A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro - PubMed (original) (raw)

Comparative Study

. 1994 Nov 4;79(3):449-58.

doi: 10.1016/0092-8674(94)90254-2.

Affiliations

• PMID: 7954811
• DOI: 10.1016/0092-8674(94)90254-2

Comparative Study

A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro

T Hirano et al. Cell. 1994.

Abstract

We report here a chromosomal protein that plays an essential role in mitotic chromosome condensation in Xenopus egg extracts. Two polypeptides, designated XCAP-C and XCAP-E, were found to associate with each other in the extracts, presumably forming a heterodimer. During chromosome assembly in mitotic extracts, XCAP-C/E was recruited to the chromatin and formed a discrete internal structure within assembled chromosomes. Antibody blocking experiments showed that XCAP-C function is required for both assembly and structural maintenance of mitotic chromosomes in vitro. Deduced amino acid sequences revealed that the two polypeptides share common structural motifs, consisting of an N-terminal NTP-binding domain, two central coiled-coil regions, and a C-terminal conserved domain. These motifs are highly conserved in a protein family, members of which have been identified recently in both prokaryotes and eukaryotes.

PubMed Disclaimer

Similar articles

• A human condensin complex containing hCAP-C-hCAP-E and CNAP1, a homolog of Xenopus XCAP-D2, colocalizes with phosphorylated histone H3 during the early stage of mitotic chromosome condensation.
  Schmiesing JA, Gregson HC, Zhou S, Yokomori K. Schmiesing JA, et al. Mol Cell Biol. 2000 Sep;20(18):6996-7006. doi: 10.1128/MCB.20.18.6996-7006.2000. Mol Cell Biol. 2000. PMID: 10958694 Free PMC article.
• Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein.
  Hirano T, Kobayashi R, Hirano M. Hirano T, et al. Cell. 1997 May 16;89(4):511-21. doi: 10.1016/s0092-8674(00)80233-0. Cell. 1997. PMID: 9160743
• Identification of two distinct human SMC protein complexes involved in mitotic chromosome dynamics.
  Schmiesing JA, Ball AR Jr, Gregson HC, Alderton JM, Zhou S, Yokomori K. Schmiesing JA, et al. Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):12906-11. doi: 10.1073/pnas.95.22.12906. Proc Natl Acad Sci U S A. 1998. PMID: 9789013 Free PMC article.
• Shaping the metaphase chromosome: coordination of cohesion and condensation.
  Losada A, Hirano T. Losada A, et al. Bioessays. 2001 Oct;23(10):924-35. doi: 10.1002/bies.1133. Bioessays. 2001. PMID: 11598959 Review.
• The SMC proteins and the coming of age of the chromosome scaffold hypothesis.
  Saitoh N, Goldberg I, Earnshaw WC. Saitoh N, et al. Bioessays. 1995 Sep;17(9):759-66. doi: 10.1002/bies.950170905. Bioessays. 1995. PMID: 8763828 Review.

Cited by

• Structural Maintenance of Chromosomes Complexes.
  Jeppsson K. Jeppsson K. Methods Mol Biol. 2025;2856:11-22. doi: 10.1007/978-1-0716-4136-1_2. Methods Mol Biol. 2025. PMID: 39283444 Review.
• Quantitative imaging of loop extruders rebuilding interphase genome architecture after mitosis.
  Brunner A, Morero NR, Zhang W, Hossain MJ, Lampe M, Pflaumer H, Halavatyi A, Peters JM, Beckwith KS, Ellenberg J. Brunner A, et al. bioRxiv [Preprint]. 2024 May 30:2024.05.29.596439. doi: 10.1101/2024.05.29.596439. bioRxiv. 2024. PMID: 38854067 Free PMC article. Preprint.
• Rules of engagement for condensins and cohesins guide mitotic chromosome formation.
  Samejima K, Gibcus JH, Abraham S, Cisneros-Soberanis F, Samejima I, Beckett AJ, Pučeková N, Abad MA, Medina-Pritchard B, Paulson JR, Xie L, Jeyaprakash AA, Prior IA, Mirny LA, Dekker J, Goloborodko A, Earnshaw WC. Samejima K, et al. bioRxiv [Preprint]. 2024 Apr 30:2024.04.18.590027. doi: 10.1101/2024.04.18.590027. bioRxiv. 2024. PMID: 38659940 Free PMC article. Preprint.
• Recombinant cyclin B-Cdk1-Suc1 capable of multi-site mitotic phosphorylation in vitro.
  Shintomi K, Masahara-Negishi Y, Shima M, Tane S, Hirano T. Shintomi K, et al. PLoS One. 2024 Mar 25;19(3):e0299003. doi: 10.1371/journal.pone.0299003. eCollection 2024. PLoS One. 2024. PMID: 38527022 Free PMC article.
• Single-Molecule Approaches to Study DNA Condensation.
  Golfier S, Quail T, Brugués J. Golfier S, et al. Methods Mol Biol. 2024;2740:1-19. doi: 10.1007/978-1-0716-3557-5_1. Methods Mol Biol. 2024. PMID: 38393466

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations