Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation - PubMed (original) (raw)
Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation
I Ingelbrecht et al. Proc Natl Acad Sci U S A. 1994.
Abstract
Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical motifs CG and CXG.
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