Purification of a sixth ferredoxin from Rhodobacter capsulatus. Primary structure and biochemical properties - PubMed (original) (raw)

Comparative Study

Purification of a sixth ferredoxin from Rhodobacter capsulatus. Primary structure and biochemical properties

I Naud et al. Eur J Biochem. 1994.

Free article

Abstract

A new ferredoxin has been purified from the photosynthetic bacterium Rhodobacter capsulatus. It is the sixth ferredoxin to be isolated from this bacterium and it was called FdVI. Its primary structure was established based on amino acid sequence analysis of the protein and of peptides derived from it. It is composed of 106 residues including five cysteines. The calculated mass of the polypeptide is 11,402.6 Da which matches the experimental value determined by electrospray mass spectrometry. Amino acid sequence comparison revealed that ferredoxin VI (FdVI) is strikingly similar to a ferredoxin from Caulobacter crescentus and to the putidaredoxin from Pseudomonas putida. FdVI exhibited an ultraviolet-visible absorption spectrum typical for a [2Fe-2S] ferredoxin. EPR spectroscopy of the reduced protein showed a nearly axial signal similar to that of mitochondrial and P. putida ferredoxins. FdVI is biosynthesized in cells growing anaerobically under either nitrogen-sufficient or nitrogen-deficient conditions. Although the function of FdVI is unknown, its structural resemblance to [2Fe-2S] ferredoxins known to transfer electrons to oxygenases such as P-450 cytochromes, suggests that FdVI may have a similar role in R. capsulatus.

PubMed Disclaimer

Similar articles

• Structure of a [2Fe-2S] ferredoxin from Rhodobacter capsulatus likely involved in Fe-S cluster biogenesis and conformational changes observed upon reduction.
  Sainz G, Jakoncic J, Sieker LC, Stojanoff V, Sanishvili N, Asso M, Bertrand P, Armengaud J, Jouanneau Y. Sainz G, et al. J Biol Inorg Chem. 2006 Mar;11(2):235-46. doi: 10.1007/s00775-005-0069-2. Epub 2006 Jan 10. J Biol Inorg Chem. 2006. PMID: 16402206
• Crystallization and preliminary X-ray diffraction analysis of a [2Fe-2S] ferredoxin (FdVI) from Rhodobacter capsulatus.
  Armengaud J, Sainz G, Jouanneau Y, Sieker LC. Armengaud J, et al. Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):301-3. doi: 10.1107/s0907444900017832. Acta Crystallogr D Biol Crystallogr. 2001. PMID: 11173487
• Purification and characterization of a novel dimeric ferredoxin (FdIII) from Rhodobacter capsulatus.
  Jouanneau Y, Meyer C, Gaillard J, Forest E, Gagnon J. Jouanneau Y, et al. J Biol Chem. 1993 May 15;268(14):10636-44. J Biol Chem. 1993. PMID: 8387524
• Eukaryotic genomes contain a [2Fez.sbnd;2S] ferredoxin isoform with a conserved C-terminal sequence motif.
  Seeber F. Seeber F. Trends Biochem Sci. 2002 Nov;27(11):545-7. doi: 10.1016/s0968-0004(02)02196-5. Trends Biochem Sci. 2002. PMID: 12417122 Review.
• A ferredoxin-like domain in RNA polymerase 30/40-kDa subunits.
  Rodriguez-Monge L, Ouzounis CA, Kyrpides NC. Rodriguez-Monge L, et al. Trends Biochem Sci. 1998 May;23(5):169-70. doi: 10.1016/s0968-0004(98)01203-1. Trends Biochem Sci. 1998. PMID: 9612079 Review. No abstract available.

Cited by

• Structure of a [2Fe-2S] ferredoxin from Rhodobacter capsulatus likely involved in Fe-S cluster biogenesis and conformational changes observed upon reduction.
  Sainz G, Jakoncic J, Sieker LC, Stojanoff V, Sanishvili N, Asso M, Bertrand P, Armengaud J, Jouanneau Y. Sainz G, et al. J Biol Inorg Chem. 2006 Mar;11(2):235-46. doi: 10.1007/s00775-005-0069-2. Epub 2006 Jan 10. J Biol Inorg Chem. 2006. PMID: 16402206
• Redox chains in chloroplast envelope membranes: spectroscopic evidence for the presence of electron carriers, including iron-sulfur centers.
  Jäger-Vottero P, Dorne AJ, Jordanov J, Douce R, Joyard J. Jäger-Vottero P, et al. Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1597-602. doi: 10.1073/pnas.94.4.1597. Proc Natl Acad Sci U S A. 1997. PMID: 11038604 Free PMC article.
• Evidence for the bacterial origin of genes encoding fermentation enzymes of the amitochondriate protozoan parasite Entamoeba histolytica.
  Rosenthal B, Mai Z, Caplivski D, Ghosh S, de la Vega H, Graf T, Samuelson J. Rosenthal B, et al. J Bacteriol. 1997 Jun;179(11):3736-45. doi: 10.1128/jb.179.11.3736-3745.1997. J Bacteriol. 1997. PMID: 9171424 Free PMC article.
• A [2Fe-2S] ferredoxin (FdVI) is essential for growth of the photosynthetic bacterium Rhodobacter capsulatus.
  Armengaud J, Meyer C, Jouanneau Y. Armengaud J, et al. J Bacteriol. 1997 May;179(10):3304-9. doi: 10.1128/jb.179.10.3304-3309.1997. J Bacteriol. 1997. PMID: 9150228 Free PMC article.
• A ferredoxin, designated FdxP, stimulates p-hydroxybenzoate hydroxylase activity in Caulobacter crescentus.
  Wang SP, Chen YP, Ely B. Wang SP, et al. J Bacteriol. 1995 May;177(10):2908-11. doi: 10.1128/jb.177.10.2908-2911.1995. J Bacteriol. 1995. PMID: 7751304 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley