The SR protein B52/SRp55 is essential for Drosophila development - PubMed (original) (raw)

The SR protein B52/SRp55 is essential for Drosophila development

H Z Ring et al. Mol Cell Biol. 1994 Nov.

Abstract

B52, also called SRp55, is a 52-kDa member of the Drosophila SR protein family of general splicing factors. Escherichia coli-produced B52 is capable of both activating splicing and affecting the alternative splice site choice in human in vitro splicing reactions. Here we report the isolation of a B52 null mutant generated by remobilizing a P element residing near the B52 gene. The resulting deletion, B52(28), is confined to the B52 gene and its neighbor the Hrb87F gene. Second-instar larvae homozygous for the deletion are deficient in both B52 mRNA and protein. The B52 null mutant is lethal at the first- and second-instar larval stages. Germ line transformation of Drosophila flies with B52 genomic DNA rescues this lethality. Thus, B52 is an essential gene and has a critical role in Drosophila development. Larvae deficient in B52 are still capable of splicing the five endogenous pre-mRNAs tested here, including both constitutively and alternatively spliced genes. Therefore, B52 is not required for all splicing in vivo. This is the first in vivo deficiency analysis of a member of the SR protein family.

PubMed Disclaimer

Similar articles

• Genetic enhancement of RNA-processing defects by a dominant mutation in B52, the Drosophila gene for an SR protein splicing factor.
  Peng X, Mount SM. Peng X, et al. Mol Cell Biol. 1995 Nov;15(11):6273-82. doi: 10.1128/MCB.15.11.6273. Mol Cell Biol. 1995. PMID: 7565780 Free PMC article.
• Pre-mRNA splicing by the essential Drosophila protein B52: tissue and target specificity.
  Hoffman BE, Lis JT. Hoffman BE, et al. Mol Cell Biol. 2000 Jan;20(1):181-6. doi: 10.1128/MCB.20.1.181-186.2000. Mol Cell Biol. 2000. PMID: 10594020 Free PMC article.
• Specific SR protein-dependent splicing substrates identified through genomic SELEX.
  Kim S, Shi H, Lee DK, Lis JT. Kim S, et al. Nucleic Acids Res. 2003 Apr 1;31(7):1955-61. doi: 10.1093/nar/gkg286. Nucleic Acids Res. 2003. PMID: 12655012 Free PMC article.
• Functions of SR and Tra2 proteins in pre-mRNA splicing regulation.
  Tacke R, Manley JL. Tacke R, et al. Proc Soc Exp Biol Med. 1999 Feb;220(2):59-63. doi: 10.1046/j.1525-1373.1999.d01-10.x. Proc Soc Exp Biol Med. 1999. PMID: 9933499 Review. No abstract available.
• B52 promotes alternative splicing of Dscam in Chinese mitten crab, Eriocheir sinensis.
  Wan ZC, Li D, Li XJ, Zhu YT, Gao TH, Li WW, Wang Q. Wan ZC, et al. Fish Shellfish Immunol. 2019 Apr;87:460-469. doi: 10.1016/j.fsi.2019.01.027. Epub 2019 Jan 24. Fish Shellfish Immunol. 2019. PMID: 30685464 Review.

Cited by

• SRSF6 balances mitochondrial-driven innate immune outcomes through alternative splicing of BAX.
  Wagner AR, Weindel CG, West KO, Scott HM, Watson RO, Patrick KL. Wagner AR, et al. Elife. 2022 Nov 21;11:e82244. doi: 10.7554/eLife.82244. Elife. 2022. PMID: 36409059 Free PMC article.
• SRSF6 Regulates the Alternative Splicing of the Apoptotic Fas Gene by Targeting a Novel RNA Sequence.
  Choi N, Jang HN, Oh J, Ha J, Park H, Zheng X, Lee S, Shen H. Choi N, et al. Cancers (Basel). 2022 Apr 14;14(8):1990. doi: 10.3390/cancers14081990. Cancers (Basel). 2022. PMID: 35454897 Free PMC article.
• Targeting Splicing Factor SRSF6 for Cancer Therapy.
  She W, Shao J, Jia R. She W, et al. Front Cell Dev Biol. 2021 Nov 30;9:780023. doi: 10.3389/fcell.2021.780023. eCollection 2021. Front Cell Dev Biol. 2021. PMID: 34917618 Free PMC article. Review.
• The SR protein B52/SRp55 regulates splicing of the period thermosensitive intron and mid-day siesta in Drosophila.
  Zhang Z, Cao W, Edery I. Zhang Z, et al. Sci Rep. 2018 Jan 30;8(1):1872. doi: 10.1038/s41598-017-18167-3. Sci Rep. 2018. PMID: 29382842 Free PMC article.
• Number of inadvertent RNA targets for morpholino knockdown in Danio rerio is largely underestimated: evidence from the study of Ser/Arg-rich splicing factors.
  Joris M, Schloesser M, Baurain D, Hanikenne M, Muller M, Motte P. Joris M, et al. Nucleic Acids Res. 2017 Sep 19;45(16):9547-9557. doi: 10.1093/nar/gkx638. Nucleic Acids Res. 2017. PMID: 28934490 Free PMC article.

References

1. 1. Cell. 1989 Apr 7;57(1):89-101 - PubMed
2. 1. Mol Cell Biol. 1994 Aug;14(8):5360-70 - PubMed
3. 1. Genes Dev. 1989 Sep;3(9):1273-87 - PubMed
4. 1. Mol Cell Biol. 1990 Jun;10(6):2492-502 - PubMed
5. 1. J Mol Biol. 1977 Feb 5;109(4):559-87 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Taylor & Francis

• Other Literature Sources

  • The Lens - Patent Citations

• Molecular Biology Databases

  • FlyBase
  • GlyGen glycoinformatics resource

• Research Materials

  • NCI CPTC Antibody Characterization Program