Synaptotagmin I: a major Ca2+ sensor for transmitter release at a central synapse - PubMed (original) (raw)
Comparative Study
. 1994 Nov 18;79(4):717-27.
doi: 10.1016/0092-8674(94)90556-8.
Affiliations
- PMID: 7954835
- DOI: 10.1016/0092-8674(94)90556-8
Comparative Study
Synaptotagmin I: a major Ca2+ sensor for transmitter release at a central synapse
M Geppert et al. Cell. 1994.
Abstract
Mice carrying a mutation in the synaptotagmin I gene were generated by homologous recombination. Mutant mice are phenotypically normal as heterozygotes, but die within 48 hr after birth as homozygotes. Studies of hippocampal neurons cultured from homozygous mutant mice reveal that synaptic transmission is severely impaired. The synchronous, fast component of Ca(2+)-dependent neurotransmitter release is decreased, whereas asynchronous release processes, including spontaneous synaptic activity (miniature excitatory postsynaptic current frequency) and release triggered by hypertonic solution or alpha-latrotoxin, are unaffected. Our findings demonstrate that synaptotagmin I function is required for Ca2+ triggering of synchronous neurotransmitter release, but is not essential for asynchronous or Ca(2+)-independent release. We propose that synaptotagmin I is the major low affinity Ca2+ sensor mediating Ca2+ regulation of synchronous neurotransmitter release in hippocampal neurons.
Similar articles
- Dual roles of the C2B domain of synaptotagmin I in synchronizing Ca2+-dependent neurotransmitter release.
Nishiki T, Augustine GJ. Nishiki T, et al. J Neurosci. 2004 Sep 29;24(39):8542-50. doi: 10.1523/JNEUROSCI.2545-04.2004. J Neurosci. 2004. PMID: 15456828 Free PMC article. - Synaptotagmin I functions as a calcium regulator of release probability.
Fernández-Chacón R, Königstorfer A, Gerber SH, García J, Matos MF, Stevens CF, Brose N, Rizo J, Rosenmund C, Südhof TC. Fernández-Chacón R, et al. Nature. 2001 Mar 1;410(6824):41-9. doi: 10.1038/35065004. Nature. 2001. PMID: 11242035 - Synaptotagmin I synchronizes transmitter release in mouse hippocampal neurons.
Nishiki T, Augustine GJ. Nishiki T, et al. J Neurosci. 2004 Jul 7;24(27):6127-32. doi: 10.1523/JNEUROSCI.1563-04.2004. J Neurosci. 2004. PMID: 15240804 Free PMC article. - Synaptotagmin I, a Ca2+ sensor for neurotransmitter release.
Koh TW, Bellen HJ. Koh TW, et al. Trends Neurosci. 2003 Aug;26(8):413-22. doi: 10.1016/S0166-2236(03)00195-4. Trends Neurosci. 2003. PMID: 12900172 Review. - Synaptotagmin controls and modulates synaptic-vesicle fusion in a Ca(2+)-dependent manner.
Littleton JT, Bellen HJ. Littleton JT, et al. Trends Neurosci. 1995 Apr;18(4):177-83. doi: 10.1016/0166-2236(95)93898-8. Trends Neurosci. 1995. PMID: 7778189 Review.
Cited by
- Neuropathological mRNA Expression Changes after Single Mild Traumatic Brain Injury in Pigs.
Grovola MR, Cullen DK. Grovola MR, et al. Biomedicines. 2024 Sep 4;12(9):2019. doi: 10.3390/biomedicines12092019. Biomedicines. 2024. PMID: 39335533 Free PMC article. - Synaptotagmin-9 in mouse retina.
Mesnard CS, Hays CL, Townsend LE, Barta CL, Gurumurthy CB, Thoreson WB. Mesnard CS, et al. Vis Neurosci. 2024 Sep 18;41:E003. doi: 10.1017/S0952523824000026. Vis Neurosci. 2024. PMID: 39291699 Free PMC article. - A guide to selecting high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot, immunoprecipitation, immunofluorescence and flow cytometry.
Biddle MS, Alende C, Fotouhi M, Jones C, Ayoubi R, Southern K, Laflamme C, Virk H; NeuroSGC/YCharOS/EDDU collaborative group; ABIF consortium. Biddle MS, et al. F1000Res. 2024 Jul 19;13:817. doi: 10.12688/f1000research.154034.1. eCollection 2024. F1000Res. 2024. PMID: 39169954 Free PMC article. - Synaptotagmins 3 and 7 mediate the majority of asynchronous release from synapses in the cerebellum and hippocampus.
Weingarten DJ, Shrestha A, Orlin DJ, Le Moing CL, Borchardt LA, Jackman SL. Weingarten DJ, et al. Cell Rep. 2024 Aug 27;43(8):114595. doi: 10.1016/j.celrep.2024.114595. Epub 2024 Aug 6. Cell Rep. 2024. PMID: 39116209 Free PMC article. - Region-Related Differences in Short-Term Synaptic Plasticity and Synaptotagmin-7 in the Male and Female Hippocampus of a Rat Model of Fragile X Syndrome.
Tsotsokou G, Miliou A, Trompoukis G, Leontiadis LJ, Papatheodoropoulos C. Tsotsokou G, et al. Int J Mol Sci. 2024 Jun 26;25(13):6975. doi: 10.3390/ijms25136975. Int J Mol Sci. 2024. PMID: 39000085 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous