Quantitative assessment of tactile allodynia in the rat paw - PubMed (original) (raw)
Quantitative assessment of tactile allodynia in the rat paw
S R Chaplan et al. J Neurosci Methods. 1994 Jul.
Abstract
We applied and validated a quantitative allodynia assessment technique, using a recently developed rat surgical neuropathy model wherein nocifensive behaviors are evoked by light touch to the paw. Employing von Frey hairs from 0.41 to 15.1 g, we first characterized the percent response at each stimulus intensity. A smooth log-linear relationship was observed, with a median 50% threshold at 1.97 g (95% confidence limits, 1.12-3.57 g). Subsequently, we applied a paradigm using stimulus oscillation around the response threshold, which allowed more rapid, efficient measurements. Median 50% threshold by this up-down method was 2.4 g (1.81-2.76). Correlation coefficient between the two methods was 0.91. In neuropathic rats, good intra- and inter-observer reproducibility was found for the up-down paradigm; some variability was seen in normal rats, attributable to extensive testing. Thresholds in a sizable group of neuropathic rats showed insignificant variability over 20 days. After 50 days, 61% still met strict neuropathy criteria, using survival analysis. Threshold measurement using the up-down paradigm, in combination with the neuropathic pain model, represents a powerful tool for analyzing the effects of manipulations of the neuropathic pain state.
Similar articles
- Effect of spinal cord stimulation in an animal model of neuropathic pain relates to degree of tactile "allodynia".
Smits H, Ultenius C, Deumens R, Koopmans GC, Honig WM, van Kleef M, Linderoth B, Joosten EA. Smits H, et al. Neuroscience. 2006 Dec 1;143(2):541-6. doi: 10.1016/j.neuroscience.2006.08.007. Epub 2006 Sep 15. Neuroscience. 2006. PMID: 16978792 - Comparison of manual and automated filaments for evaluation of neuropathic pain behavior in rats.
Nirogi R, Goura V, Shanmuganathan D, Jayarajan P, Abraham R. Nirogi R, et al. J Pharmacol Toxicol Methods. 2012 Jul;66(1):8-13. doi: 10.1016/j.vascn.2012.04.006. Epub 2012 Apr 30. J Pharmacol Toxicol Methods. 2012. PMID: 22575456 - Topical high molecular weight hyaluronan reduces radicular pain post laminectomy in a rat model.
Massie JB, Schimizzi AL, Huang B, Kim CW, Garfin SR, Akeson WH. Massie JB, et al. Spine J. 2005 Sep-Oct;5(5):494-502. doi: 10.1016/j.spinee.2004.12.003. Spine J. 2005. PMID: 16153575 - Nerve injury-induced changes in opioid modulation of wide dynamic range dorsal column nuclei neurones.
Suzuki R, Dickenson AH. Suzuki R, et al. Neuroscience. 2002;111(1):215-28. doi: 10.1016/s0306-4522(01)00617-0. Neuroscience. 2002. PMID: 11955724 - An analysis of drug interaction between morphine and neostigmine in rats with nerve-ligation injury.
Hwang JH, Hwang KS, Choi Y, Park PH, Han SM, Lee DM. Hwang JH, et al. Anesth Analg. 2000 Feb;90(2):421-6. doi: 10.1097/00000539-200002000-00033. Anesth Analg. 2000. PMID: 10648332
Cited by
- Cortical kappa opioid receptors integrate negative affect and sleep disturbance.
Lillo Vizin RC, Ito H, Kopruszinski CM, Ikegami M, Ikegami D, Yue X, Navratilova E, Moutal A, Cowen SL, Porreca F. Lillo Vizin RC, et al. Transl Psychiatry. 2024 Oct 4;14(1):417. doi: 10.1038/s41398-024-03123-3. Transl Psychiatry. 2024. PMID: 39366962 Free PMC article. - The single-cell transcriptomic atlas iPain identifies senescence of nociceptors as a therapeutical target for chronic pain treatment.
Techameena P, Feng X, Zhang K, Hadjab S. Techameena P, et al. Nat Commun. 2024 Oct 4;15(1):8585. doi: 10.1038/s41467-024-52052-8. Nat Commun. 2024. PMID: 39362841 Free PMC article. - Intraspinal microstimulation of the ventral horn has therapeutically relevant cross-modal effects on nociception.
Bandres MF, Gomes JL, McPherson JG. Bandres MF, et al. Brain Commun. 2024 Aug 19;6(5):fcae280. doi: 10.1093/braincomms/fcae280. eCollection 2024. Brain Commun. 2024. PMID: 39355006 Free PMC article. - Deconstruction the feedforward inhibition changes in the layer III of anterior cingulate cortex after peripheral nerve injury.
Lian YN, Cao XW, Wu C, Pei CY, Liu L, Zhang C, Li XY. Lian YN, et al. Commun Biol. 2024 Oct 1;7(1):1237. doi: 10.1038/s42003-024-06849-4. Commun Biol. 2024. PMID: 39354145 Free PMC article. - BNST CRF receptor type 1 modulates mechanical hypersensitivity induced by adolescent alcohol exposure in adult female mice.
Bertagna NB, Holmgren EB, Engi SA, Ha L, Cruz FC, Albrechet-Souza L, Wills TA. Bertagna NB, et al. Psychopharmacology (Berl). 2024 Sep 30. doi: 10.1007/s00213-024-06693-8. Online ahead of print. Psychopharmacology (Berl). 2024. PMID: 39348004
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous