A switch from stromal to tumor cell expression of stromelysin-1 mRNA associated with the conversion of squamous to spindle carcinomas during mouse skin tumor progression - PubMed (original) (raw)

A switch from stromal to tumor cell expression of stromelysin-1 mRNA associated with the conversion of squamous to spindle carcinomas during mouse skin tumor progression

J H Wright et al. Mol Carcinog. 1994 Aug.

Abstract

We previously reported that the expression of stromelysin-1 (ST-1), a matrix-degrading metalloproteinase, correlates with tumor progression in the mouse skin model of carcinogenesis. Using in situ hybridization techniques, we confirmed in this study the expression of ST-1 mRNA in mouse skin keratinocytes treated with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate and also observed dramatic expression of ST-1 message in underlying fibroblastic cells. Benign tumors formed by an initiation/promotion protocol expressed low levels of ST-1 mRNA, which was localized exclusively to stromal tissue surrounding the tumor cells. Squamous cell carcinomas, produced either by chemical carcinogenesis or by injection of cultured cells derived from chemically initiated squamous cell tumors, expressed high levels of ST-1 mRNA, which was also localized to adjacent stromal tissues. In contrast, aggressive, highly metastatic spindle cell tumors expressed ST-1 mRNA in the tumor cells as well as in normal, adjacent stroma. These results suggest that the change from ST-1 expression in surrounding stromal cells to its expression in the tumor cells themselves is associated with the conversion of squamous to spindle carcinomas and may play a causal role in the ability of these cells to invade and metastasize.

PubMed Disclaimer

Cited by

Both Full-Length and Protease-Cleaved Products of Osteopontin Are Elevated in Infectious Diseases.
Hattori T, Iwasaki-Hozumi H, Bai G, Chagan-Yasutan H, Shete A, Telan EF, Takahashi A, Ashino Y, Matsuba T. Hattori T, et al. Biomedicines. 2021 Aug 13;9(8):1006. doi: 10.3390/biomedicines9081006. Biomedicines. 2021. PMID: 34440210 Free PMC article. Review.
Stromal cyclin D1 promotes heterotypic immune signaling and breast cancer growth.
Pestell TG, Jiao X, Kumar M, Peck AR, Prisco M, Deng S, Li Z, Ertel A, Casimiro MC, Ju X, Di Rocco A, Di Sante G, Katiyar S, Shupp A, Lisanti MP, Jain P, Wu K, Rui H, Hooper DC, Yu Z, Goldman AR, Speicher DW, Laury-Kleintop L, Pestell RG. Pestell TG, et al. Oncotarget. 2017 Aug 4;8(47):81754-81775. doi: 10.18632/oncotarget.19953. eCollection 2017 Oct 10. Oncotarget. 2017. PMID: 29137220 Free PMC article.
The gene expression profiles of canine mammary cancer cells grown with carcinoma-associated fibroblasts (CAFs) as a co-culture in vitro.
Król M, Pawłowski KM, Szyszko K, Maciejewski H, Dolka I, Manuali E, Jank M, Motyl T. Król M, et al. BMC Vet Res. 2012 Mar 27;8:35. doi: 10.1186/1746-6148-8-35. BMC Vet Res. 2012. PMID: 22453032 Free PMC article.
Keratinocyte expression of MMP3 enhances differentiation and prevents tumor establishment.
McCawley LJ, Wright J, LaFleur BJ, Crawford HC, Matrisian LM. McCawley LJ, et al. Am J Pathol. 2008 Nov;173(5):1528-39. doi: 10.2353/ajpath.2008.080132. Epub 2008 Oct 2. Am J Pathol. 2008. PMID: 18832569 Free PMC article.
Clinical implications of matrix metalloproteinases.
Mandal M, Mandal A, Das S, Chakraborti T, Sajal C. Mandal M, et al. Mol Cell Biochem. 2003 Oct;252(1-2):305-29. doi: 10.1023/a:1025526424637. Mol Cell Biochem. 2003. PMID: 14577606 Review.

A switch from stromal to tumor cell expression of stromelysin-1 mRNA associated with the conversion of squamous to spindle carcinomas during mouse skin tumor progression - PubMed (original) (raw)