"Inside-out" signal transduction inhibited by isolated integrin cytoplasmic domains - PubMed (original) (raw)
. 1994 Jul 15;269(28):18307-10.
Affiliations
- PMID: 8034576
Free article
"Inside-out" signal transduction inhibited by isolated integrin cytoplasmic domains
Y P Chen et al. J Biol Chem. 1994.
Free article
Abstract
The affinities of integrin alpha beta heterodimers for extracellular ligands are important regulators of cell adhesion. Intracellular signals provoke changes in the integrin extracellular domain resulting in "activation," as manifested by an increase in affinity. Interactions of integrin cytoplasmic domains with intracellular elements may mediate this "inside-out signaling." Here we report that overexpression of chimeras of the cytoplasmic domain of integrin beta 3 or beta 1 subunits, joined to the extracellular and transmembrane domains of the Tac subunit of the interleukin-2 receptor, reduced integrin affinity. In contrast, chimeras containing the cytoplasmic domain of alpha 5 or alpha IIb or of beta 3 bearing a mutation that disrupts inside-out signaling lacked inhibitory activity. These data suggest that limiting quantities of intracellular factors bind to integrin beta 3 and beta 1 cytoplasmic domains to modulate ligand binding affinity. Structural mimics of these domains may provide a novel means to alter cell adhesion.
Similar articles
- Integrin cytoplasmic domains mediate inside-out signal transduction.
O'Toole TE, Katagiri Y, Faull RJ, Peter K, Tamura R, Quaranta V, Loftus JC, Shattil SJ, Ginsberg MH. O'Toole TE, et al. J Cell Biol. 1994 Mar;124(6):1047-59. doi: 10.1083/jcb.124.6.1047. J Cell Biol. 1994. PMID: 7510712 Free PMC article. - Modulation of cell adhesion by changes in alpha L beta 2 (LFA-1, CD11a/CD18) cytoplasmic domain/cytoskeleton interaction.
Peter K, O'Toole TE. Peter K, et al. J Exp Med. 1995 Jan 1;181(1):315-26. doi: 10.1084/jem.181.1.315. J Exp Med. 1995. PMID: 7807011 Free PMC article. - Distinct functions of integrin alpha and beta subunit cytoplasmic domains in cell spreading and formation of focal adhesions.
Ylänne J, Chen Y, O'Toole TE, Loftus JC, Takada Y, Ginsberg MH. Ylänne J, et al. J Cell Biol. 1993 Jul;122(1):223-33. doi: 10.1083/jcb.122.1.223. J Cell Biol. 1993. PMID: 8314843 Free PMC article. - Integrins in cell adhesion and signaling.
Akiyama SK. Akiyama SK. Hum Cell. 1996 Sep;9(3):181-6. Hum Cell. 1996. PMID: 9183647 Review. - Integrin cytoplasmic domains: mediators of cytoskeletal linkages and extra- and intracellular initiated transmembrane signaling.
Sastry SK, Horwitz AF. Sastry SK, et al. Curr Opin Cell Biol. 1993 Oct;5(5):819-31. doi: 10.1016/0955-0674(93)90031-k. Curr Opin Cell Biol. 1993. PMID: 8240826 Review.
Cited by
- Gα13 Switch Region 2 Relieves Talin Autoinhibition to Activate αIIbβ3 Integrin.
Schiemer J, Bohm A, Lin L, Merrill-Skoloff G, Flaumenhaft R, Huang JS, Le Breton GC, Chishti AH. Schiemer J, et al. J Biol Chem. 2016 Dec 23;291(52):26598-26612. doi: 10.1074/jbc.M116.747279. Epub 2016 Nov 1. J Biol Chem. 2016. PMID: 27803165 Free PMC article. - Roles of integrin β3 cytoplasmic tail in bidirectional signal transduction in a trans-dominant inhibition model.
Huang J, Zhou Y, Su X, Lyu Y, Tao L, Shi X, Liu P, Long Z, Ruan Z, Xiao B, Xi W, Zhou Q, Mao J, Xi X. Huang J, et al. Front Med. 2016 Sep;10(3):311-9. doi: 10.1007/s11684-016-0460-0. Epub 2016 Sep 7. Front Med. 2016. PMID: 27527361 - Evaluation of targeting c-Src by the RGT-containing peptide as a novel antithrombotic strategy.
Huang J, Shi X, Xi W, Liu P, Long Z, Xi X. Huang J, et al. J Hematol Oncol. 2015 May 30;8:62. doi: 10.1186/s13045-015-0159-8. J Hematol Oncol. 2015. PMID: 26025329 Free PMC article. - Headpiece domain of dematin regulates calcium mobilization and signaling in platelets.
Wieschhaus AJ, Le Breton GC, Chishti AH. Wieschhaus AJ, et al. J Biol Chem. 2012 Nov 30;287(49):41218-31. doi: 10.1074/jbc.M112.364679. Epub 2012 Oct 11. J Biol Chem. 2012. PMID: 23060452 Free PMC article. - SHARPIN is an endogenous inhibitor of β1-integrin activation.
Rantala JK, Pouwels J, Pellinen T, Veltel S, Laasola P, Mattila E, Potter CS, Duffy T, Sundberg JP, Kallioniemi O, Askari JA, Humphries MJ, Parsons M, Salmi M, Ivaska J. Rantala JK, et al. Nat Cell Biol. 2011 Sep 25;13(11):1315-24. doi: 10.1038/ncb2340. Nat Cell Biol. 2011. PMID: 21947080 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources