat (LTR) NF-kappa B sites. J delta K cells exhibited very low levels of constitutive HIV production. HIV-1 expression was activated from J delta K cells by treatment with phorbol myristate acetate (PMA), sodium butyrate (NaB), or hexamethylene bisacetamide (HMBA), but not tumor necrosis factor alpha (TNF-alpha), confirming the role of NF-kappa B in mediating TNF-alpha induction of HIV transcription. The strong induction of HIV expression by NaB or HMBA in J delta K cells clearly demonstrates the existence of NF-kappa B-independent mechanisms of HIV activation in chronically infected cells. J delta K cells may provide a useful model for characterizing NF-kappa B-independent transcriptional activation of the HIV LTR.
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