Retrograde transport from the Golgi complex to the ER of both Shiga toxin and the nontoxic Shiga B-fragment is regulated by butyric acid and cAMP - PubMed (original) (raw)

Comparative Study

Retrograde transport from the Golgi complex to the ER of both Shiga toxin and the nontoxic Shiga B-fragment is regulated by butyric acid and cAMP

K Sandvig et al. J Cell Biol. 1994 Jul.

Abstract

Endocytosed Shiga toxin is transported from the Golgi complex to the endoplasmic reticulum in butyric acid-treated A431 cells. We here examine the extent of this retrograde transport and its regulation. The short B fragment of Shiga toxin is sufficient for transport to the ER. The B fragment of cholera toxin, which also binds to glycolipids, is transported to all the Golgi cisterns, but cannot be localized in the ER even after butyric acid treatment. Under all conditions the toxic protein ricin was found predominantly in the trans-Golgi network. There is no transport of endocytosed fluid to the Golgi apparatus or to the ER even after butyric acid treatment and in the presence of Shiga toxin, indicating that transport to the ER, through the trans-Golgi network and the cisterns of the Golgi apparatus, involves several sorting stations. Since Shiga toxin receptors (Gb3) in butyric acid-treated A431 cells seem to have a ceramide moiety with longer fatty acids than in untreated cells, the possibility exists that fatty acid composition of the receptor is important for sorting to the ER. Both retrograde transport and intoxication with Shiga toxin can also be induced by cAMP, supporting the idea that retrograde transport from the Golgi to the ER is required for intoxication. The data suggest that transport to the ER in cells in situ may depend on fatty acid composition and is regulated by physiological signals.

PubMed Disclaimer

Cited by

Sortase-Modified Cholera Toxoids Show Specific Golgi Localization.
Machin DC, Williamson DJ, Fisher P, Miller VJ, Arnott ZLP, Stevenson CME, Wildsmith GC, Ross JF, Wasson CW, Macdonald A, Andrews BI, Ungar D, Turnbull WB, Webb ME. Machin DC, et al. Toxins (Basel). 2024 Apr 16;16(4):194. doi: 10.3390/toxins16040194. Toxins (Basel). 2024. PMID: 38668619 Free PMC article.
The Mutagenic Plasticity of the Cholera Toxin B-Subunit Surface Residues: Stability and Affinity.
Au CW, Manfield I, Webb ME, Paci E, Turnbull WB, Ross JF. Au CW, et al. Toxins (Basel). 2024 Mar 4;16(3):133. doi: 10.3390/toxins16030133. Toxins (Basel). 2024. PMID: 38535799 Free PMC article.
Cholera Toxin as a Probe for Membrane Biology.
Kenworthy AK, Schmieder SS, Raghunathan K, Tiwari A, Wang T, Kelly CV, Lencer WI. Kenworthy AK, et al. Toxins (Basel). 2021 Aug 3;13(8):543. doi: 10.3390/toxins13080543. Toxins (Basel). 2021. PMID: 34437414 Free PMC article. Review.
Combined Action of Shiga Toxin Type 2 and Subtilase Cytotoxin in the Pathogenesis of Hemolytic Uremic Syndrome.
Álvarez RS, Gómez FD, Zotta E, Paton AW, Paton JC, Ibarra C, Sacerdoti F, Amaral MM. Álvarez RS, et al. Toxins (Basel). 2021 Jul 29;13(8):536. doi: 10.3390/toxins13080536. Toxins (Basel). 2021. PMID: 34437406 Free PMC article.
The Gb3-enriched CD59/flotillin plasma membrane domain regulates host cell invasion by Pseudomonas aeruginosa.
Brandel A, Aigal S, Lagies S, Schlimpert M, Meléndez AV, Xu M, Lehmann A, Hummel D, Fisch D, Madl J, Eierhoff T, Kammerer B, Römer W. Brandel A, et al. Cell Mol Life Sci. 2021 Apr;78(7):3637-3656. doi: 10.1007/s00018-021-03766-1. Epub 2021 Feb 8. Cell Mol Life Sci. 2021. PMID: 33555391 Free PMC article.

References

1. Nature. 1993 Aug 26;364(6440):818-21 - PubMed
1. J Biol Chem. 1991 Jan 5;266(1):484-9 - PubMed
1. FEBS Lett. 1988 May 9;232(1):193-8 - PubMed
1. Arch Biochem Biophys. 1976 Feb;172(2):618-26 - PubMed
1. Methods Enzymol. 1978;50:330-5 - PubMed

Retrograde transport from the Golgi complex to the ER of both Shiga toxin and the nontoxic Shiga B-fragment is regulated by butyric acid and cAMP - PubMed (original) (raw)