Differential regulation of E2F transactivation by cyclin/cdk2 complexes - PubMed (original) (raw)
. 1994 Aug 1;8(15):1772-86.
doi: 10.1101/gad.8.15.1772.
Affiliations
- PMID: 7958856
- DOI: 10.1101/gad.8.15.1772
Free article
Differential regulation of E2F transactivation by cyclin/cdk2 complexes
B D Dynlacht et al. Genes Dev. 1994.
Free article
Abstract
The mammalian transcription factor E2F plays a critical role in the expression of genes required for cellular proliferation. To understand how E2F is regulated, we have developed a reconstituted in vitro transcription assay. Using this E2F-responsive assay, we can demonstrate that E2F-mediated transcription can be directly repressed by the tumor suppressor protein pRB. This inhibition is abolished by phosphorylation of pRB with either cyclin A/cdk2 or cyclin E/cdk2. However, these cyclin/kinase complexes exhibit differences in the ability to phosphorylate E2F. Only cyclin A/cdk2 can phosphorylate E2F effectively, and this phosphorylation abolishes its ability to bind DNA and mediate trans-activation. Thus, this in vitro transcriptional assay allows activation and inactivation of E2F transcription, and our findings demonstrate how transcriptional regulation of E2F can be linked to cell cycle-dependent activation of kinases.
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