Quantitative analysis of the contribution made by 5'-flanking and 3'-flanking sequences to the transcriptional regulation of junB by growth factors - PubMed (original) (raw)
Affiliations
- PMID: 8036018
Quantitative analysis of the contribution made by 5'-flanking and 3'-flanking sequences to the transcriptional regulation of junB by growth factors
D G Phinney et al. Oncogene. 1994 Aug.
Abstract
We have identified four DNAase I-hypersensitive regions (DHRs) at the junB locus. DHR1 is located between sequences -100 and +250, DHR2 is centered at -1000, DHR3 at -1650, and DHR4 at +2040 relative to the junB transcriptional start site. Sequence analysis of these DHRs revealed two serum response elements at -1452 and +2091, two cyclic AMP response elements at +2071 and +2116, and a 12-O-tetradecanoylphorbol-13-acetate (TPA) response element at -949. To study the contribution made by these cis-elements to junB transcriptional regulation, we stably transfected a recombinant mouse junB gene (JBSV4) containing the intact junB coding sequences, 6.3 kb of 5'-flanking DNA, and 2.0 kb of 3'-flanking DNA into Rat1A cells. The pattern of DHRs identified at the mouse junB locus was re-established at the JBSV4 locus. By directly comparing JBSV4 and rat junB mRNA levels, we found that these genes were induced to equivalent levels by serum, TPA, cyclic AMP, platelet-derived growth factor, epidermal growth factor, and basic fibroblastic growth factor. These results established that JBSV4 resides in a physical environment within chromatin that closely mimics that of the junB locus, and contains the necessary sequence information to recapitulate the transcriptional regulation of junB. By analysing a series of recombinant mouse junB genes containing deletion mutations in 5'-flanking and 3'-flanking sequences, we provide a quantitative assessment of the contribution these sequences make to junB induction by different regulatory agents.
Similar articles
- Successive occupancy by immediate early transcriptional factors of the tyrosine hydroxylase gene TRE and CRE sites in PACAP-stimulated PC12 pheochromocytoma cells.
Yukimasa N, Isobe K, Nagai H, Takuwa Y, Nakai T. Yukimasa N, et al. Neuropeptides. 1999 Dec;33(6):475-82. doi: 10.1054/npep.1999.0765. Neuropeptides. 1999. PMID: 10657527 - Transcriptional control of the platelet-derived growth factor subunit genes.
Kaetzel DM, Coyne DW, Fenstermaker RA. Kaetzel DM, et al. Biofactors. 1993 May;4(2):71-81. Biofactors. 1993. PMID: 8347277 Review. - The tis genes, primary response genes induced by growth factors and tumor promoters in 3T3 cells.
Herschman HR, Kujubu DA, Fletcher BS, Ma Q, Varnum BC, Gilbert RS, Reddy ST. Herschman HR, et al. Prog Nucleic Acid Res Mol Biol. 1994;47:113-48. doi: 10.1016/s0079-6603(08)60251-2. Prog Nucleic Acid Res Mol Biol. 1994. PMID: 8016319 Review. No abstract available.
Cited by
- The Phosphorylation of CREB at Serine 133 Is a Key Event for Circadian Clock Timing and Entrainment in the Suprachiasmatic Nucleus.
Wheaton KL, Hansen KF, Aten S, Sullivan KA, Yoon H, Hoyt KR, Obrietan K. Wheaton KL, et al. J Biol Rhythms. 2018 Oct;33(5):497-514. doi: 10.1177/0748730418791713. Epub 2018 Sep 3. J Biol Rhythms. 2018. PMID: 30175684 Free PMC article. - The natural chemopreventive agent sulforaphane inhibits STAT5 activity.
Pinz S, Unser S, Rascle A. Pinz S, et al. PLoS One. 2014 Jun 9;9(6):e99391. doi: 10.1371/journal.pone.0099391. eCollection 2014. PLoS One. 2014. PMID: 24910998 Free PMC article. - Transcriptional pausing caused by NELF plays a dual role in regulating immediate-early expression of the junB gene.
Aida M, Chen Y, Nakajima K, Yamaguchi Y, Wada T, Handa H. Aida M, et al. Mol Cell Biol. 2006 Aug;26(16):6094-104. doi: 10.1128/MCB.02366-05. Mol Cell Biol. 2006. PMID: 16880520 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Molecular Biology Databases