Microtubule severing by elongation factor 1 alpha - PubMed (original) (raw)
. 1994 Oct 14;266(5183):282-5.
doi: 10.1126/science.7939665.
Affiliations
- PMID: 7939665
- DOI: 10.1126/science.7939665
Microtubule severing by elongation factor 1 alpha
N Shiina et al. Science. 1994.
Abstract
An activity that severs stable microtubules is thought to be involved in microtubule reorganization during the cell cycle. Here, a 48-kilodalton microtubule-severing protein was purified from Xenopus eggs and identified as translational elongation factor 1 alpha (EF-1 alpha). Bacterially expressed human EF-1 alpha also displayed microtubule-severing activity in vitro and, when microinjected into fibroblasts, induced rapid and transient fragmentation of cytoplasmic microtubule arrays. Thus, EF-1 alpha, an essential component of the eukaryotic translational apparatus, appears to have a second role as a regulator of cytoskeletal rearrangements.
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