Transfer RNA binding to 80S ribosomes from yeast: evidence for three sites - PubMed (original) (raw)
Affiliations
- PMID: 7987260
Transfer RNA binding to 80S ribosomes from yeast: evidence for three sites
F Triana et al. Biochem Mol Biol Int. 1994 Aug.
Abstract
The number of tRNA binding sites in 80S ribosomes from Saccharomyces cerevisiae was assessed by means of tRNA saturation and translocation experiments. In the absence of cognate mRNA yeast ribosomes could bind 0.6 [32P]tRNA(Phe) per 80S while poly(U) programmed ribosomes accepted up to 1.7 tRNA(Phe) molecules per 80S or 0.5 molecules of Ac[14C]Phe-tRNA(Phe) per 80S. Compared with the known features of E. coli ribosomes these binding values indicated both the presence of three tRNA binding sites and the validity of the exclusion principle for peptidyl-tRNA binding to yeast ribosomes. Upon EF-2 dependent translocation of a complex containing deacyl-tRNA in the P-site and AcPHe-tRNA in the A-site, the deacylated tRNA does not leave the ribosome quantitatively. This observation suggests the presence of an E site in 80S ribosomes which is functionally equivalent to the one previously characterized in prokaryotic systems.
Similar articles
- [Characteristics of nonenzymatic binding of oligoribonucleotides-- analogs of mRNA and Phe-tRNA Phe with 80S ribosomes from human placenta].
Graĭfer DM, Zenkova MA, Karpova GG, Malygin AA, Matasova NB. Graĭfer DM, et al. Mol Biol (Mosk). 1990 Jul-Aug;24(4):1076-83. Mol Biol (Mosk). 1990. PMID: 2250674 Russian. - Analysis of the puromycin reaction. The ribosomal exclusion principle for AcPhe-tRNA binding re-examined.
Geigenmüller U, Hausner TP, Nierhaus KH. Geigenmüller U, et al. Eur J Biochem. 1986 Dec 15;161(3):715-21. doi: 10.1111/j.1432-1033.1986.tb10498.x. Eur J Biochem. 1986. PMID: 3024981 - Ribosomal binding of modified tRNA anticodons related to thermal stability.
Ashraf SS, Guenther R, Ye W, Lee Y, Malkiewicz A, Agris PF. Ashraf SS, et al. Nucleic Acids Symp Ser. 1997;(36):58-60. Nucleic Acids Symp Ser. 1997. PMID: 9478206 Review. - Localization of functional centers on the prokaryotic ribosome: immuno-electron microscopy approach.
Spirin AS, Vasiliev VD. Spirin AS, et al. Biol Cell. 1989;66(3):215-23. Biol Cell. 1989. PMID: 2690985 Review.
Cited by
- tRNA Translocation by the Eukaryotic 80S Ribosome and the Impact of GTP Hydrolysis.
Flis J, Holm M, Rundlet EJ, Loerke J, Hilal T, Dabrowski M, Bürger J, Mielke T, Blanchard SC, Spahn CMT, Budkevich TV. Flis J, et al. Cell Rep. 2018 Dec 4;25(10):2676-2688.e7. doi: 10.1016/j.celrep.2018.11.040. Cell Rep. 2018. PMID: 30517857 Free PMC article. - Tracking fluctuation hotspots on the yeast ribosome through the elongation cycle.
Gulay SP, Bista S, Varshney A, Kirmizialtin S, Sanbonmatsu KY, Dinman JD. Gulay SP, et al. Nucleic Acids Res. 2017 May 5;45(8):4958-4971. doi: 10.1093/nar/gkx112. Nucleic Acids Res. 2017. PMID: 28334755 Free PMC article. - Ribosome rescue and translation termination at non-standard stop codons by ICT1 in mammalian mitochondria.
Akabane S, Ueda T, Nierhaus KH, Takeuchi N. Akabane S, et al. PLoS Genet. 2014 Sep 18;10(9):e1004616. doi: 10.1371/journal.pgen.1004616. eCollection 2014 Sep. PLoS Genet. 2014. PMID: 25233460 Free PMC article. - The kissing-loop T-shaped structure translational enhancer of Pea enation mosaic virus can bind simultaneously to ribosomes and a 5' proximal hairpin.
Gao F, Gulay SP, Kasprzak W, Dinman JD, Shapiro BA, Simon AE. Gao F, et al. J Virol. 2013 Nov;87(22):11987-2002. doi: 10.1128/JVI.02005-13. Epub 2013 Aug 28. J Virol. 2013. PMID: 23986599 Free PMC article. - Chromatographic purification of highly active yeast ribosomes.
Meskauskas A, Leshin JA, Dinman JD. Meskauskas A, et al. J Vis Exp. 2011 Oct 24;(56):3214. doi: 10.3791/3214. J Vis Exp. 2011. PMID: 22042245 Free PMC article.