The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase - PubMed (original) (raw)

The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase

M Shirayama et al. Mol Cell Biol. 1994 Nov.

Abstract

LTE1 belongs to the CDC25 family that encodes a guanine nucleotide exchange factor for GTP-binding proteins of the ras family. Previously we have shown that LTE1 is essential for termination of M phase at low temperatures. We have identified TEM1 as a gene that, when present on a multicopy plasmid, suppresses the cold-sensitive phenotype of lte1. Sequence analysis of TEM1 and GTP-binding analysis of the gene product revealed that TEM1 encodes a novel low-molecular-weight GTP-binding protein. The defect of TEM1 was lethal, and the tem1-defective cells were arrested at telophase with high H1-kinase activity under restrictive conditions, indicating that TEM1 is required to exit from M phase. The defect of TEM1 was suppressed by a high dose of CDC15, which encodes a protein kinase homologous to mitogen-activated protein kinase kinase kinases. The genetic interaction among LTE1, TEM1, and CDC15 indicates that they cooperatively play an essential role for termination of M phase.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Science. 1992 Sep 4;257(5075):1404-7 - PubMed
    1. Yeast. 1994 Apr;10(4):451-61 - PubMed
    1. Mol Cell Biol. 1992 Dec;12(12):5690-9 - PubMed
    1. Nucleic Acids Res. 1992 Nov 11;20(21):5617-23 - PubMed
    1. EMBO J. 1993 May;12(5):1969-78 - PubMed

MeSH terms

Substances

LinkOut - more resources