Human immunodeficiency virus type 1 MA deletion mutants expressed in baculovirus-infected cells: cis and trans effects on the Gag precursor assembly pathway - PubMed (original) (raw)
Human immunodeficiency virus type 1 MA deletion mutants expressed in baculovirus-infected cells: cis and trans effects on the Gag precursor assembly pathway
N Chazal et al. J Virol. 1995 Jan.
Abstract
The role of the matrix protein (MA) of human immunodeficiency virus type 1 in intracellular transport, assembly, and extracellular release of Gag polyprotein precursor (Pr55gag) was investigated by deletion mutagenesis of the MA domain of recombinant Gag precursor expressed in baculovirus-infected cells. In addition, three carboxy-terminally truncated forms of the Gag precursor, representing mainly the MA, were constructed. One corresponded to an MA with a deletion of its last 12 residues (amb120), while the others corresponded to the entire MA with an additional sequence from the N-terminal portion of the CA (amb143 and och180). Deletions within the MA central region (residues 41 to 78) appeared to be detrimental to Gag particle assembly and budding from the plasma membrane. A slightly narrower domain, between amino acids 41 and 68, was found to be critical for soluble Gag secretion. Mutations which totally or partially deleted one or the other of the two polybasic signals altered the transport of N-myristylated Gag precursor to the plasma membrane. In coexpression with wild-type Gag precursor, a discrete trans-dominant negative effect on wild-type Gag particle assembly and release was observed with deletion mutants located in the central MA region (residues 41 to 78). A more significant negative effect was obtained with the two recombinant proteins of amb120 and och180, which redirected the Gag particle assembly pathway from the plasma membrane compartment to intracellular vesicles (amb120) and to the nuclear compartment (och180).
Similar articles
- Phenotypic characterization of insertion mutants of the human immunodeficiency virus type 1 Gag precursor expressed in recombinant baculovirus-infected cells.
Chazal N, Carrière C, Gay B, Boulanger P. Chazal N, et al. J Virol. 1994 Jan;68(1):111-22. doi: 10.1128/JVI.68.1.111-122.1994. J Virol. 1994. PMID: 8254720 Free PMC article. - Identification of human immunodeficiency virus type 1 Gag protein domains essential to membrane binding and particle assembly.
Spearman P, Wang JJ, Vander Heyden N, Ratner L. Spearman P, et al. J Virol. 1994 May;68(5):3232-42. doi: 10.1128/JVI.68.5.3232-3242.1994. J Virol. 1994. PMID: 8151785 Free PMC article. - Membrane binding of human immunodeficiency virus type 1 matrix protein in vivo supports a conformational myristyl switch mechanism.
Spearman P, Horton R, Ratner L, Kuli-Zade I. Spearman P, et al. J Virol. 1997 Sep;71(9):6582-92. doi: 10.1128/JVI.71.9.6582-6592.1997. J Virol. 1997. PMID: 9261380 Free PMC article. - Post-Translational Modifications of Retroviral HIV-1 Gag Precursors: An Overview of Their Biological Role.
Bussienne C, Marquet R, Paillart JC, Bernacchi S. Bussienne C, et al. Int J Mol Sci. 2021 Mar 11;22(6):2871. doi: 10.3390/ijms22062871. Int J Mol Sci. 2021. PMID: 33799890 Free PMC article. Review. - Roles played by acidic lipids in HIV-1 Gag membrane binding.
Olety B, Ono A. Olety B, et al. Virus Res. 2014 Nov 26;193:108-15. doi: 10.1016/j.virusres.2014.06.015. Epub 2014 Jul 3. Virus Res. 2014. PMID: 24998886 Free PMC article. Review.
Cited by
- Biophysical characterization and crystal structure of the Feline Immunodeficiency Virus p15 matrix protein.
Serrière J, Robert X, Perez M, Gouet P, Guillon C. Serrière J, et al. Retrovirology. 2013 Jun 24;10:64. doi: 10.1186/1742-4690-10-64. Retrovirology. 2013. PMID: 23800358 Free PMC article. - A novel platform for virus-like particle-display of flaviviral envelope domain III: induction of Dengue and West Nile virus neutralizing antibodies.
Chua AJ, Vituret C, Tan ML, Gonzalez G, Boulanger P, Ng ML, Hong SS. Chua AJ, et al. Virol J. 2013 Apr 24;10:129. doi: 10.1186/1743-422X-10-129. Virol J. 2013. PMID: 23617954 Free PMC article. - Characterization of a novel type of HIV-1 particle assembly inhibitor using a quantitative luciferase-Vpr packaging-based assay.
Gonzalez G, DaFonseca S, Errazuriz E, Coric P, Souquet F, Turcaud S, Boulanger P, Bouaziz S, Hong SS. Gonzalez G, et al. PLoS One. 2011;6(11):e27234. doi: 10.1371/journal.pone.0027234. Epub 2011 Nov 3. PLoS One. 2011. PMID: 22073298 Free PMC article. - Baculovirus display of single chain antibody (scFv) using a novel signal peptide.
Kitidee K, Nangola S, Gonzalez G, Boulanger P, Tayapiwatana C, Hong SS. Kitidee K, et al. BMC Biotechnol. 2010 Nov 19;10:80. doi: 10.1186/1472-6750-10-80. BMC Biotechnol. 2010. PMID: 21092083 Free PMC article. - Human Polycomb group EED protein negatively affects HIV-1 assembly and release.
Rakotobe D, Tardy JC, André P, Hong SS, Darlix JL, Boulanger P. Rakotobe D, et al. Retrovirology. 2007 Jun 4;4:37. doi: 10.1186/1742-4690-4-37. Retrovirology. 2007. PMID: 17547741 Free PMC article.
References
- Nature. 1970 Aug 15;227(5259):680-5 - PubMed
- J Virol. 1994 Aug;68(8):5311-20 - PubMed
- J Virol. 1984 Mar;49(3):909-17 - PubMed
- Cell. 1985 Jan;40(1):9-17 - PubMed
- Proc Natl Acad Sci U S A. 1986 Oct;83(19):7246-50 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources