Exposure to hydrogen peroxide induces cell death via apoptosis in cultured rat cortical neurons - PubMed (original) (raw)

Exposure to hydrogen peroxide induces cell death via apoptosis in cultured rat cortical neurons

E R Whittemore et al. Neuroreport. 1994.

Abstract

To investigate whether direct oxidative damage may induce neuronal death via an apoptotic cell suicide pathway, we exposed cultured cortical neurons to the prooxidant hydrogen peroxide. Our experiments demonstrate that brief exposures to low concentrations of hydrogen peroxide can induce neuronal cell death via apoptosis as demonstrated by toxicity assays, specific DNA staining, and the appearance of DNA laddering on agarose gels. These results suggest that direct oxidative injury may serve as a general trigger for apoptosis in the CNS, and that other apoptotic stimuli, such as exposure to amyloid-beta peptides, may also involve oxidative injury. Peroxide-induced apoptosis may serve as a valuable model for further studies of apoptosis in neurons.

PubMed Disclaimer

Similar articles

• Exposure to hydrogen peroxide induces cell death via apoptosis in primary cultured mouse hepatocytes.
  Kanno S, Ishikawa M, Takayanagi M, Takayanagi Y, Sasaki K. Kanno S, et al. Biol Pharm Bull. 1999 Dec;22(12):1296-300. doi: 10.1248/bpb.22.1296. Biol Pharm Bull. 1999. PMID: 10746159
• The nonfibrillar amyloid beta-peptide induces apoptotic neuronal cell death: involvement of its C-terminal fusogenic domain.
  Pillot T, Drouet B, Queillé S, Labeur C, Vandekerchkhove J, Rosseneu M, Pinçon-Raymond M, Chambaz J. Pillot T, et al. J Neurochem. 1999 Oct;73(4):1626-34. doi: 10.1046/j.1471-4159.1999.0731626.x. J Neurochem. 1999. PMID: 10501209
• Generation of hydrogen peroxide during brief oxygen-glucose deprivation induces preconditioning neuronal protection in primary cultured neurons.
  Furuichi T, Liu W, Shi H, Miyake M, Liu KJ. Furuichi T, et al. J Neurosci Res. 2005 Mar 15;79(6):816-24. doi: 10.1002/jnr.20402. J Neurosci Res. 2005. PMID: 15668910
• Glutathione peroxidase 4 protects cortical neurons from oxidative injury and amyloid toxicity.
  Ran Q, Gu M, Van Remmen H, Strong R, Roberts JL, Richardson A. Ran Q, et al. J Neurosci Res. 2006 Jul;84(1):202-8. doi: 10.1002/jnr.20868. J Neurosci Res. 2006. PMID: 16673405

Cited by

• Cellular Pre-Adaptation to the High O2 Concentration Used in Standard Cell Culture Confers Resistance to Subsequent H2O2-Induced Cell Death.
  Jordan JB, Smallwood MJ, Smerdon GR, Winyard PG. Jordan JB, et al. Antioxidants (Basel). 2024 Feb 22;13(3):269. doi: 10.3390/antiox13030269. Antioxidants (Basel). 2024. PMID: 38539802 Free PMC article.
• Study of Chitosan-Stabilized Ti3C2T_x_ MXene for Ultrasensitive and Interference-Free Detection of Gaseous H2O2.
  Isailović J, Oberlintner A, Novak U, Finšgar M, Oliveira FM, Paštika J, Sofer Z, Tasić N, Gusmão R, Hočevar SB. Isailović J, et al. ACS Appl Mater Interfaces. 2023 Jul 5;15(26):31643-31651. doi: 10.1021/acsami.3c05314. Epub 2023 Jun 23. ACS Appl Mater Interfaces. 2023. PMID: 37350261 Free PMC article.
• Water-Soluble Total Flavonoids Isolated from Isodon lophanthoides var_. gerardianus_ (Benth.) H. Hara Promote Hepatocellular Carcinoma Sensitivity to 5-Fluorouracil.
  Feng CP, Ding HX, Liu YX, Di QF, Liu Y, Liang J, Liu GX. Feng CP, et al. Evid Based Complement Alternat Med. 2021 Jul 17;2021:6623212. doi: 10.1155/2021/6623212. eCollection 2021. Evid Based Complement Alternat Med. 2021. PMID: 34335833 Free PMC article.
• Neuroprotective Effect of Cannabidiol Against Hydrogen Peroxide in Hippocampal Neuron Culture.
  Kim J, Choi JY, Seo J, Choi IS. Kim J, et al. Cannabis Cannabinoid Res. 2021 Feb 12;6(1):40-47. doi: 10.1089/can.2019.0102. eCollection 2021. Cannabis Cannabinoid Res. 2021. PMID: 33614951 Free PMC article.
• TRPM2 channel-mediated cell death: An important mechanism linking oxidative stress-inducing pathological factors to associated pathological conditions.
  Malko P, Jiang LH. Malko P, et al. Redox Biol. 2020 Oct;37:101755. doi: 10.1016/j.redox.2020.101755. Epub 2020 Oct 16. Redox Biol. 2020. PMID: 33130440 Free PMC article. Review.

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Ovid Technologies, Inc.