Gypsy transposition correlates with the production of a retroviral envelope-like protein under the tissue-specific control of the Drosophila flamenco gene - PubMed (original) (raw)

Gypsy transposition correlates with the production of a retroviral envelope-like protein under the tissue-specific control of the Drosophila flamenco gene

A Pélisson et al. EMBO J. 1994.

Abstract

Gypsy displays striking similarities to vertebrate retroviruses, including the presence of a yet uncharacterized additional open reading frame (ORF3) and the recent evidence for infectivity. It is mobilized with high frequency in the germline of the progeny of females homozygous for the flamenco permissive mutation. We report the characterization of a gypsy subgenomic ORF3 RNA encoding typical retroviral envelope proteins. In females, env expression is strongly repressed by one copy of the non-permissive allele of flamenco. A less dramatic reduction in the accumulation of other transcripts and retrotranscripts is also observed. These effects correlate well with the inhibition of gypsy transposition in the progeny of these females, and are therefore likely to be responsible for this phenomenon. The effects of flamenco on gypsy expression are apparently restricted to the somatic follicle cells that surround the maternal germline. Moreover, permissive follicle cells display a typically polarized distribution of gypsy RNAs and envelope proteins, both being mainly accumulated at the apical pole, close to the oocyte. We propose a model suggesting that gypsy germinal transposition might occur only in individuals that have maternally inherited enveloped gypsy particles due to infection of the maternal germline by the soma.

PubMed Disclaimer

Cited by

Embryonic expression of endogenous retroviral RNAs in somatic tissues adjacent to the Oikopleura germline.
Henriet S, Sumic S, Doufoundou-Guilengui C, Jensen MF, Grandmougin C, Fal K, Thompson E, Volff JN, Chourrout D. Henriet S, et al. Nucleic Acids Res. 2015 Apr 20;43(7):3701-11. doi: 10.1093/nar/gkv169. Epub 2015 Mar 16. Nucleic Acids Res. 2015. PMID: 25779047 Free PMC article.
Identification of multiple transcription initiation, polyadenylation, and splice sites in the Drosophila melanogaster TART family of telomeric retrotransposons.
Maxwell PH, Belote JM, Levis RW. Maxwell PH, et al. Nucleic Acids Res. 2006;34(19):5498-507. doi: 10.1093/nar/gkl709. Epub 2006 Oct 4. Nucleic Acids Res. 2006. PMID: 17020919 Free PMC article.
A Transgenic Flock House Virus Replicon Reveals an RNAi Independent Antiviral Mechanism Acting in Drosophila Follicular Somatic Cells.
Martins N, Lemoine A, Santiago E, Paro S, Imler JL, Meignin C. Martins N, et al. G3 (Bethesda). 2019 Feb 7;9(2):403-412. doi: 10.1534/g3.118.200872. G3 (Bethesda). 2019. PMID: 30530643 Free PMC article.
Helena and BS: Two Travellers between the Genera Drosophila and Zaprionus.
Simão MC, Haudry A, Granzotto A, de Setta N, Carareto CMA. Simão MC, et al. Genome Biol Evol. 2018 Oct 1;10(10):2671-2685. doi: 10.1093/gbe/evy184. Genome Biol Evol. 2018. PMID: 30165545 Free PMC article.
A transposon expression burst accompanies the activation of Y-chromosome fertility genes during Drosophila spermatogenesis.
Lawlor MA, Cao W, Ellison CE. Lawlor MA, et al. Nat Commun. 2021 Nov 25;12(1):6854. doi: 10.1038/s41467-021-27136-4. Nat Commun. 2021. PMID: 34824217 Free PMC article.

References

1. Mol Cell Biol. 1994 Aug;14(8):5392-401 - PubMed
1. Mol Gen Genet. 1990 Sep;223(2):305-9 - PubMed
1. Genetics. 1982 Jul-Aug;101(3-4):461-76 - PubMed
1. Eur J Biochem. 1982 Apr;123(3):527-34 - PubMed
1. Mol Cell Biol. 1989 Jan;9(1):177-84 - PubMed

Gypsy transposition correlates with the production of a retroviral envelope-like protein under the tissue-specific control of the Drosophila flamenco gene - PubMed (original) (raw)