Molecular characterization of PulE, a protein required for pullulanase secretion - PubMed (original) (raw)
Comparative Study
Molecular characterization of PulE, a protein required for pullulanase secretion
O Possot et al. Mol Microbiol. 1994 Apr.
Abstract
pulE, one of 14 genes specifically required for pullulanase secretion in Klebsiella oxytoca, codes for a putative nucleotide-binding protein. Subcellular fractionation indicated that the majority of PulE in Escherichia coli cells expressing all 14 secretion genes is mainly associated with the cytoplasmic membrane through both hydrophobic and non-hydrophobic interactions. Mutational analysis revealed that one of the two regions of PulE that are conserved in many nucleotide-binding proteins (Walker box A) is essential for pullulanase secretion. Likewise, mutations that removed aspartate residues from each of two regions immediately downstream from the Walker box A also reduced secretion. These aspartate-rich regions are highly conserved in all 16 known PulE homologues but not in any other nucleotide-binding proteins. Altogether, these results indicate that PulE might belong to a new family of nucleotide-binding proteins. The protein could not be cross-linked to the photoactivatable ATP analogue azido-ATP, however. Most pulE point or deletion mutations which prevented pullulanase secretion exhibited transdominance when expressed at high levels in cells producing wild-type PulE protein. Evidence presented suggests that PulE might be a homodimer.
Similar articles
- Pullulanase secretion in Escherichia coli K-12 requires a cytoplasmic protein and a putative polytopic cytoplasmic membrane protein.
Possot O, d'Enfert C, Reyss I, Pugsley AP. Possot O, et al. Mol Microbiol. 1992 Jan;6(1):95-105. doi: 10.1111/j.1365-2958.1992.tb00841.x. Mol Microbiol. 1992. PMID: 1738317 - The conserved tetracysteine motif in the general secretory pathway component PulE is required for efficient pullulanase secretion.
Possot OM, Pugsley AP. Possot OM, et al. Gene. 1997 Jun 11;192(1):45-50. doi: 10.1016/s0378-1119(97)00009-7. Gene. 1997. PMID: 9224873 - Multiple interactions between pullulanase secreton components involved in stabilization and cytoplasmic membrane association of PulE.
Possot OM, Vignon G, Bomchil N, Ebel F, Pugsley AP. Possot OM, et al. J Bacteriol. 2000 Apr;182(8):2142-52. doi: 10.1128/JB.182.8.2142-2152.2000. J Bacteriol. 2000. PMID: 10735856 Free PMC article. - The general secretory pathway of Klebsiella oxytoca: no evidence for relocalization or assembly of pilin-like PulG protein into a multiprotein complex.
Pugsley AP, Possot O. Pugsley AP, et al. Mol Microbiol. 1993 Nov;10(3):665-74. doi: 10.1111/j.1365-2958.1993.tb00938.x. Mol Microbiol. 1993. PMID: 7968543 - Identification and preliminary characterization of temperature-sensitive mutations affecting HlyB, the translocator required for the secretion of haemolysin (HlyA) from Escherichia coli.
Blight MA, Pimenta AL, Lazzaroni JC, Dando C, Kotelevets L, Séror SJ, Holland IB. Blight MA, et al. Mol Gen Genet. 1994 Nov 15;245(4):431-40. doi: 10.1007/BF00302255. Mol Gen Genet. 1994. PMID: 7808392
Cited by
- Multiple conformations facilitate PilT function in the type IV pilus.
McCallum M, Benlekbir S, Nguyen S, Tammam S, Rubinstein JL, Burrows LL, Howell PL. McCallum M, et al. Nat Commun. 2019 Nov 15;10(1):5198. doi: 10.1038/s41467-019-13070-z. Nat Commun. 2019. PMID: 31729381 Free PMC article. - Pullulanase Is Necessary for the Efficient Intracellular Growth of Francisella tularensis.
Uda A, Sharma N, Takimoto K, Deyu T, Koyama Y, Park ES, Fujita O, Hotta A, Morikawa S. Uda A, et al. PLoS One. 2016 Jul 22;11(7):e0159740. doi: 10.1371/journal.pone.0159740. eCollection 2016. PLoS One. 2016. PMID: 27448164 Free PMC article. - Editorial.
Filloux A. Filloux A. FEMS Microbiol Rev. 2015 Jan;39(1):1. doi: 10.1093/femsre/fuu005. FEMS Microbiol Rev. 2015. PMID: 25793962 No abstract available. - Exceptionally widespread nanomachines composed of type IV pilins: the prokaryotic Swiss Army knives.
Berry JL, Pelicic V. Berry JL, et al. FEMS Microbiol Rev. 2015 Jan;39(1):134-54. doi: 10.1093/femsre/fuu001. Epub 2014 Dec 4. FEMS Microbiol Rev. 2015. PMID: 25793961 Free PMC article. Review. - Characterization and localization of the Campylobacter jejuni transformation system proteins CtsE, CtsP, and CtsX.
Beauchamp JM, Erfurt RS, DiRita VJ. Beauchamp JM, et al. J Bacteriol. 2015 Feb;197(3):636-45. doi: 10.1128/JB.02434-14. Epub 2014 Dec 1. J Bacteriol. 2015. PMID: 25448813 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources