Interleukin-3 and bryostatin-1 mediate hyperphosphorylation of BCL2 alpha in association with suppression of apoptosis - PubMed (original) (raw)
Comparative Study
. 1994 Oct 28;269(43):26865-70.
Affiliations
- PMID: 7929424
Free article
Comparative Study
Interleukin-3 and bryostatin-1 mediate hyperphosphorylation of BCL2 alpha in association with suppression of apoptosis
W S May et al. J Biol Chem. 1994.
Free article
Abstract
Using murine myeloid factor-dependent FDC-P1/ER cells, we demonstrate that the hematopoietic growth factors interleukin-3 and erythropoietin and bryostatin-1, a macrocyclic lactone natural product and potent activator of protein kinase C (PKC), suppress apoptosis and induce the rapid serine phosphorylation of Bc12 alpha. Expression of recombinant wild type Bc12 alpha in NFS/N1.H-7 cells confirms that murine Bc12 alpha is phosphorylated following PKC activation. The PKC inhibitors H-7 and staurosporine, but not the protein kinase A inhibitor HA1004, block not only interleukin-3- and bryostatin-1-induced hyperphosphorylation of Bc12 alpha but also their anti-apoptotic effect on growth factor-dependent cells, suggesting a role for activated PKC in both processes. A potential direct role for a classic isoform of PKC is indicated by the Ca(2+)-dependent nature of phosphorylation of Bc12 alpha mediated by purified PKC in vitro. Comparative phosphopeptide maps confirm that Bc12 alpha phosphorylation occurs on identical serine site(s) whether phosphorylation occurs in cells following agonist treatment or directly by PKC in vitro. These findings strongly support a role for activated PKC in growth factor-induced Bc12 alpha phosphorylation as well as suppression of apoptosis.
Similar articles
- Protein kinase C-mediated serine phosphorylation directly activates Raf-1 in murine hematopoietic cells.
Carroll MP, May WS. Carroll MP, et al. J Biol Chem. 1994 Jan 14;269(2):1249-56. J Biol Chem. 1994. PMID: 8288587 - Reversible phosphorylation of Bcl2 following interleukin 3 or bryostatin 1 is mediated by direct interaction with protein phosphatase 2A.
Deng X, Ito T, Carr B, Mumby M, May WS Jr. Deng X, et al. J Biol Chem. 1998 Dec 18;273(51):34157-63. doi: 10.1074/jbc.273.51.34157. J Biol Chem. 1998. PMID: 9852076 - Regulation of Bcl2 phosphorylation and potential significance for leukemic cell chemoresistance.
Deng X, Kornblau SM, Ruvolo PP, May WS Jr. Deng X, et al. J Natl Cancer Inst Monogr. 2001;(28):30-7. doi: 10.1093/oxfordjournals.jncimonographs.a024254. J Natl Cancer Inst Monogr. 2001. PMID: 11158204 Review. - Intracellular protein phosphorylation in murine peritoneal macrophages in response to bacterial lipopolysaccharide (LPS): effects of kinase-inhibitors and LPS-induced tolerance.
Nakano M, Saito S, Nakano Y, Yamasu H, Matsuura M, Shinomiya H. Nakano M, et al. Immunobiology. 1993 Apr;187(3-5):272-82. doi: 10.1016/S0171-2985(11)80344-X. Immunobiology. 1993. PMID: 7687235 Review.
Cited by
- Multi-omics integration and interactomics reveals molecular networks and regulators of the beneficial effect of yoga and exercise.
Khokhar M, Tomo S, Gadwal A, Purohit P. Khokhar M, et al. Int J Yoga. 2022 Jan-Apr;15(1):25-39. doi: 10.4103/ijoy.ijoy_146_21. Epub 2022 Mar 21. Int J Yoga. 2022. PMID: 35444372 Free PMC article. - JAK3 mutations and mitochondrial apoptosis resistance in T-cell acute lymphoblastic leukemia.
Bodaar K, Yamagata N, Barthe A, Landrigan J, Chonghaile TN, Burns M, Stevenson KE, Devidas M, Loh ML, Hunger SP, Wood B, Silverman LB, Teachey DT, Meijerink JP, Letai A, Gutierrez A. Bodaar K, et al. Leukemia. 2022 Jun;36(6):1499-1507. doi: 10.1038/s41375-022-01558-5. Epub 2022 Apr 11. Leukemia. 2022. PMID: 35411095 Free PMC article. - Reactivating latent HIV with PKC agonists induces resistance to apoptosis and is associated with phosphorylation and activation of BCL2.
French AJ, Natesampillai S, Krogman A, Correia C, Peterson KL, Alto A, Chandrasekar AP, Misra A, Li Y, Kaufmann SH, Badley AD, Cummins NW. French AJ, et al. PLoS Pathog. 2020 Oct 19;16(10):e1008906. doi: 10.1371/journal.ppat.1008906. eCollection 2020 Oct. PLoS Pathog. 2020. PMID: 33075109 Free PMC article. - Systems analysis of phosphorylation-regulated Bcl-2 interactions establishes a model to reconcile the controversy over the significance of Bcl-2 phosphorylation.
Song T, Wang P, Yu X, Wang A, Chai G, Fan Y, Zhang Z. Song T, et al. Br J Pharmacol. 2019 Feb;176(3):491-504. doi: 10.1111/bph.14555. Epub 2018 Dec 26. Br J Pharmacol. 2019. PMID: 30500985 Free PMC article. - IL-4 enhances survival of in vitro-differentiated mouse basophils through transcription-independent signaling downstream of PI3K.
Reinhart R, Kaufmann T. Reinhart R, et al. Cell Death Dis. 2018 Jun 18;9(7):713. doi: 10.1038/s41419-018-0754-z. Cell Death Dis. 2018. PMID: 29915306 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Miscellaneous