Members of the HNF-3/forkhead family of transcription factors exhibit distinct cellular expression patterns in lung and regulate the surfactant protein B promoter - PubMed (original) (raw)

Comparative Study

. 1994 Nov;166(1):195-209.

doi: 10.1006/dbio.1994.1307.

Affiliations

• PMID: 7958446
• DOI: 10.1006/dbio.1994.1307

Comparative Study

Members of the HNF-3/forkhead family of transcription factors exhibit distinct cellular expression patterns in lung and regulate the surfactant protein B promoter

D E Clevidence et al. Dev Biol. 1994 Nov.

Abstract

The hepatocyte nuclear factor-3 (HNF-3)/forkhead (fkh) proteins consist of an extensive family of tissue-specific and developmental gene regulators which share homology within the winged helix DNA binding motif. We report on the isolation of a new family member, HNF-3/forkhead homolog 8 (HFH-8), from lung cDNA libraries and the derivation of the complete amino acid sequences for the HFH-8 protein as well as previously identified HFH-1 and HFH-4 proteins. The HFH proteins contain several sequence motifs found in activation domains of other transcription factors and HNF-3/fkh family members. In situ hybridization with the HNF-3, HFH-4, and HFH-8 probes in adult lung demonstrate that the HNF-3/fkh cellular expression patterns are regionally specified. Whereas HNF-3 alpha and HNF-3 beta are normally coexpressed in the hepatocyte, their expression patterns in the lung are different. The HNF-3 alpha and HFH-4 genes are coexpressed in the bronchiolar epithelium (clara cells), whereas the HNF-3 beta probe exhibits prominent hybridization with the smooth muscle surrounding arterioles and bronchioles. In contrast, HFH-8 probes labeled the type II pneumocyte cells lining the respiratory surfaces of terminal bronchioles and alveolar sac. We have identified an HNF-3 consensus DNA binding sequence in the proximal surfactant protein B (SPB) promoter region (SPB-f2, -78 to -88). SPB gene transcription is restricted to bronchiolar and alveolar epithelium which colocalizes with the expression pattern of the HNF-3 alpha and HFH-8 genes, respectively. We show that the SPB-f2 sequence is recognized by both HNF-3 alpha and HFH-8 proteins and that these cDNA expression vectors activate the SPB promoter in cotransfection assays through the HNF-3 consensus sequence. Our results suggest that SPB promoter activity is regulated by HNF-3 alpha and HFH-8 proteins in a cell type-specific manner.

PubMed Disclaimer

Similar articles

• The cut-homeodomain transcriptional activator HNF-6 is coexpressed with its target gene HNF-3 beta in the developing murine liver and pancreas.
  Rausa F, Samadani U, Ye H, Lim L, Fletcher CF, Jenkins NA, Copeland NG, Costa RH. Rausa F, et al. Dev Biol. 1997 Dec 15;192(2):228-46. doi: 10.1006/dbio.1997.8744. Dev Biol. 1997. PMID: 9441664
• Mechanisms of gene expression and cell fate determination in the developing pulmonary epithelium.
  Hackett BP, Bingle CD, Gitlin JD. Hackett BP, et al. Annu Rev Physiol. 1996;58:51-71. doi: 10.1146/annurev.ph.58.030196.000411. Annu Rev Physiol. 1996. PMID: 8815806 Review.
• Regulation of the Clara cell secretory protein/uteroglobin promoter in lung.
  Nord M, Cassel TN, Braun H, Suske G. Nord M, et al. Ann N Y Acad Sci. 2000;923:154-65. doi: 10.1111/j.1749-6632.2000.tb05527.x. Ann N Y Acad Sci. 2000. PMID: 11193754 Review.

Cited by

• The whn transcription factor encoded by the nude locus contains an evolutionarily conserved and functionally indispensable activation domain.
  Schüddekopf K, Schorpp M, Boehm T. Schüddekopf K, et al. Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9661-4. doi: 10.1073/pnas.93.18.9661. Proc Natl Acad Sci U S A. 1996. PMID: 8790387 Free PMC article.
• Hepatocyte nuclear factor 3 activates transcription of thyroid transcription factor 1 in respiratory epithelial cells.
  Ikeda K, Shaw-White JR, Wert SE, Whitsett JA. Ikeda K, et al. Mol Cell Biol. 1996 Jul;16(7):3626-36. doi: 10.1128/MCB.16.7.3626. Mol Cell Biol. 1996. PMID: 8668179 Free PMC article.
• GemC1 controls multiciliogenesis in the airway epithelium.
  Arbi M, Pefani DE, Kyrousi C, Lalioti ME, Kalogeropoulou A, Papanastasiou AD, Taraviras S, Lygerou Z. Arbi M, et al. EMBO Rep. 2016 Mar;17(3):400-13. doi: 10.15252/embr.201540882. Epub 2016 Feb 4. EMBO Rep. 2016. PMID: 26882546 Free PMC article.
• The long terminal repeat of Jaagsiekte sheep retrovirus is preferentially active in differentiated epithelial cells of the lungs.
  Palmarini M, Datta S, Omid R, Murgia C, Fan H. Palmarini M, et al. J Virol. 2000 Jul;74(13):5776-87. doi: 10.1128/jvi.74.13.5776-5787.2000. J Virol. 2000. PMID: 10846056 Free PMC article.
• Hepatocyte nuclear factor 6, a transcription factor that contains a novel type of homeodomain and a single cut domain.
  Lemaigre FP, Durviaux SM, Truong O, Lannoy VJ, Hsuan JJ, Rousseau GG. Lemaigre FP, et al. Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9460-4. doi: 10.1073/pnas.93.18.9460. Proc Natl Acad Sci U S A. 1996. PMID: 8790352 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Medical

  • MedlinePlus Health Information

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • GlyGen glycoinformatics resource
  • Mouse Genome Informatics (MGI)

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal