DNA fingerprinting of medically important microorganisms by use of PCR - PubMed (original) (raw)

Review

DNA fingerprinting of medically important microorganisms by use of PCR

A van Belkum. Clin Microbiol Rev. 1994 Apr.

Abstract

Selected segments of any DNA molecule can be amplified exponentially by PCR. This technique provides a powerful tool to detect and identify minimal numbers of microorganisms. PCR is applicable both in diagnosis and in epidemiology. By amplification of hypervariable DNA domains, differences can be detected even among closely related strains. PCR fingerprinting is a valuable tool for medical microbiologists, epidemiologists, and microbial taxonomists. The current state of PCR-mediated genotyping is reviewed, and a comparison with conventional molecular typing methods is included. Because of its speed and versatility, PCR fingerprinting will play an important role in microbial genetics, epidemiology, and systematics.

PubMed Disclaimer

Similar articles

• DNA- and PCR-fingerprinting in fungi.
  Meyer W, Lieckfeldt E, Kuhls K, Freedman EZ, Börner T, Mitchell TG. Meyer W, et al. EXS. 1993;67:311-20. doi: 10.1007/978-3-0348-8583-6_28. EXS. 1993. PMID: 8400701
• [Molecular methods and techniques used in diagnosis and epidemiology of infections caused by pathogenic fungi].
  Dobrowolska A, Bojarski L, Staczek P. Dobrowolska A, et al. Wiad Parazytol. 2002;48(3):241-55. Wiad Parazytol. 2002. PMID: 16888969 Review. Polish.
• [PCR genetic typing of pathogenic microorganisms].
  Shaginian IA, Gintsburg AL. Shaginian IA, et al. Genetika. 1995 May;31(5):600-10. Genetika. 1995. PMID: 7622023 Review. Russian.
• Generation of DNA probes for detection of microorganisms by polymerase chain reaction fingerprinting.
  Giesendorf BA, Quint WG, Vandamme P, van Belkum A. Giesendorf BA, et al. Zentralbl Bakteriol. 1996 Apr;283(4):417-30. doi: 10.1016/s0934-8840(96)80121-6. Zentralbl Bakteriol. 1996. PMID: 8737942 Review.
• Hybridization probes for conventional DNA fingerprinting used as single primers in the polymerase chain reaction to distinguish strains of Cryptococcus neoformans.
  Meyer W, Mitchell TG, Freedman EZ, Vilgalys R. Meyer W, et al. J Clin Microbiol. 1993 Sep;31(9):2274-80. doi: 10.1128/jcm.31.9.2274-2280.1993. J Clin Microbiol. 1993. PMID: 8408543 Free PMC article.

Cited by

• Molecular epidemiology of fluoroquinolone-resistant Escherichia coli bloodstream isolates from patients admitted to European cancer centers.
  Oethinger M, Conrad S, Kaifel K, Cometta A, Bille J, Klotz G, Glauser MP, Marre R, Kern WV. Oethinger M, et al. Antimicrob Agents Chemother. 1996 Feb;40(2):387-92. doi: 10.1128/AAC.40.2.387. Antimicrob Agents Chemother. 1996. PMID: 8834885 Free PMC article.
• Development of a LAMP assay using a portable device for the real-time detection of cotton leaf curl disease in field conditions.
  Rafiq A, Ali WR, Asif M, Ahmed N, Khan WS, Mansoor S, Bajwa SZ, Amin I. Rafiq A, et al. Biol Methods Protoc. 2021 May 6;6(1):bpab010. doi: 10.1093/biomethods/bpab010. eCollection 2021. Biol Methods Protoc. 2021. PMID: 34084942 Free PMC article.
• Penicillium marneffei: types and drug susceptibility.
  Imwidthaya P, Thipsuvan K, Chaiprasert A, Danchaivijitra S, Sutthent R, Jearanaisilavong J. Imwidthaya P, et al. Mycopathologia. 2001;149(3):109-15. doi: 10.1023/a:1007245226495. Mycopathologia. 2001. PMID: 11307592
• Unlocking the molecular realm: advanced approaches for identifying clinically and environmentally relevant bacteria.
  Silva MRFD, Souza K, Bezerra T, Silva T, Fernades D, Silva F, Araújo L, Almeida A, Oliveira M. Silva MRFD, et al. Braz J Med Biol Res. 2023 Oct 13;56:e12894. doi: 10.1590/1414-431X2023e12894. eCollection 2023. Braz J Med Biol Res. 2023. PMID: 37851790 Free PMC article.
• Multilocus Sequence Typing of Non-JP2 Serotype b Aggregatibacter actinomycetemcomitans Strains of Ghanaian and Swedish Origin.
  Claesson R, Johansson A, Höglund Åberg C, Esberg A, Haubek D, Oscarsson J. Claesson R, et al. Front Cell Infect Microbiol. 2021 Dec 14;11:769671. doi: 10.3389/fcimb.2021.769671. eCollection 2021. Front Cell Infect Microbiol. 2021. PMID: 34970507 Free PMC article.

References

1. 1. Nucleic Acids Res. 1991 Dec 25;19(24):6823-31 - PubMed
2. 1. Science. 1991 Dec 20;254(5039):1735-9 - PubMed
3. 1. Nucleic Acids Res. 1992 Feb 11;20(3):581-6 - PubMed
4. 1. Clin Infect Dis. 1992 Mar;14 Suppl 1:S4-10 - PubMed
5. 1. Curr Genet. 1991 Nov;20(5):391-6 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Medical

  • MedlinePlus Health Information