Groucho is required for Drosophila neurogenesis, segmentation, and sex determination and interacts directly with hairy-related bHLH proteins - PubMed (original) (raw)

Groucho is required for Drosophila neurogenesis, segmentation, and sex determination and interacts directly with hairy-related bHLH proteins

Z Paroush et al. Cell. 1994.

Abstract

We have used the interaction trap, a yeast two-hybrid system, to identify proteins interacting with hairy, a basic-helix-loop-helix (bHLH) protein that represses transcription during Drosophila embryonic segmentation. We find that the groucho (gro) protein binds specifically to hairy and also to hairy-related bHLH proteins encoded by deadpan and the Enhancer of split complex. The C-terminal WRPW motif present in all these bHLH proteins is essential for this interaction. We demonstrate that these associations reflect in vivo maternal requirements for gro during neurogenesis, segmentation, and sex determination, three processes regulated by the above bHLH proteins, and we propose that gro is a transcriptional corepressor recruited to specific target promoters by hairy-related bHLH proteins.

PubMed Disclaimer

Similar articles

• The WRPW motif of the hairy-related basic helix-loop-helix repressor proteins acts as a 4-amino-acid transcription repression and protein-protein interaction domain.
  Fisher AL, Ohsako S, Caudy M. Fisher AL, et al. Mol Cell Biol. 1996 Jun;16(6):2670-7. doi: 10.1128/MCB.16.6.2670. Mol Cell Biol. 1996. PMID: 8649374 Free PMC article.
• Specificity for the hairy/enhancer of split basic helix-loop-helix (bHLH) proteins maps outside the bHLH domain and suggests two separable modes of transcriptional repression.
  Dawson SR, Turner DL, Weintraub H, Parkhurst SM. Dawson SR, et al. Mol Cell Biol. 1995 Dec;15(12):6923-31. doi: 10.1128/MCB.15.12.6923. Mol Cell Biol. 1995. PMID: 8524259 Free PMC article.
• Identification and expression of a novel family of bHLH cDNAs related to Drosophila hairy and enhancer of split.
  Kokubo H, Lun Y, Johnson RL. Kokubo H, et al. Biochem Biophys Res Commun. 1999 Jul 5;260(2):459-65. doi: 10.1006/bbrc.1999.0880. Biochem Biophys Res Commun. 1999. PMID: 10403790
• Expression and function of Enhancer of split bHLH proteins during Drosophila neurogenesis.
  Bray SJ. Bray SJ. Perspect Dev Neurobiol. 1997;4(4):313-23. Perspect Dev Neurobiol. 1997. PMID: 9171445 Review.

Cited by

• Recent insights into Groucho co-repressor recruitment and function.
  Kaul AK, Schuster EF, Jennings BH. Kaul AK, et al. Transcription. 2015;6(1):7-11. doi: 10.1080/21541264.2014.1000709. Epub 2015 Jan 20. Transcription. 2015. PMID: 25603281 Free PMC article.
• The notch pathway: modulation of cell fate decisions in hematopoiesis.
  Ohishi K, Varnum-Finney B, Bernstein ID. Ohishi K, et al. Int J Hematol. 2002 Jun;75(5):449-59. doi: 10.1007/BF02982106. Int J Hematol. 2002. PMID: 12095143 Review.
• A new Groucho TLE4 protein may regulate the repressive activity of Pax5 in human B lymphocytes.
  Milili M, Gauthier L, Veran J, Mattei MG, Schiff C. Milili M, et al. Immunology. 2002 Aug;106(4):447-55. doi: 10.1046/j.1365-2567.2002.01456.x. Immunology. 2002. PMID: 12153506 Free PMC article.
• Brinker requires two corepressors for maximal and versatile repression in Dpp signalling.
  Hasson P, Müller B, Basler K, Paroush Z. Hasson P, et al. EMBO J. 2001 Oct 15;20(20):5725-36. doi: 10.1093/emboj/20.20.5725. EMBO J. 2001. PMID: 11598015 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • FlyBase
  • GlyGen glycoinformatics resource