Cytosolic ARFs are required for vesicle formation but not for cell-free intra-Golgi transport: evidence for coated vesicle-independent transport - PubMed (original) (raw)

Cytosolic ARFs are required for vesicle formation but not for cell-free intra-Golgi transport: evidence for coated vesicle-independent transport

T C Taylor et al. Mol Biol Cell. 1994 Feb.

Free PMC article

Abstract

We investigated the role of ADP-ribosylation factors (ARFs) in Golgi function using biochemical and morphological cell-free assays. An ARF-free cytosol produced from soluble Chinese hamster ovary (CHO) extracts supports intra-Golgi transport by a mechanism that is biochemically indistinguishable from control transport reactions: ARF-free transport reactions are NSF-dependent, remain sensitive to the donor Golgi-specific inhibitor ilimaquinone, and exhibit kinetics that are identical to that of control reactions containing ARFs. In contrast, ARF-free cytosol does not support the formation of coated vesicles on Golgi cisternae. However, vesicle formation is reconstituted upon the addition of ARF1. These data suggest that neither soluble ARFs nor coated vesicle formation are essential for transport. We conclude that cell-free intra-Golgi transport proceeds via a coated vesicle-independent mechanism regardless of vesicle formation on Golgi cisternae.

PubMed Disclaimer

Cited by

Poliovirus proteins induce membrane association of GTPase ADP-ribosylation factor.
Belov GA, Fogg MH, Ehrenfeld E. Belov GA, et al. J Virol. 2005 Jun;79(11):7207-16. doi: 10.1128/JVI.79.11.7207-7216.2005. J Virol. 2005. PMID: 15890959 Free PMC article.
ER/Golgi intermediates acquire Golgi enzymes by brefeldin A-sensitive retrograde transport in vitro.
Lin CC, Love HD, Gushue JN, Bergeron JJ, Ostermann J. Lin CC, et al. J Cell Biol. 1999 Dec 27;147(7):1457-72. doi: 10.1083/jcb.147.7.1457. J Cell Biol. 1999. PMID: 10613904 Free PMC article.
Roles for alpha(2)p24 and COPI in endoplasmic reticulum cargo exit site formation.
Lavoie C, Paiement J, Dominguez M, Roy L, Dahan S, Gushue JN, Bergeron JJ. Lavoie C, et al. J Cell Biol. 1999 Jul 26;146(2):285-99. doi: 10.1083/jcb.146.2.285. J Cell Biol. 1999. PMID: 10427085 Free PMC article.
Fusogenic domains of golgi membranes are sequestered into specialized regions of the stack that can be released by mechanical fragmentation.
Dominguez M, Fazel A, Dahan S, Lovell J, Hermo L, Claude A, Melançon P, Bergeron JJ. Dominguez M, et al. J Cell Biol. 1999 May 17;145(4):673-88. doi: 10.1083/jcb.145.4.673. J Cell Biol. 1999. PMID: 10330398 Free PMC article.
The molecular characterization of transport vesicles.
Robinson DG, Hinz G, Holstein SE. Robinson DG, et al. Plant Mol Biol. 1998 Sep;38(1-2):49-76. Plant Mol Biol. 1998. PMID: 9738960 Review.

References

1. J Cell Biol. 1992 Sep;118(5):1015-26 - PubMed
1. Cell. 1993 Nov 19;75(4):593-6 - PubMed
1. Cell. 1989 Feb 10;56(3):357-68 - PubMed
1. Nature. 1993 Mar 25;362(6418):297-8 - PubMed
1. Cell. 1984 Dec;39(2 Pt 1):405-16 - PubMed

Cytosolic ARFs are required for vesicle formation but not for cell-free intra-Golgi transport: evidence for coated vesicle-independent transport - PubMed (original) (raw)