Specific incorporation of cyclophilin A into HIV-1 virions - PubMed (original) (raw)
. 1994 Nov 24;372(6504):359-62.
doi: 10.1038/372359a0.
Affiliations
- PMID: 7969494
- DOI: 10.1038/372359a0
Specific incorporation of cyclophilin A into HIV-1 virions
E K Franke et al. Nature. 1994.
Abstract
Little is known about host factors necessary for retroviral virion assembly or uncoating. We have previously shown that the principal structural protein of the human immunodeficiency virus HIV-1, the Gag polyprotein, binds the cyclophilin peptidyl-prolyl isomerases; cyclophilins catalyse a rate-limiting step in protein folding and protect cells from heat shock. Here we demonstrate that cyclophilin A is specifically incorporated into HIV-1 virions but not into virions of other primate immunodeficiency viruses. A proline-rich region conserved in all HIV-1 Gag polyproteins is required for cyclophilin A binding and incorporation. Disruption of a single proline blocks the Gag-cyclophilin interaction in vitro, prevents cyclophilin A incorporation into virions, and inhibits HIV-1 replication. Our results indicate that the interaction of Gag with cyclophilin A is necessary for the formation of infectious HIV-1 virions.
Comment in
- Human immunodeficiency virus. Chaperoning a pathogen.
Cullen BR, Heitman J. Cullen BR, et al. Nature. 1994 Nov 24;372(6504):319-20. doi: 10.1038/372319a0. Nature. 1994. PMID: 7526224 No abstract available.
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