Three additional linkage groups that repress transcription and meiotic recombination in the mating-type region of Schizosaccharomyces pombe - PubMed (original) (raw)
Three additional linkage groups that repress transcription and meiotic recombination in the mating-type region of Schizosaccharomyces pombe
G Thon et al. Genetics. 1994 Sep.
Abstract
The mating-type genes of Schizosaccharomyces pombe are found at three locations in the same chromosomal region. These genes are in an active configuration at the mat1 locus and in an inactive configuration at the mat2 and mat3 loci. The mechanism that represses transcription of mat2 and mat3 also inactivates other promoters introduced nearby and is accompanied by a block to meiotic recombination in the mat2-mat3 interval, suggesting that this mechanism involves a particular chromatin structure. We present evidence that the transcription and recombination blocks require three newly defined trans-acting loci, clr2, clr3 and clr4, in addition to the previously identified clr1, rik1 and swi6 loci. We also investigated the role of mat2 cis-acting sequences in silencing. Four cis-acting elements that repress mat2 in a plasmid context were previously identified. Deletion of two of these elements proved to have little effect in a chromosomal context. However, when combined with mutations in trans-acting genes, deletion of the same two elements greatly enhanced mat2 expression. The observed cumulative effects suggest a redundancy in the silencing mechanism.
Similar articles
- The clr1 locus regulates the expression of the cryptic mating-type loci of fission yeast.
Thon G, Klar AJ. Thon G, et al. Genetics. 1992 Jun;131(2):287-96. doi: 10.1093/genetics/131.2.287. Genetics. 1992. PMID: 1644273 Free PMC article. - Mutations in rik1, clr2, clr3 and clr4 genes asymmetrically derepress the silent mating-type loci in fission yeast.
Ekwall K, Ruusala T. Ekwall K, et al. Genetics. 1994 Jan;136(1):53-64. doi: 10.1093/genetics/136.1.53. Genetics. 1994. PMID: 8138176 Free PMC article. - Localization and properties of a silencing element near the mat3-M mating-type cassette of Schizosaccharomyces pombe.
Thon G, Bjerling KP, Nielsen IS. Thon G, et al. Genetics. 1999 Mar;151(3):945-63. doi: 10.1093/genetics/151.3.945. Genetics. 1999. PMID: 10049914 Free PMC article. - Transcriptional silencing in fission yeast.
Grewal SI. Grewal SI. J Cell Physiol. 2000 Sep;184(3):311-8. doi: 10.1002/1097-4652(200009)184:3<311::AID-JCP4>3.0.CO;2-D. J Cell Physiol. 2000. PMID: 10911361 Review. - Multiple epigenetic events regulate mating-type switching of fission yeast.
Klar AJ, Ivanova AV, Dalgaard JZ, Bonaduce MJ, Grewal SI. Klar AJ, et al. Novartis Found Symp. 1998;214:87-99; discussion 99-103. doi: 10.1002/9780470515501.ch6. Novartis Found Symp. 1998. PMID: 9601013 Review.
Cited by
- The fork protection complex promotes parental histone recycling and epigenetic memory.
Charlton SJ, Flury V, Kanoh Y, Genzor AV, Kollenstart L, Ao W, Brøgger P, Weisser MB, Adamus M, Alcaraz N, Delvaux de Fenffe CM, Mattiroli F, Montoya G, Masai H, Groth A, Thon G. Charlton SJ, et al. Cell. 2024 Sep 5;187(18):5029-5047.e21. doi: 10.1016/j.cell.2024.07.017. Epub 2024 Aug 1. Cell. 2024. PMID: 39094569 Free PMC article. - Mathematical model for the role of multiple pericentromeric repeats on heterochromatin assembly.
Ghimire P, Motamedi M, Joh R. Ghimire P, et al. PLoS Comput Biol. 2024 Apr 10;20(4):e1012027. doi: 10.1371/journal.pcbi.1012027. eCollection 2024 Apr. PLoS Comput Biol. 2024. PMID: 38598558 Free PMC article. - Specialized replication of heterochromatin domains ensures self-templated chromatin assembly and epigenetic inheritance.
Nathanailidou P, Dhakshnamoorthy J, Xiao H, Zofall M, Holla S, O'Neill M, Andresson T, Wheeler D, Grewal SIS. Nathanailidou P, et al. Proc Natl Acad Sci U S A. 2024 Feb 6;121(6):e2315596121. doi: 10.1073/pnas.2315596121. Epub 2024 Jan 29. Proc Natl Acad Sci U S A. 2024. PMID: 38285941 Free PMC article. - The Fission Yeast Mating-Type Switching Motto: "One-for-Two" and "Two-for-One".
Arcangioli B, Gangloff S. Arcangioli B, et al. Microbiol Mol Biol Rev. 2023 Mar 21;87(1):e0000821. doi: 10.1128/mmbr.00008-21. Epub 2023 Jan 11. Microbiol Mol Biol Rev. 2023. PMID: 36629411 Free PMC article. Review. - Histone deacetylation primes self-propagation of heterochromatin domains to promote epigenetic inheritance.
Zofall M, Sandhu R, Holla S, Wheeler D, Grewal SIS. Zofall M, et al. Nat Struct Mol Biol. 2022 Sep;29(9):898-909. doi: 10.1038/s41594-022-00830-7. Epub 2022 Sep 5. Nat Struct Mol Biol. 2022. PMID: 36064597 Free PMC article.
References
- EMBO J. 1991 Oct;10(10):3025-32 - PubMed
- Trends Genet. 1990 Dec;6(12):422-6 - PubMed
- Genetics. 1991 Dec;129(4):1033-42 - PubMed
- Mol Gen Genet. 1992 Jun;233(3):436-42 - PubMed
- Curr Genet. 1992 Apr;21(4-5):331-8 - PubMed
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Molecular Biology Databases