Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types - PubMed (original) (raw)

Comparative Study

Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types

W M Bement et al. Proc Natl Acad Sci U S A. 1994.

Erratum in

• Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types.
  Bement WM, Hasson T, Wirth JA, Cheney RE, Mooseker MS. Bement WM, et al. Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11767. doi: 10.1073/pnas.91.24.11767-c. Proc Natl Acad Sci U S A. 1994. PMID: 7972138 Free PMC article. No abstract available.

Abstract

Myosin diversity in the human epithelial cell line Caco-2BBe, the porcine epithelial cell line LLC-PK1 (CL-4), human peripheral blood leukocytes, and human liver was analyzed. PCR amplification yielded 8-11 putative myosins (depending on the cDNA source) representing six distinct myosin classes. Analysis of clones obtained by hybridization screening demonstrated that the original PCR products correspond to bona fide myosins, based on the presence of sequences highly conserved in other myosins. RNase protection analysis confirmed mRNA expression of 11 myosins in Caco-2BBe cells. Immunoblot analysis showed that at least 6 myosin immunogens are expressed in Caco-2BBe cells. The results reveal the existence of at least 11 unconventional human myosin genes, most of which are expressed in an overlapping fashion in different cell types. The abundance of myosins suggests that the myosin I vs. myosin II paradigm is inadequate to explain actin-based cellular motility.

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References

1. 1. Nature. 1989 Sep 28;341(6240):328-31 - PubMed
2. 1. Annu Rev Cell Biol. 1987;3:379-421 - PubMed
3. 1. Nature. 1991 Feb 21;349(6311):709-13 - PubMed
4. 1. Proc Natl Acad Sci U S A. 1989 Aug;86(16):6186-90 - PubMed
5. 1. J Immunol Methods. 1974 Aug;5(3):249-52 - PubMed

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