Integral proteins of the extracellular matrix fibrils of Myxococcus xanthus - PubMed (original) (raw)
Integral proteins of the extracellular matrix fibrils of Myxococcus xanthus
R M Behmlander et al. J Bacteriol. 1994 Oct.
Abstract
The extracellular matrix fibrils of Myxococcus xanthus are mediators of cell-cell cohesion and as such are required for the maintenance of the social lifestyle characteristic of these prokaryotes. The fibrils have also been implicated as factors involved in contact-mediated cell interactions and in signal exchange. The fibrils are extracellular carbohydrate structures with associated proteins. All of the major proteins associated with the fibrils react with monoclonal antibody 2105 and can be removed from the fibrils only by boiling with sodium dodecyl sulfate (SDS) and beta-mercaptoethanol. For consistency with their integral association with the fibrils, we have designated this class of proteins as integral fibrillar proteins class 1 (IFP-1). IFP-1 comprises five major proteins whose molecular sizes range from 66 to 14 kDa. All of the proteins in IFP-1 have been purified from isolated fibrils by electroelution after size separation on SDS-PAGE gels. Analysis of the purified proteins suggested that the forms with different molecular sizes result from the aggregation of a single small-molecular-size subunit. Fingerprint analysis and amino acid composition profiles confirmed the identity among the different members of IFP-1. The sequence of the 31 amino-terminal amino acids of the 31-kDa form of IFP-1 (IFP-1:31) was determined. There was no significant homology to other known protein sequences. During development there is a dramatic shift in the banding pattern of IFP-1 proteins without any apparent overall loss of total protein.
Similar articles
- A mutation that affects fibril protein, development, cohesion and gene expression in Myxococcus xanthus.
Smith DR, Dworkin M. Smith DR, et al. Microbiology (Reading). 1997 Dec;143 ( Pt 12):3683-3692. doi: 10.1099/00221287-143-12-3683. Microbiology (Reading). 1997. PMID: 9421894 - Biochemical and structural analyses of the extracellular matrix fibrils of Myxococcus xanthus.
Behmlander RM, Dworkin M. Behmlander RM, et al. J Bacteriol. 1994 Oct;176(20):6295-303. doi: 10.1128/jb.176.20.6295-6303.1994. J Bacteriol. 1994. PMID: 7929001 Free PMC article. - Isolated fibrils rescue cohesion and development in the Dsp mutant of Myxococcus xanthus.
Chang BY, Dworkin M. Chang BY, et al. J Bacteriol. 1994 Dec;176(23):7190-6. doi: 10.1128/jb.176.23.7190-7196.1994. J Bacteriol. 1994. PMID: 7961490 Free PMC article. - Fibrils as extracellular appendages of bacteria: their role in contact-mediated cell-cell interactions in Myxococcus xanthus.
Dworkin M. Dworkin M. Bioessays. 1999 Jul;21(7):590-5. doi: 10.1002/(SICI)1521-1878(199907)21:7<590::AID-BIES7>3.0.CO;2-E. Bioessays. 1999. PMID: 10472185 Review. - Extracellular biology of Myxococcus xanthus.
Konovalova A, Petters T, Søgaard-Andersen L. Konovalova A, et al. FEMS Microbiol Rev. 2010 Mar;34(2):89-106. doi: 10.1111/j.1574-6976.2009.00194.x. Epub 2009 Oct 20. FEMS Microbiol Rev. 2010. PMID: 19895646 Review.
Cited by
- Interplay of mesoscale physics and agent-like behaviors in the parallel evolution of aggregative multicellularity.
Arias Del Angel JA, Nanjundiah V, Benítez M, Newman SA. Arias Del Angel JA, et al. Evodevo. 2020 Oct 12;11:21. doi: 10.1186/s13227-020-00165-8. eCollection 2020. Evodevo. 2020. PMID: 33062243 Free PMC article. Review. - Myxobacteria: Moving, Killing, Feeding, and Surviving Together.
Muñoz-Dorado J, Marcos-Torres FJ, García-Bravo E, Moraleda-Muñoz A, Pérez J. Muñoz-Dorado J, et al. Front Microbiol. 2016 May 26;7:781. doi: 10.3389/fmicb.2016.00781. eCollection 2016. Front Microbiol. 2016. PMID: 27303375 Free PMC article. Review. - The orphan histidine protein kinase SgmT is a c-di-GMP receptor and regulates composition of the extracellular matrix together with the orphan DNA binding response regulator DigR in Myxococcus xanthus.
Petters T, Zhang X, Nesper J, Treuner-Lange A, Gomez-Santos N, Hoppert M, Jenal U, Søgaard-Andersen L. Petters T, et al. Mol Microbiol. 2012 Apr;84(1):147-65. doi: 10.1111/j.1365-2958.2012.08015.x. Epub 2012 Mar 6. Mol Microbiol. 2012. PMID: 22394314 Free PMC article. - The Myxococcus xanthus spore cuticula protein C is a fragment of FibA, an extracellular metalloprotease produced exclusively in aggregated cells.
Lee B, Mann P, Grover V, Treuner-Lange A, Kahnt J, Higgs PI. Lee B, et al. PLoS One. 2011;6(12):e28968. doi: 10.1371/journal.pone.0028968. Epub 2011 Dec 12. PLoS One. 2011. PMID: 22174937 Free PMC article. - Gliding motility revisited: how do the myxobacteria move without flagella?
Mauriello EM, Mignot T, Yang Z, Zusman DR. Mauriello EM, et al. Microbiol Mol Biol Rev. 2010 Jun;74(2):229-49. doi: 10.1128/MMBR.00043-09. Microbiol Mol Biol Rev. 2010. PMID: 20508248 Free PMC article. Review.
References
- J Bacteriol. 1977 Feb;129(2):770-7 - PubMed
- Can J Microbiol. 1976 Oct;22(10):1589-93 - PubMed
- Arch Microbiol. 1978 Jan 23;116(1):51-9 - PubMed
- J Bacteriol. 1978 Feb;133(2):763-8 - PubMed
- Infect Immun. 1978 Sep;21(3):978-88 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources