Cadherin function is required for human keratinocytes to assemble desmosomes and stratify in response to calcium - PubMed (original) (raw)
Cadherin function is required for human keratinocytes to assemble desmosomes and stratify in response to calcium
J E Lewis et al. J Invest Dermatol. 1994 Jun.
Free article
Abstract
Elevation of the calcium concentration in keratinocyte culture induces the rapid organization of adherens junctions and desmosomes. Formation of these intercellular junctions is accompanied by reorganization of the cytoskeleton and, with a more delayed timecourse, stratification of the culture into a multilayered epithelial cell sheet. Keratinocytes express two cadherins, known as E- and P-cadherin, which are the cell-cell adhesion molecules of the adherens junction. Antibody that blocks E-cadherin function delays the calcium-induced formation of both adherens junctions and desmosomes and leads to an abnormally stratified cultured. In the present study, we show that anti-E-cadherin plus anti-P-cadherin antibodies inhibit the formation of adherens junctions and desmosomes, prevent reorganization of the cytoskeleton, and block stratification. These studies indicate that cadherin function is required for the calcium-induced intercellular junction organization and stratification of human keratinocytes in vitro.
Similar articles
- 1,25-dihydroxyvitamin D3 stimulates the assembly of adherens junctions in keratinocytes: involvement of protein kinase C.
Gniadecki R, Gajkowska B, Hansen M. Gniadecki R, et al. Endocrinology. 1997 Jun;138(6):2241-8. doi: 10.1210/endo.138.6.5156. Endocrinology. 1997. PMID: 9165007 - Cross-talk between adherens junctions and desmosomes depends on plakoglobin.
Lewis JE, Wahl JK 3rd, Sass KM, Jensen PJ, Johnson KR, Wheelock MJ. Lewis JE, et al. J Cell Biol. 1997 Feb 24;136(4):919-34. doi: 10.1083/jcb.136.4.919. J Cell Biol. 1997. PMID: 9049256 Free PMC article. - Regulation of keratinocyte intercellular junction organization and epidermal morphogenesis by E-cadherin.
Wheelock MJ, Jensen PJ. Wheelock MJ, et al. J Cell Biol. 1992 Apr;117(2):415-25. doi: 10.1083/jcb.117.2.415. J Cell Biol. 1992. PMID: 1373144 Free PMC article. - Analysis of desmosomal cadherin-adhesive function and stoichiometry of desmosomal cadherin-plakoglobin complexes.
Kowalczyk AP, Borgwardt JE, Green KJ. Kowalczyk AP, et al. J Invest Dermatol. 1996 Sep;107(3):293-300. doi: 10.1111/1523-1747.ep12363000. J Invest Dermatol. 1996. PMID: 8751959 Review. - Roles of E- and P-cadherin in the human skin.
Furukawa F, Fujii K, Horiguchi Y, Matsuyoshi N, Fujita M, Toda K, Imamura S, Wakita H, Shirahama S, Takigawa M. Furukawa F, et al. Microsc Res Tech. 1997 Aug 15;38(4):343-52. doi: 10.1002/(SICI)1097-0029(19970815)38:4<343::AID-JEMT2>3.0.CO;2-K. Microsc Res Tech. 1997. PMID: 9297684 Review.
Cited by
- Immortalized Human hTert/KER-CT Keratinocytes a Model System for Research on Desmosomal Adhesion and Pathogenesis of Pemphigus Vulgaris.
Beckert B, Panico F, Pollmann R, Eming R, Banning A, Tikkanen R. Beckert B, et al. Int J Mol Sci. 2019 Jun 26;20(13):3113. doi: 10.3390/ijms20133113. Int J Mol Sci. 2019. PMID: 31247885 Free PMC article. - Protein kinase C activation upregulates intercellular adhesion of alpha-catenin-negative human colon cancer cell variants via induction of desmosomes.
van Hengel J, Gohon L, Bruyneel E, Vermeulen S, Cornelissen M, Mareel M, von Roy F. van Hengel J, et al. J Cell Biol. 1997 Jun 2;137(5):1103-16. doi: 10.1083/jcb.137.5.1103. J Cell Biol. 1997. PMID: 9166410 Free PMC article. - Altered epidermal proliferation, differentiation, and lipid composition: Novel key elements in the vitiligo puzzle.
Kovacs D, Bastonini E, Briganti S, Ottaviani M, D'Arino A, Truglio M, Sciuto L, Zaccarini M, Pacifico A, Cota C, Iacovelli P, Picardo M. Kovacs D, et al. Sci Adv. 2022 Sep 2;8(35):eabn9299. doi: 10.1126/sciadv.abn9299. Epub 2022 Sep 2. Sci Adv. 2022. PMID: 36054352 Free PMC article. - JNK inhibition enhances cell-cell adhesion impaired by desmoglein 3 gene disruption in keratinocytes.
Ogawa S, Ishii T, Otani T, Inai Y, Matsuura T, Inai T. Ogawa S, et al. Histochem Cell Biol. 2024 Apr;161(4):345-357. doi: 10.1007/s00418-023-02264-8. Epub 2024 Jan 16. Histochem Cell Biol. 2024. PMID: 38227055 - Catenins: keeping cells from getting their signals crossed.
Perez-Moreno M, Fuchs E. Perez-Moreno M, et al. Dev Cell. 2006 Nov;11(5):601-12. doi: 10.1016/j.devcel.2006.10.010. Dev Cell. 2006. PMID: 17084354 Free PMC article. Review.